Difference between revisions of "Wiberg 2019 Eur Heart J Acute Cardiovasc Care"
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{{Publication | {{Publication | ||
|title=Wiberg S, Stride N, Bro-Jeppesen J, Holmberg MJ, Kjærgaard J, Larsen S, Donnino MW, Hassager C, Dela F (2019) Mitochondrial dysfunction in adults after out-of-hospital cardiac arrest. Eur Heart J Acute Cardiovasc Care | |title=Wiberg S, Stride N, Bro-Jeppesen J, Holmberg MJ, Kjærgaard J, Larsen S, Donnino MW, Hassager C, Dela F (2019) Mitochondrial dysfunction in adults after out-of-hospital cardiac arrest. Eur Heart J Acute Cardiovasc Care 9:S138-44. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/30854867 PMID: 30854867] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/30854867 PMID: 30854867] | ||
|authors=Wiberg S, Stride N, Bro-Jeppesen J, Holmberg MJ, Kjaergaard J, Larsen S, Donnino MW, Hassager C, Dela F | |authors=Wiberg S, Stride N, Bro-Jeppesen J, Holmberg MJ, Kjaergaard J, Larsen S, Donnino MW, Hassager C, Dela F | ||
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Mitochondrial oxidative phosphorylation capacity in skeletal muscle biopsies was reduced in out-of-hospital cardiac arrest patients undergoing targeted temperature management compared to age-matched, healthy controls. The role of mitochondria as risk markers and potential targets for post-resuscitation care remains unknown. | Mitochondrial oxidative phosphorylation capacity in skeletal muscle biopsies was reduced in out-of-hospital cardiac arrest patients undergoing targeted temperature management compared to age-matched, healthy controls. The role of mitochondria as risk markers and potential targets for post-resuscitation care remains unknown. | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=DK Copenhagen Larsen S, DK Copenhagen Dela F | |mipnetlab=DK Copenhagen Larsen S, DK Copenhagen Dela F | ||
}} | }} | ||
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|organism=Human | |organism=Human | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|couplingstates=OXPHOS, ET | |preparations=Permeabilized tissue | ||
|topics=ADP | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, NS | |pathways=N, NS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2019-03 | ||
}} | }} |
Latest revision as of 16:03, 6 December 2021
Wiberg S, Stride N, Bro-Jeppesen J, Holmberg MJ, Kjærgaard J, Larsen S, Donnino MW, Hassager C, Dela F (2019) Mitochondrial dysfunction in adults after out-of-hospital cardiac arrest. Eur Heart J Acute Cardiovasc Care 9:S138-44. |
Wiberg S, Stride N, Bro-Jeppesen J, Holmberg MJ, Kjaergaard J, Larsen S, Donnino MW, Hassager C, Dela F (2019) Eur Heart J Acute Cardiovasc Care
Abstract: While preclinical studies suggest that mitochondria play a pivotal role in ischaemia-reperfusion injury, the knowledge of mitochondrial function in human out-of-hospital cardiac arrest remains scarce. The present study sought to compare oxidative phosphorylation capacity in skeletal muscle biopsies from out-of-hospital cardiac arrest patients to healthy controls.
This was a substudy of a randomised trial comparing targeted temperature management at 33°C versus 36°C for out-of-hospital cardiac arrest patients. Skeletal muscle biopsies were obtained from adult resuscitated comatose out-of-hospital cardiac arrest patients 28 hours after initiation of targeted temperature management, i.e. at target temperature prior to rewarming, and from age-matched healthy controls. Mitochondrial function was analysed by high-resolution respirometry. Maximal sustained respiration through complex I, maximal coupled respiration through complex I and complex II and maximal electron transport system capacity was compared.
A total of 20 out-of-hospital cardiac arrest patients and 21 controls were included in the analysis. We found no difference in mitochondrial function between temperature allocations. We found no difference in complex I sustained respiration between out-of-hospital cardiac arrest and controls (23 (18-26) vs. 22 (19-26) pmol O2/mg/s, P=0.76), whereas coupled complex I and complex II respiration was significantly lower in out-of-hospital cardiac arrest patients versus controls (53 (42-59) vs. 64 (54-68) pmol O2/mg/s, P=0.01). Furthermore, electron transport system capacity was lower in out-of-hospital cardiac arrest versus controls (63 (51-69) vs. 73 (66-78) pmol O2/mg/s, P=0.005).
Mitochondrial oxidative phosphorylation capacity in skeletal muscle biopsies was reduced in out-of-hospital cardiac arrest patients undergoing targeted temperature management compared to age-matched, healthy controls. The role of mitochondria as risk markers and potential targets for post-resuscitation care remains unknown.
• Bioblast editor: Plangger M • O2k-Network Lab: DK Copenhagen Larsen S, DK Copenhagen Dela F
Labels: MiParea: Respiration, Patients
Pathology: Cardiovascular
Stress:Ischemia-reperfusion
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: ADP Coupling state: LEAK, OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k
2019-03