Uribe-Alvarez 2016 Abstract MitoFit Science Camp 2016: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=In ''Staphylococcus epidermidis'', oxygen variations promote differential expression of respiratory enzymes that constitute possible therapeutic targets. | |||
|info=[[File:Uribe-AlvarezC.jpg|120px|right|Cristina Uribe-Alvarez]] | |||
|authors=Uribe-Alvarez C, Chiquete-Felix N, Contreras-Zentella M, Guerrero-Castillo S, Pena A, Uribe-Carvajal S | |||
|year=2016 | |year=2016 | ||
|event=MitoFit Science Camp 2016 Kuehtai AT | |event=MitoFit Science Camp 2016 Kuehtai AT | ||
|abstract=''Staphylcoccus epidermidis'' does not invade healthy tissues, however, it has been identified as a cause of nosocomial infections due to its ability to form biofilms on polymer surfaces [1]. ''S. epidermidis'' can be grown at different oxygen concentrations ([O<sub>2</sub>]), including mammalian skin where [O<sub>2</sub>] ranges from 3-5% and in anaerobic altered tissues [2,3]. | |||
Biofilm formation of ''S. epidermidis'' and its respiratory chain components grown in aerobic, microaerobic and anaerobic conditions were evaluated by in-gel activities, enzymatic activities, spectrophotometry and oxymetry. | |||
Varying [O<sub>2</sub>] modified both biofilm formation and the components in the respiratory chain: At high [O<sub>2</sub>], little tendency to form biofilms was observed. ''S. epidermidis'' expressed glycerol-3-phosphate, pyruvate, ethanol and succinate dehydrogenases; and cyt bo and aa3. Under micro-aerobiosis, biofilm formation increased slightly; pyruvate, ethanol, glycerol-3-phosphate and succinate dehydrogenase decreased; aa3 cyt was not detected; Under anaerobiosis high biofilm-formation and low ethanol and pyruvate dehydrogenase activities were found; anaerobic nitrate dehydrogenase activity was detected. Aerobic-grown cells with cyanide increased biofilm formation. Anaerobic-grown cells with methylamine decreased biofilm formation. | |||
Thus, either a decrease in [O<sub>2</sub>] or the inhibition of the aerobic chain led ''S. epidermidis'' to associate into biofilms. In contrast, high [O<sub>2</sub>] or inhibition of the anaerobic nitrate reductase prevented biofilm formation suggesting that the enzymes expressed at low to null [O<sub>2</sub>] are therapeutic targets against biofilm formation by ''S. epidermidis''. | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|taxonomic group=Eubacteria | |||
|topics=Oxygen kinetics | |||
|additional=MitoFit Science Camp 2016 | |additional=MitoFit Science Camp 2016 | ||
}} | }} | ||
== Affiliations == | == Affiliations == | ||
1- . - | 1-Dept Mol Genet, IFC, UNAM, Mexico; 2-Dept Cell Developm Biol, IFC, UNAM, Mexico, 3-Nijmegen Center Mitochondrial Disorders, Radboud Univ Med Center, The Netherlands. - [email protected] | ||
== References == | == References == | ||
# | #Otto M (2009) ''Staphylococcus epidermidis''-the 'accidental' pathogen. Nat Rev Microbiol 7:555-67. | ||
#Peyssonnaux C, Boutin AT, Zinkernagel AS, Datta V, Nizet V, Johnson RS (2008) Critical role of HIF-1alpha in keratinocyte defense against bacterial infection. J Invest Dermatol 128:1964-8. | |||
#Wiese M, Gerlach RG, Popp I, Matuszak J, Mahapatro M, Castiglione K, Chakravortty D, Willam C, Hensel M, Bogdan C, Jantsch J (2012) Hypoxia-mediated impairment of the mitochondrial respiratory chain inhibits the bactericidal activity of macrophages. Infect Immun 80:1455-66. | |||
# | # |
Revision as of 08:35, 6 June 2016
In Staphylococcus epidermidis, oxygen variations promote differential expression of respiratory enzymes that constitute possible therapeutic targets. |
Link:
Uribe-Alvarez C, Chiquete-Felix N, Contreras-Zentella M, Guerrero-Castillo S, Pena A, Uribe-Carvajal S (2016)
Event: MitoFit Science Camp 2016 Kuehtai AT
Staphylcoccus epidermidis does not invade healthy tissues, however, it has been identified as a cause of nosocomial infections due to its ability to form biofilms on polymer surfaces [1]. S. epidermidis can be grown at different oxygen concentrations ([O2]), including mammalian skin where [O2] ranges from 3-5% and in anaerobic altered tissues [2,3].
Biofilm formation of S. epidermidis and its respiratory chain components grown in aerobic, microaerobic and anaerobic conditions were evaluated by in-gel activities, enzymatic activities, spectrophotometry and oxymetry. Varying [O2] modified both biofilm formation and the components in the respiratory chain: At high [O2], little tendency to form biofilms was observed. S. epidermidis expressed glycerol-3-phosphate, pyruvate, ethanol and succinate dehydrogenases; and cyt bo and aa3. Under micro-aerobiosis, biofilm formation increased slightly; pyruvate, ethanol, glycerol-3-phosphate and succinate dehydrogenase decreased; aa3 cyt was not detected; Under anaerobiosis high biofilm-formation and low ethanol and pyruvate dehydrogenase activities were found; anaerobic nitrate dehydrogenase activity was detected. Aerobic-grown cells with cyanide increased biofilm formation. Anaerobic-grown cells with methylamine decreased biofilm formation.
Thus, either a decrease in [O2] or the inhibition of the aerobic chain led S. epidermidis to associate into biofilms. In contrast, high [O2] or inhibition of the anaerobic nitrate reductase prevented biofilm formation suggesting that the enzymes expressed at low to null [O2] are therapeutic targets against biofilm formation by S. epidermidis.
Labels:
Regulation: Oxygen kinetics
MitoFit Science Camp 2016
Affiliations
1-Dept Mol Genet, IFC, UNAM, Mexico; 2-Dept Cell Developm Biol, IFC, UNAM, Mexico, 3-Nijmegen Center Mitochondrial Disorders, Radboud Univ Med Center, The Netherlands. - [email protected]
References
- Otto M (2009) Staphylococcus epidermidis-the 'accidental' pathogen. Nat Rev Microbiol 7:555-67.
- Peyssonnaux C, Boutin AT, Zinkernagel AS, Datta V, Nizet V, Johnson RS (2008) Critical role of HIF-1alpha in keratinocyte defense against bacterial infection. J Invest Dermatol 128:1964-8.
- Wiese M, Gerlach RG, Popp I, Matuszak J, Mahapatro M, Castiglione K, Chakravortty D, Willam C, Hensel M, Bogdan C, Jantsch J (2012) Hypoxia-mediated impairment of the mitochondrial respiratory chain inhibits the bactericidal activity of macrophages. Infect Immun 80:1455-66.