Difference between revisions of "User talk:Tindle Lisa/My sandbox O2k-Virtual support"
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=== O2k-Advanced:Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes | === O2k-Advanced: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes === | ||
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Revision as of 17:49, 1 August 2023
User talk:Tindle Lisa/My sandbox O2k-Virtual support |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
O2k-Vitual support include 8 individual training hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (OroboPOS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyse and discuss your first experimental DatLab files obtained with your O2k with the bioenergetics experts of Oroboros. Instrumental and biological experiments demonstrate the unique advantages and limitations of monitoring of oxygen concentration and respiration, simultaneously with monitoring hydrogen peroxide production and several other MultiSensor options. It offers flexibility to participants and gives the option to choose virtual coaching sessions that best fit individual needs.
General information
- Each O2k-Package includes a remote installation and startup support session with an Oroboros expert. This online support session will focus on:
- Instrumental setup
- Quality control
- Discussion with our expert
- Each O2k-Package includes a remote installation and startup support session with an Oroboros expert. This online support session will focus on:
- We encourage new customers to book their virtual session for the instrumental setup before setting up their O2k.
- The virtual coaching sessions for the Installation and startup support (included with O2k-Package purchase) can be used during any time within the O2k-Warranty period (max.3 years). The virtual coaching sessions will take place on the dates to be confirmed both by the participants and tutors. Outside of the warranty period or if more hours are needed, a separate O2k-Virutual support (8 hours of virtual coaching sessions) can be purchased.
O2k-Virtual support session/Installation and startup support session workflow
- During the Installation and startup support session, self-study material is available, including the O2k-Manual and O2k-Videosupport (which are always available on the website).
- It is highly recommended that participants prepare for their coaching session by going through the self-study material.
- » The date of the coaching session will be arranged between the participants and tutor once a registration form is received.
- » The links to the online material are available here: Installation and startup support session self-study material
Virtual coaching hour allocation
- Individual coaching hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants to plan their available 8 hours efficiently. Sessions are marked with asterisks to indicate the level of recommendation for beginners.
- The duration is estimated for participants who still do not have experience with the O2k, sessions might be faster for experienced O2k users.
- More than one topic can be covered in a single session and the minimum time per session is 1 hour.
- The virtual coaching sessions will take place on the dates confirmed by the participants and tutor.
Materials for self-study
- The materials for self-study, with the names and links to materials, are recommended to be studied before the respective O2k-Virtual support coaching sessions.
- » Virtual O2k-Workshop self-study material
Program
- Participants can use the below sample program as a guideline for possible sessions for their online coaching.
- For beginners we recommend to start the virtual coaching with the Installation and startup support session session (Part 1 in the program). Advanced users may choose to select sessions from both the O2k-Basic and Advanced programs.
O2k-Basic
Session | Duration |
---|---|
Part 1: Installation and startup support session | |
Part 1.1: OroboPOS service and O2k instrumental setup | |
Hands-on: OroboPOS service
|
Start-up 2 h * |
Hands-on: O2k instrumental setup
|
Start-up 2 h * |
Part 1.2: DatLab | |
DatLab overview | Start-up 1 h * |
Part 1.3: O2 calibration and instrumental background | |
Hands-on: Quality control 1: Oxygen calibration
|
Do-it-yourself 1.5 h |
Hands-on: Quality control 2: Oxygen background
|
Do-it-yourself 2 h |
DatLab analysis and discussion | Start-up 1 h * |
Part 2 | |
Part 2.1: Biological samples and experimental design | |
Discussion about biological samples, experimental design, SUIT protocols
|
Start-up 1-2 h * |
Part 2.2: Biological experiment and data analysis | |
Hands-on: Quality control 1: Oxygen calibration
|
Do-it-yourself 1.5 h |
Hands-on: Biological experiment: cell or mitochondrial respiration
|
Do-it-yourself 1-3 h |
Hands-on: O2k-cleaning after use
|
Do-it-yourself 1 h |
Hands-on: DatLab analysis and DatLab performance evaluation. Discussion |
Start-up 2 h* |
O2k-Advanced: Simultaneous determination of O2 and H2O2 fluxes
Session | Duration
|
---|---|
Introduction to H2O2 measurements and discussion
|
1 h * |
Hands-on: Quality control 1: Oxygen calibration
|
1.5 h *
|
Hands-on: Amplex UltraRed calibration: Amplex UltraRed calibration in the absence of biological sample.
|
1 h |
Hands-on: Biological experiment: simultaneous measurement of O2 and H2O2 production
|
2 h |
Hands-on: O2k-cleaning after use
|
1 h * |
Hands-on: DatLab H2O2 analysis and DatLab performance evaluation. Discussion |
1.5 h* |
Contact
- Oroboros Instruments
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and Cell Research