User talk:Spitzer Gudrun: Difference between revisions
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= Asc gutest 1 | == Asc gutest 1== | ||
:::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1. | :::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1. | ||
| Line 27: | Line 25: | ||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe | ||
::::* Final concentration: 2 mM. | ::::* Final concentration: 2 mM. | ||
== | == Asc gutest 2 == | ||
:::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1. | :::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1. | ||
| Line 59: | Line 56: | ||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe. | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe. | ||
::::* Final concentration: 2 mM. | |||
== Version 3 == | |||
:::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1. | |||
:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span> | |||
=== 2-mL O2k-chamber === | |||
::: '''Preparation of 800 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-chamber''': | |||
::::# Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O | |||
::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2). | |||
::::# Divide into 0.2 mL portions. | |||
::::# Store frozen at -20 °C. | |||
:::»'''O2k manual titrations:''' [[MiPNet09.12]] | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe. | |||
::::* Final concentration: 2 mM. | |||
=== 0.5-mL O2k-chamber === | |||
::: '''Preparation of 1000 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''0.5-mL O2k-chamber''': | |||
::::# Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O | |||
::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2). | |||
::::# Divide into 0.2 mL portions. | |||
::::# Store frozen at -20 °C. | |||
:::»'''O2k manual titrations:''' [[MiPNet09.12]] | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe. | |||
::::* Final concentration: 2 mM. | ::::* Final concentration: 2 mM. | ||
Revision as of 10:09, 22 October 2020
Asc gutest 1
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
'
- Preparation of 800 mM stock solution (dissolved in H2O) for use in 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- Preparation of 1000 mM stock solution (dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 µL of 800mM stock solution using a 25 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe
- Final concentration: 2 mM.
Asc gutest 2
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
- Preparation of 800 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 µL of 800 mM stock solution using a 25 µL Hamilton syringe.
- Final concentration: 2 mM.
- Preparation of 1000 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe.
- Final concentration: 2 mM.
Version 3
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
2-mL O2k-chamber
- Preparation of 800 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 2 mM.
0.5-mL O2k-chamber
- Preparation of 1000 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 2 mM.
