Valle 1986 Biochem Biophys Res Commun: Difference between revisions
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{{Publication | {{Publication | ||
|title=Valle VG, Pereira-da-Silva L, Vercesi AE (1986) Undesirable feature of safranine as a probe for mitochondrial membrane potential. Biochem Biophys Res Commun 135: 189-195. | |title=Valle VG, Pereira-da-Silva L, Vercesi AE (1986) Undesirable feature of safranine as a probe for mitochondrial membrane potential. Biochem Biophys Res Commun 135: 189-195. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/3954769 PMID: 3954769] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/3954769 PMID: 3954769] | ||
|authors=Valle VG, Pereira-da-Silva L, Vercesi AE | |authors=Valle VG, Pereira-da-Silva L, Vercesi AE | ||
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|tissues=Liver | |tissues=Liver | ||
|couplingstates=LEAK | |couplingstates=LEAK | ||
|topics=Membrane | |topics=Ion homeostasis, mt-Membrane potential | ||
|additional=Safranine, Ca2+, mt-swelling | |additional=Safranine, Ca2+, mt-swelling | ||
}} | }} | ||
Revision as of 16:12, 6 August 2013
| Valle VG, Pereira-da-Silva L, Vercesi AE (1986) Undesirable feature of safranine as a probe for mitochondrial membrane potential. Biochem Biophys Res Commun 135: 189-195. |
Valle VG, Pereira-da-Silva L, Vercesi AE (1986) Biochem Biophys Res Commun
Abstract: This communication describes experiments showing that safranine, at the concentrations usually employed as a probe of mitochondrial membrane potential, causes significant undesirable side effects on Ca2+ transport by liver mitochondria. The major observations are: (i) safranine potentiates the spontaneous Ca2+ release from liver mitochondria induced by phosphate or acetoacetate. This is paralelled by potentiation of the release of state-4 respiration and of the rate of mitochondrial swelling, indicating a generalized effect of the dye on the mitochondrial membrane; (ii) the efflux of mitochondrial Ca2+ stimulated by hydroperoxide is irreversible in the presence of safranine even if membrane stabilizers such as Mg2+ and ATP are present. It is concluded that the use of safranine to monitor the changes in membrane potential during Ca2+ transport by mitochondria should be avoided or special care be taken.
Labels:
Tissue;cell: Liver
Regulation: Ion homeostasis"Ion homeostasis" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., mt-Membrane potential
Coupling state: LEAK
Safranine, Ca2+, mt-swelling
