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Chinopoulos Christos

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


Chinopoulos Christos


MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1


COST Mentor: Mentor

MC Member - Management Committee MitoEAGLE
Name Chinopoulos Christos, MD, PhD, Associate Professor
Institution
Christos Chinopoulos

Department of Medical Biochemistry,

Semmelweis University, HU

Address Tuzolto st. 37-47, 1094
City Budapest
State/Province
Country Hungary
Email [email protected]
Weblink Christos Chinopoulos
O2k-Network Lab HU Budapest Chinopoulos C

Bioenergetics Communications

Keywords: mitochondria, oncometabolism, tumor metabolism, substrate-level phosphorylation
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NextGen-O2k

  • Prof. Christos Chinopoulos is Key Opinion Leader (KOL) in the NextGen-O2k project.

MitoEAGLE Short-Term Scientific Mission

Work Plan summary
In our laboratory we examine the contribution of mitochondrial substrate-level phosphorylation (mSLP) to ATP output and ANT directionality during true anoxia or inhibition of the electron transport chain (ETC) in isolated mitochondria or permeabilized cells. True anoxia is ensured by monitoring oxygen concentration in sealed Oroboros chambers when mitochondria respire on various substrates in the abundance of ADP. On the other hand, chemical anoxia is induced by targeted inhibition of ETC components. Under such conditions mSLP is addressed by protocols relying on membrane potential measurements using safranine O, also monitored by the O2k-Fluo LED2-Module, as described elsewhere (FASEB J. 2010, 24:2405). However, it has come to our attention that targeted inhibition of complex I perturbs mSLP even in the presence of true anoxia. Parallel to these measurements, metabolomic analysis of citricacid cycle metabolites obtained from fractions of such experiments hint on a privileged dependence of complex I activity to KGDHC. Currently, we are attempting to address this further by measuring NADH in standard fluorimetric cuvettes using home-made 3D-printed plugs in order to induce and maintain anoxia where we can also measure membrane potential by safranine O among other parameters. However, we are only partially successful, and we need to be able to measure oxygen, NADH and safranine O fluorescence in the same samples (albeit in different chambers), simultaneously. The O2k-NextGen is designed by Oroboros for achieving exactly that. The plan is to isolate mouse liver mitochondria, subject them to ADP and substrates in sealed Oroboros chambers, attain anoxia, add the ETC inhibitors and collect fractions at various time points to be evaluated by untargeted metabolomic analysis. Anoxia, NADH levels and membrane potential will be measured throughout these experiments and complement the results of the metabolomic analysis.


Participated at


Visiting scientist in the Oroboros O2k-Laboratory

O2k-Network

Christos Chinopoulos : Visiting scientist at the Oroboros O2k-Laboratory

  • December 14 to December 22 2017


Labels: Field of research: Basic  Topics: Mitochondrial mechanisms of neurodegeneration, Permeability transition pore 


Publications

Abstracts

 PublishedReference
Pallag 2022 Abstract Bioblast2022P06.
Pallag Gergely
Pallag Gergely, Nazarian S, Ravasz D, Bui D, Komlรณdi T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation supports mitochondrial ATP production when Complex I is inhibited. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001
ยปMitoFit Preprintยซ
Ravasz 2019 Abstract IOC1412019Ravasz D, Bui D, Kitayev A, Greenwood B, Hill C, Komlodi T, Doerrier C, Ozohanics O, Moore AL, Gnaiger E, Kiebish M, Kolev K, Seyfried TN, Willis WT, Narain N, Adam-Vizi V, Chinopoulos C (2019) Endogenous quinones sustain a moderate NADH oxidation by Complex I during anoxia. Mitochondr Physiol Network 24.02.
Chinopoulos 2018 MiP20182018
Christos Chinopoulos
Exclusive neuronal expression of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation.
Komlodi 2018b EBEC20182018Endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.
Ravasz 2018 Abstract The evolving concept of mitochondria2018Vast pools of endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.
Chinopoulos 2012 Abstract Bioblast2012Dobolyi A, Ostergaard E, Bago AG, Palkovits M, Adam-Vizi V, Chinopoulos C (2012) Exclusive neuronal expression of SUCLA2 in the human brain. Mitochondr Physiol Network 17.12.
Kiss 2012 Abstract Bioblast2012Kiss G, Konrad C, Doczi J, Starkov AA, Kawamata H, Manfredi G, Zhang SF, Gibson GE, Beal MF, Adam-Vizi V, Chinopoulos C (2012) The negative impact of alpha-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation. Mitochondr Physiol Network 17.12.
Konrad 2012 Abstract Bioblast2012Konrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12.
Chinopoulos C 2012 Abstract Bioblast2012Doczi J, Aniko Gal A, Echaniz-Laguna A, Mousson de Camaret B, Molnar MJ, Adam-Vizi V Chinopoulos C (2012) Properties of the mitochondrial permeability transition in human cells lacking the adenine nucleotide translocase isoform 1. Mitochondr Physiol Network 17.12.