Difference between revisions of "Template:SUIT text D003"
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The SUIT-001 O2 ce-pce D003 protocol in combination with [[SUIT-002 O2 ce-pce D007]] is specially designed to provide a common reference for comparison of respiratory control of [[Permeabilized cells| permeabilized cells]] (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of cell types. SUIT-001 O2 ce-pce D003 gives information on [[ROUTINE]] respiration (ce: non-permeabilized cells) and after the plasma membrane permeabilization (pce: permeabilized cells) on linear coupling control ([[LEAK-respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with NADH linked-substrates ([[PM pathway control state|PM]]). Moreover, the pathway control in ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[FNS]], [[Succinate pathway control state| S]] and [[SGp-pathway control state| SGp]] pathways) can be evaluated by using this SUIT protocol. SUIT-001 O2 ce-pce D003 can be extended with the CIV assay module. | The SUIT-001 O2 ce-pce D003 protocol in combination with [[SUIT-002 O2 ce-pce D007]] is specially designed to provide a common reference for comparison of respiratory control of [[Permeabilized cells| permeabilized cells]] (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of cell types. SUIT-001 O2 ce-pce D003 gives information on [[ROUTINE]] respiration (ce: non-permeabilized cells) and after the plasma membrane permeabilization (pce: permeabilized cells) on linear coupling control ([[LEAK-respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']]) with NADH linked-substrates ([[PM-pathway control state|PM]]). Moreover, the pathway control in ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[FNS]], [[Succinate pathway control state| S]] and [[SGp-pathway control state| SGp]] pathways) can be evaluated by using this SUIT protocol. SUIT-001 O2 ce-pce D003 can be extended with the CIV assay module. | ||
Revision as of 14:58, 3 June 2020
The SUIT-001 O2 ce-pce D003 protocol in combination with SUIT-002 O2 ce-pce D007 is specially designed to provide a common reference for comparison of respiratory control of permeabilized cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of cell types. SUIT-001 O2 ce-pce D003 gives information on ROUTINE respiration (ce: non-permeabilized cells) and after the plasma membrane permeabilization (pce: permeabilized cells) on linear coupling control (L- P- E) with NADH linked-substrates (PM). Moreover, the pathway control in ET state (N, NS, FNS, S and SGp pathways) can be evaluated by using this SUIT protocol. SUIT-001 O2 ce-pce D003 can be extended with the CIV assay module.
