Difference between revisions of "Willis 2021 Sci Rep"
| (2 intermediate revisions by 2 users not shown) | |||
| Line 2: | Line 2: | ||
|title=Willis JR, Hickey AJR, Devaux JBL (2021) Thermally tolerant intertidal triplefin fish (''Tripterygiidae'') sustain ATP dynamics better than subtidal species under acute heat stress. Sci Rep 11:11074. | |title=Willis JR, Hickey AJR, Devaux JBL (2021) Thermally tolerant intertidal triplefin fish (''Tripterygiidae'') sustain ATP dynamics better than subtidal species under acute heat stress. Sci Rep 11:11074. | ||
|info=[https://pubmed.ncbi.nlm.nih.gov/34040122/ PMID: 34040122 Open Access] | |info=[https://pubmed.ncbi.nlm.nih.gov/34040122/ PMID: 34040122 Open Access] | ||
|authors=Jaime R | |authors=Willis Jaime R, Hickey Anthony J R, Devaux Jules B L | ||
|year=2021 | |year=2021 | ||
|journal=Sci Rep | |journal=Sci Rep | ||
|abstract=Temperature is a key factor that affects all levels of organization. Minute shifts away from thermal optima result in detrimental effects that impact growth, reproduction and survival. Metabolic rates of ectotherms are especially sensitive to temperature and for organisms exposed to high acute temperature changes, in particular intertidal species, energetic processes are often negatively impacted. Previous investigations exploring acute heat stress have implicated cardiac mitochondrial function in determining thermal tolerance. The brain, however, is by weight, one of the most metabolically active and arguably the most temperature sensitive organ. It is essentially aerobic and entirely reliant on oxidative phosphorylation to meet energetic demands, and as temperatures rise, mitochondria become less efficient at synthesising the amount of ATP required to meet the increasing demands. This leads to an energetic crisis. Here we used brain homogenate of three closely related triplefin fish species (''Bellapiscis medius'', ''Forsterygion lapillum'', and ''Forsterygion varium'') and measured respiration and ATP dynamics at three temperatures (15, 25 and 30 °C). We found that the intertidal ''B. medius'' and ''F. lapillum'' were able to maintain rates of ATP production above rates of ATP hydrolysis at high temperatures, compared to the subtidal ''F. varium'', which showed no difference in rates at 30 °C. These results showed that brain mitochondria became less efficient at temperatures below their respective species thermal limits, and that energetic surplus of ATP synthesis over hydrolysis narrows. In subtidal species synthesis matches hydrolysis, leaving no scope to elevate ATP supply. | |abstract=Temperature is a key factor that affects all levels of organization. Minute shifts away from thermal optima result in detrimental effects that impact growth, reproduction and survival. Metabolic rates of ectotherms are especially sensitive to temperature and for organisms exposed to high acute temperature changes, in particular intertidal species, energetic processes are often negatively impacted. Previous investigations exploring acute heat stress have implicated cardiac mitochondrial function in determining thermal tolerance. The brain, however, is by weight, one of the most metabolically active and arguably the most temperature sensitive organ. It is essentially aerobic and entirely reliant on oxidative phosphorylation to meet energetic demands, and as temperatures rise, mitochondria become less efficient at synthesising the amount of ATP required to meet the increasing demands. This leads to an energetic crisis. Here we used brain homogenate of three closely related triplefin fish species (''Bellapiscis medius'', ''Forsterygion lapillum'', and ''Forsterygion varium'') and measured respiration and ATP dynamics at three temperatures (15, 25 and 30 °C). We found that the intertidal ''B. medius'' and ''F. lapillum'' were able to maintain rates of ATP production above rates of ATP hydrolysis at high temperatures, compared to the subtidal ''F. varium'', which showed no difference in rates at 30 °C. These results showed that brain mitochondria became less efficient at temperatures below their respective species thermal limits, and that energetic surplus of ATP synthesis over hydrolysis narrows. In subtidal species synthesis matches hydrolysis, leaving no scope to elevate ATP supply. | ||
|keywords=Physiology, Metabolism, Ecology, Ecophysiology | |keywords=Physiology, Metabolism, Ecology, Ecophysiology | ||
|editor=[[Reiswig R]] | |editor=[[Reiswig R]], [[Plangger M]] | ||
|mipnetlab=NZ Auckland Hickey AJ | |mipnetlab=NZ Auckland Hickey AJ | ||
}} | }} | ||
| Line 19: | Line 19: | ||
|pathways=N, NS, ROX | |pathways=N, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2021-07 | |additional=2021-07, MgG | ||
}} | }} | ||
Latest revision as of 16:36, 31 January 2024
| Willis JR, Hickey AJR, Devaux JBL (2021) Thermally tolerant intertidal triplefin fish (Tripterygiidae) sustain ATP dynamics better than subtidal species under acute heat stress. Sci Rep 11:11074. |
Willis Jaime R, Hickey Anthony J R, Devaux Jules B L (2021) Sci Rep
Abstract: Temperature is a key factor that affects all levels of organization. Minute shifts away from thermal optima result in detrimental effects that impact growth, reproduction and survival. Metabolic rates of ectotherms are especially sensitive to temperature and for organisms exposed to high acute temperature changes, in particular intertidal species, energetic processes are often negatively impacted. Previous investigations exploring acute heat stress have implicated cardiac mitochondrial function in determining thermal tolerance. The brain, however, is by weight, one of the most metabolically active and arguably the most temperature sensitive organ. It is essentially aerobic and entirely reliant on oxidative phosphorylation to meet energetic demands, and as temperatures rise, mitochondria become less efficient at synthesising the amount of ATP required to meet the increasing demands. This leads to an energetic crisis. Here we used brain homogenate of three closely related triplefin fish species (Bellapiscis medius, Forsterygion lapillum, and Forsterygion varium) and measured respiration and ATP dynamics at three temperatures (15, 25 and 30 °C). We found that the intertidal B. medius and F. lapillum were able to maintain rates of ATP production above rates of ATP hydrolysis at high temperatures, compared to the subtidal F. varium, which showed no difference in rates at 30 °C. These results showed that brain mitochondria became less efficient at temperatures below their respective species thermal limits, and that energetic surplus of ATP synthesis over hydrolysis narrows. In subtidal species synthesis matches hydrolysis, leaving no scope to elevate ATP supply. • Keywords: Physiology, Metabolism, Ecology, Ecophysiology • Bioblast editor: Reiswig R, Plangger M • O2k-Network Lab: NZ Auckland Hickey AJ
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Organism: Fishes
Tissue;cell: Nervous system
Preparation: Homogenate
Regulation: ATP, Temperature Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, NS, ROX HRR: Oxygraph-2k
2021-07, MgG
