Template:SUIT low ADP NADH

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In the presence of endogenous substrates, the addition of a low concentration of ADP (0.1 ΞΌM) is a crucial step to detect the fully oxidized NAD for the calculation of the NAD redox state. ADP stimulates the consumption of endogenous substrates, which can be observed as a decrease in respiration and the NADH fluorescence signal.

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