Thayer 1975 J Biol Chem

Has title::Thayer WS, Hinkle PC (1975) Synthesis of adenosine triphosphate by an artificially imposed electrochemical proton gradient in bovine heart submitochondrial particles. J Biol Chem 250: 5330-5335.

» [[Has info::PMID: 237916 Open Access]]

Was written by::Thayer WS, Was written by::Hinkle PC (Was published in year::1975) Was published in journal::J Biol Chem

Abstract: [[has abstract::Submitochondrial particles subjected to an artificially imposed electrochemical proton gradient consisting of a pH gradient (acid to base transition) and membrane potential (low to high K^-+ transition in the presence of valinomycin) catalyzed the net synthesis of 2.5 nmol of [-32P]ATP per mg of protein from ADP and 32-Pi. Optimal reaction conditions included incubation of submitochondrial particles in malonate at pH 5.0 with valinomycin in the absence of added K^-+, followed by a rapid transition to pH 7.5 and 100 mM K^-+. ATP synthesis continued for about 6 s and was sensitive to uncouplers or oligomycin but insensitive to inhibitors of electron transport. Lower amounts of ATP were formed by either the pH gradient (25%) of K^-+ gradient (15%) alone. These results demonstrate that an electrochemical gradient of protons can drive the synthesis of ATP by reversal of the proton-translocating ATPase independent of electron transport.]] • Keywords: has publicationkeywords::ATP synthesis, has publicationkeywords::Proton gradient, has publicationkeywords::Submitochondrial particles, has publicationkeywords::Beef heart

Labels: MiParea: MiP area::Respiration 

Organism: Organism::Bovines  Tissue;cell: tissue and cell::Heart  Preparation: Preparation::SMP 

Regulation: Topic::ATP; ADP; AMP; PCr, Topic::Coupling efficiency; uncoupling, Topic::Ion homeostasis, Topic::pH  Coupling state: Coupling states::OXPHOS