Comparison of respirometric methods

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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Comparison of respirometric methods

Description

The comparison of respirometric methods provides the basis to evaluate different instrumental platforms and different mitochondrial preparations, as a guide to select the best approach and to critically evaluate published results.


 YearReferenceOrganismTissue;cell
MiPNet18.10 O2k-Specifications2019-09-19
Oroboros
O2k-specifications for respirometry and comprehensive OXPHOS analysis.
HumanFibroblast
Perry 2013 Diabetes2013Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53.HumanSkeletal muscle
Zhang 2012 Nat Protoc2012Zhang J, Nuebel E, Wisidagama DR, Setoguchi K, Hong JS, Van Horn CM, Imam SS, Vergnes L, Malone CS, Koehler CM, Teitell MA (2012) Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. Nat Protoc 7: 1068-1085.Stem cells
Horan 2012 J Gerontol A Biol Sci Med Sci2012Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35.
  Discussion


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: O2k-Respirometry