Difference between revisions of "User talk:Tindle Lisa/My sandbox O2k-Virtual support"

Latest revision as of 12:21, 7 November 2023

User talk:Tindle Lisa/My sandbox O2k-Virtual support

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

O2k-Virtual support includes 8 individual hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyze and discuss your experimental DatLab files obtained with your O2k with the bioenergetics experts of Oroboros. It offers flexibility to participants and gives the option to choose virtual sessions that best fit individual needs.

O2k-Virtual support hour allocation

Individual hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants plan their available 8 hours efficiently. Sessions marked with asterisks indicate ones recommended for beginners.

The duration is estimated for participants who still do not have experience with the O2k, sessions might take less time for experienced O2k users.

More than one topic can be covered in a single session and the minimum time per session is 1 hour.

The O2k-Virtual support sessions will take place on the dates confirmed by the participants and tutor.

Materials for self-study

It is highly recommended that participants prepare for their sessions by going through the self-study material.

» Virtual O2k-Workshop self-study material

Program

Participants can use the below sample program as a guideline for possible sessions.

For beginners we recommend starting with the Installation and startup support session session (Part 1 in the program). Experienced users may choose to select sessions from both the O2k-Basic and Advanced programs.

O2k-Basic

Session	Duration
Part 1: Installation and startup support session
Part 1.1: POS service and O2k instrumental setup
Hands-on: Installation of the O2k 1. O2k-chamber assembly 2. POS service: a. Cathode cleaning b. Anode cleaning c. Membrane mounting 3. Insert POS 4. Volume calibration	2.5 h
Part 1.2: DatLab 8 overview and O₂ calibration
DatLab overview Hands-on: Quality control 1: Oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	1.5 h
Part 1.3: O₂ background
Hands-on: Quality control 2: Oxygen background Select one DL-Protocol according to your needs: Instrumental O2 background TiP2k Instrumental O2 background manual injections Instrumental high O2 background TiP2k Instrumental high O2 background manual injections	Do-it-yourself 2 h
DatLab analysis and discussion	Start-up 1 h

Part 2
Part 2.1: Biological samples and experimental design
Discussion about biological samples, experimental design, SUIT protocols Prepare by reading “Materials for self-study” Section 2.1	1-2 h
Part 2.2: Biological experiment and data analysis
Hands-on: Quality control 1: Oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Do-it-yourself 1.5 h
Hands-on: Biological experiment: cell or mitochondrial respiration DL-Protocol: SUIT protocol will be selected/discussed individually	Do-it-yourself 1-3 h
Hands-on: O2k-cleaning after use Select one DL-Protocol according to your needs: O2k-cleaning AfterUse O2k-cleaning AfterUse inhibitors O2k-cleaning AfterUse stirrers	Do-it-yourself 1 h
Hands-on: DatLab analysis and DatLab performance evaluation. Discussion	1-2 h

O2k-Advanced: Simultaneous determination of O₂ and H₂O₂ fluxes

Some of the hours from the basic program can be shortened or replaced by advanced sessions:

Session	Duration
Introduction to H₂O₂ measurements and discussion Get prepared with “Materials for self-study”, “O2k-Advanced, O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes”	1 h
Hands-on: Quality control 1: Oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Do-it-yourself 1.5 h
Hands-on: Amplex UltraRed calibration: Amplex UltraRed calibration in the absence of biological sample. DL-Protocol: AmR calibration	1 h
Hands-on: Biological experiment: simultaneous measurement of O₂ and H₂O₂ production Select one DL-Protocol according to your needs: Suggested SUIT protocol: SUIT-026 DL-Protocol: SUIT-026 AmR mt D064	Do-it-yourself2 h
Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse	Do-it-yourself1 h
Hands-on: DatLab H₂O₂ analysis and DatLab performance evaluation. Discussion	1.5 h

Contact

[email protected]

Oroboros Instruments

High-Resolution Respirometry

Schoepfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and Cell Research

@@ Line 5: / Line 5: @@
 {{MitoPedia without banner
 |abbr=
-|description=O2k-Vitual support include 8 individual training hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (OroboPOS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyse and discuss your first experimental [[DatLab]]  files obtained with your O2k with the bioenergetics experts of Oroboros. Instrumental and biological experiments demonstrate the unique advantages and limitations of monitoring of oxygen concentration and respiration, simultaneously with monitoring hydrogen peroxide production and several other MultiSensor options. It offers flexibility to participants and gives the option to choose virtual coaching sessions that best fit individual needs.
+|description=O2k-Virtual support includes 8 individual hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyze and discuss your experimental [[DatLab]]  files obtained with your O2k with the bioenergetics experts of Oroboros. It offers flexibility to participants and gives the option to choose virtual sessions that best fit individual needs.
 |info=
 }}
-== O2k-Virtual support session workflow ==
-:::* During the O2k-Virtual support self-study material is available, including the [[O2k-Manual]] and [[O2k-Videosupport]] (which are always available on the website).
-:::: It is highly recommended that participants prepare for their coaching session by going through the self-study material (e.g. video clips and manuals) of Part 1.1 (OroboPOS service, O2k instrumental setup).
-:::::» The links to the online material are in the "Material for self-study" section below.
-:::::» The date of the coaching session will be arranged between the participants and tutor once a registration form is received.
-== Virtual coaching hour allocation ==
+== O2k-Virtual support hour allocation ==
-:::: Individual coaching hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants to plan their available 10 hours efficiently. Sessions are marked with asterisks to indicate the level of recommendation for beginners.
+:::: Individual hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants plan their available 8 hours efficiently. Sessions marked with asterisks indicate ones recommended for beginners.
-:::: The duration is estimated for participants who still do not have experience with the O2k, sessions might be faster for experienced O2k users.
+:::: The duration is estimated for participants who still do not have experience with the O2k, sessions might take less time for experienced O2k users.
 :::: More than one topic can be covered in a single session and the minimum time per session is 1 hour.
-:::: The virtual coaching sessions will take place on the dates confirmed by the participants and tutor.
+:::: The O2k-Virtual support sessions will take place on the dates confirmed by the participants and tutor.
 == Materials for self-study ==
-:::: The materials for self-study, with the names and links to materials, are recommended to be studied before the respective O2k-Virtual support coaching sessions.
+:::: It is highly recommended that participants prepare for their sessions by going through the self-study material.
 ::::» '''[[Virtual O2k-Workshop self-study material]] '''
 == Program ==
-:::: Participants can use the below sample program as a guideline for possible sessions for their online coaching.
+:::: Participants can use the below sample program as a guideline for possible sessions.
-:::: For beginners we recommend to start the virtual coaching with the start-up session (session 1.1 and 1.2 in the program). Advanced users may choose to select sessions from both the O2k-Basic and Advanced programs.
+:::: For beginners we recommend starting with the Installation and startup support session session (Part 1 in the program). Experienced users may choose to select sessions from both the O2k-Basic and Advanced programs.
+=== O2k-Basic ===
 {| class="wikitable"
 ! Session
 ! Duration
 |-
-| '''Part 1.1: OroboPOS service and O2k instrumental setup'''
+| <big>'''Part 1: Installation and startup support session'''</big>
 |
 |-
-| Hands-on: OroboPOS service <br>
+| '''Part 1.1: POS service and O2k instrumental setup'''
-::# OroboPOS
+|
-::# Cathode cleaning
-::# Anode cleaning
-::# Membrane mounting
-| Start-up 2 h *
 |-
-| Hands-on: O2k instrumental setup  <br>
+| Hands-on: Installation of the O2k <br>
-::# NextGen-O2k all-in-one
+::1. O2k-chamber assembly
-::# Insert SmartPOS
+::2. POS service:
-::# Insert O2k Chamber
+:::::a. Cathode cleaning
-::# Chamber volume calibration
+:::::b. Anode cleaning
-| Start-up 2 h *
+:::::c. Membrane mounting
+::3. Insert POS
+::4. Volume calibration
+| 2.5 h
 |-
-|
+| '''Part 1.2: DatLab 8 overview and O<sub>2</sub> calibration'''
 |
 |-
-| '''Part 1.2: DatLab'''
+| DatLab overview<br>
+Hands-on: Quality control 1: Oxygen calibration <br>
+::# DL-Protocol: O2k-cleaning BeforeUse
+::# DL-Protocol: O2 calibration air
+| 1.5 h
+|-
+| '''Part 1.3: O<sub>2</sub> background'''
 |
 |-
+| Hands-on: Quality control 2: Oxygen background <br>
-| DatLab overview || Start-up 1 h *
+::Select one DL-Protocol according to your needs:
+::# Instrumental O2 background TiP2k
+::# Instrumental O2 background manual injections
+::# Instrumental high O2 background TiP2k
+::# Instrumental high O2 background manual injections
+| '''Do-it-yourself''' 2 h
+|-
+| DatLab analysis and discussion
+| Start-up 1 h
 |-
 |
 |
 |-
-| '''Part 1.3: O<sub>2</sub> calibration and instrumental background'''
+| <big>'''Part 2'''</big>
+|
+|-
+| '''Part 2.1: Biological samples and experimental design'''
+|
+|-
+| Discussion about biological samples, experimental design, SUIT protocols <br>
+:: Prepare by reading “Materials for self-study” Section 2.1
+|  1-2 h
+|-
+| '''Part 2.2: Biological experiment and data analysis '''
 |
 |-
 | Hands-on: Quality control 1: Oxygen calibration <br>
+::# DL-Protocol: O2k-cleaning BeforeUse
+::# DL-Protocol: O2 calibration air
+| '''Do-it-yourself''' 1.5 h
+|-
+| Hands-on: Biological experiment: cell or mitochondrial respiration <br>
+:: DL-Protocol: SUIT protocol will be selected/discussed individually
+| '''Do-it-yourself''' 1-3 h
+|-
+| Hands-on: O2k-cleaning after use <br>
+:: Select one DL-Protocol according to your needs:
+::: O2k-cleaning AfterUse
+::: O2k-cleaning AfterUse inhibitors
+::: O2k-cleaning AfterUse stirrers
+| '''Do-it-yourself''' 1 h
+|-
+| Hands-on: DatLab analysis and DatLab performance evaluation. Discussion<br>
+| 1-2 h
+|-
+|}
+=== O2k-Advanced: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes ===
+:::: Some of the hours from the basic program can be shortened or replaced by advanced sessions:
+{| class="wikitable"
+! Session
+! Duration
+|-
+| '''Introduction to H<sub>2</sub>O<sub>2</sub> measurements and discussion''' <br>
+::Get prepared with “Materials for self-study”, “O2k-Advanced, O2k-Applications: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes”
+| 1 h
+|-
+| '''Hands-on: Quality control 1: Oxygen calibration'''  <br>
 ::# DL-Protocol: O2k-cleaning BeforeUse
 ::# DL-Protocol: O2 calibration air
-| Do-it-yourself 1.5 h
+| '''Do-it-yourself''' 1.5 h
+|-
+| '''Hands-on: Amplex UltraRed calibration:''' Amplex UltraRed calibration in the absence of biological sample. <br>
+:: DL-Protocol: AmR calibration
+| 1 h
 |-
-| Hands-on: Quality control 2: Oxygen background <br>
+| '''Hands-on: Biological experiment: simultaneous measurement of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> production''' <br>
 ::Select one DL-Protocol according to your needs:
-::#Instrumental O2 background TiP2k
+:: Suggested SUIT protocol: [[SUIT-026]]
-::#Instrumental O2 background manual injections
+:: DL-Protocol: SUIT-026 AmR mt D064
-::#Instrumental high O2 background TiP2k
+|  '''Do-it-yourself'''2 h
-::#Instrumental high O2 background manual injections
-| Do-it-yourself 2 h
 |-
-| DatLab analysis and discussion
+| Hands-on: O2k-cleaning after use <br>
-| 1 h *
+:: DL-Protocol: O2k-cleaning AfterUse
+|  '''Do-it-yourself'''1 h
+|-
+| Hands-on: DatLab H<sub>2</sub>O<sub>2</sub> analysis and DatLab performance evaluation. Discussion<br>
+| 1.5 h
 |-
 |}
-:::: [[File:O2k-Virtual support program.jpg|1000px]]
 == Contact ==