Kiss 2013 FASEB J
| Kiss G, Konrad C, Doczi J, Starkov AA, Kawamata H, Manfredi G, Zhang SF, Gibson GE, Beal MF, Adam-Vizi V, Chinopoulos C (2013) The negative impact of alpha-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation. FASEB J 27:2392-406. |
Kiss G, Konrad C, Doczi J, Starkov AA, Kawamata H, Manfredi G, Zhang SF, Gibson GE, Beal MF, Adam-Vizi V, Chinopoulos C (2013) FASEB J
Abstract: A decline in Ξ±-ketoglutarate dehydrogenase complex (KGDHC) activity has been associated with neurodegeneration. Provision of succinyl-CoA by KGDHC is essential for generation of matrix ATP (or GTP) by substrate-level phosphorylation catalyzed by succinyl-CoA ligase. Here, we demonstrate ATP consumption in respiration-impaired isolated and in situ neuronal somal mitochondria from transgenic mice with a deficiency of either dihydrolipoyl succinyltransferase (DLST) or dihydrolipoyl dehydrogenase (DLD) that exhibit a 20-48 % decrease in KGDHC activity. Import of ATP into the mitochondrial matrix of transgenic mice was attributed to a shift in the reversal potential of the adenine nucleotide translocase toward more negative values due to diminished matrix substrate-level phosphorylation, which causes the translocase to reverse prematurely. Immunoreactivity of all three subunits of succinyl-CoA ligase and maximal enzymatic activity were unaffected in transgenic mice as compared to wild-type littermates. Therefore, decreased matrix substrate-level phosphorylation was due to diminished provision of succinyl-CoA. These results were corroborated further by the finding that mitochondria from wild-type mice respiring on substrates supporting substrate-level phosphorylation exhibited βΌ30 % higher ADP-ATP exchange rates compared to those obtained from DLST+/- or DLD+/- littermates. We propose that KGDHC-associated pathologies are a consequence of the inability of respiration-impaired mitochondria to rely on "in-house" mitochondrial ATP reserves. β’ Keywords: Adenine nucleotide translocase; Reversal potential; Succinyl-CoA ligase, F0βF1 ATP synthase
β’ O2k-Network Lab: HU Budapest Chinopoulos C
Cited by
- KomlΓ³di et al (2022) The protonmotive force - not merely membrane potential. MitoFit Preprints 2022 (in prep)
- KomlΓ³di et al (2022) The protonmotive force - not merely membrane potential. MitoFit Preprints 2022 (in prep)
Labels: MiParea: Respiration, Genetic knockout;overexpression Pathology: Neurodegenerative
Organism: Mouse Tissue;cell: Nervous system
Enzyme: TCA cycle and matrix dehydrogenases Regulation: ADP, ATP, Ion;substrate transport Coupling state: OXPHOS Pathway: N HRR: Oxygraph-2k
MitoFit2022rTCA, MitoFit 2022 pmF
