Difference between revisions of "Winkler-Stuck 2004 J Neurol Sci"
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{{Publication | {{Publication | ||
|title=Winkler-Stuck K, Wiedemann FR, Wallesch CW, Kunz WS (2004) Effect of coenzyme Q10 on the mitochondrial function of skin fibroblasts from Parkinson patients. J Neurol Sci 220: 41- | |title=Winkler-Stuck K, Wiedemann FR, Wallesch CW, Kunz WS (2004) Effect of coenzyme Q10 on the mitochondrial function of skin fibroblasts from Parkinson patients. J Neurol Sci 220:41-8. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/15140604 PMID: 15140604] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/15140604 PMID: 15140604] | ||
|authors=Winkler-Stuck K, Wiedemann FR, Wallesch CW, Kunz WS | |authors=Winkler-Stuck K, Wiedemann FR, Wallesch CW, Kunz WS | ||
|year=2004 | |year=2004 | ||
|journal=J | |journal=J Neurol Sci | ||
|abstract=Several lines of evidence suggest an impairment of mitochondrial function in the brain of patients with Parkinson's disease (PD). However, the presence of a detectable mitochondrial defect in extracerebral tissue of these patients remains a matter of dispute. Therefore, we investigated mitochondrial function in fibroblasts of 18 PD patients applying biochemical micromethods. Putative beneficial effects of coenzyme Q10 (CoQ10), a potent antioxidant, on the mitochondrial function of skin fibroblast cultures were evaluated. Applying inhibitor titrations of the mitochondrial respiration to calculate flux control coefficients of respiratory chain complexes I and IV, we observed deficiencies of both complexes in cultivated skin fibroblasts of PD patients. Cultivation of fibroblasts in the presence of 5 ฮผM CoQ10ย restored the activity of impaired respiratory chain complexes in the fibroblast cultures of 9 out of 18 PD patients. Our data support the presence of a generalised mitochondrial defect in at least a subgroup of patients with PD that can be partially ameliorated in vitro by coenzyme Q10 treatment. | |abstract=Several lines of evidence suggest an impairment of mitochondrial function in the brain of patients with Parkinson's disease (PD). However, the presence of a detectable mitochondrial defect in extracerebral tissue of these patients remains a matter of dispute. Therefore, we investigated mitochondrial function in fibroblasts of 18 PD patients applying biochemical micromethods. Putative beneficial effects of coenzyme Q10 (CoQ10), a potent antioxidant, on the mitochondrial function of skin fibroblast cultures were evaluated. Applying inhibitor titrations of the mitochondrial respiration to calculate flux control coefficients of respiratory chain complexes I and IV, we observed deficiencies of both complexes in cultivated skin fibroblasts of PD patients. Cultivation of fibroblasts in the presence of 5 ฮผM CoQ10ย restored the activity of impaired respiratory chain complexes in the fibroblast cultures of 9 out of 18 PD patients. Our data support the presence of a generalised mitochondrial defect in at least a subgroup of patients with PD that can be partially ameliorated ''in vitro'' by coenzyme Q10 treatment. | ||
|keywords=Parkinson's disease, Mitochondria, Oxidative phosphorylation, Fibroblasts, Coenzyme Q10 | |keywords=Parkinson's disease, Mitochondria, Oxidative phosphorylation, Fibroblasts, Coenzyme Q10 | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |diseases=Parkinson's | ||
|injuries=Mitochondrial | |injuries=Mitochondrial disease | ||
|organism=Human | |organism=Human | ||
|tissues=Fibroblast | |tissues=Fibroblast | ||
|preparations=Intact | |preparations=Intact cells | ||
|instruments=Oxygraph-2k | |||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
Latest revision as of 15:47, 9 November 2016
| Winkler-Stuck K, Wiedemann FR, Wallesch CW, Kunz WS (2004) Effect of coenzyme Q10 on the mitochondrial function of skin fibroblasts from Parkinson patients. J Neurol Sci 220:41-8. |
Winkler-Stuck K, Wiedemann FR, Wallesch CW, Kunz WS (2004) J Neurol Sci
Abstract: Several lines of evidence suggest an impairment of mitochondrial function in the brain of patients with Parkinson's disease (PD). However, the presence of a detectable mitochondrial defect in extracerebral tissue of these patients remains a matter of dispute. Therefore, we investigated mitochondrial function in fibroblasts of 18 PD patients applying biochemical micromethods. Putative beneficial effects of coenzyme Q10 (CoQ10), a potent antioxidant, on the mitochondrial function of skin fibroblast cultures were evaluated. Applying inhibitor titrations of the mitochondrial respiration to calculate flux control coefficients of respiratory chain complexes I and IV, we observed deficiencies of both complexes in cultivated skin fibroblasts of PD patients. Cultivation of fibroblasts in the presence of 5 ฮผM CoQ10 restored the activity of impaired respiratory chain complexes in the fibroblast cultures of 9 out of 18 PD patients. Our data support the presence of a generalised mitochondrial defect in at least a subgroup of patients with PD that can be partially ameliorated in vitro by coenzyme Q10 treatment. โข Keywords: Parkinson's disease, Mitochondria, Oxidative phosphorylation, Fibroblasts, Coenzyme Q10
Labels:
Pathology: Parkinson's
Stress:Mitochondrial disease
Organism: Human
Tissue;cell: Fibroblast
Preparation: Intact cells
HRR: Oxygraph-2k
