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Went 2021 MitoFit PB

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Went 2021 MitoFit PB

Publications in the MiPMap
Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005

» MitoFit Preprints 2021.05.

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Went Nora, Di Marcello Marco, Gnaiger Erich (2021) MitoFit Prep

Abstract: Gnaiger 2022 Abstract Bioblast-PB: The bioenergetic crosstalk between mitochondria and chloroplasts plays a key role in maintaining metabolic integrity and controlling metabolite production for growth and regulation of cell concentration. Dark respiration and photosynthesis were measured in the green alga Chlamydomonas reinhardtii at varying oxygen concentrations and three cell concentrations using the NextGen-O2k with the PhotoBiology Module. Maximum net photosynthesis at a light intensity of 350 µmol∙s-1∙m-2 (blue light) was inhibited at hyperoxia by 40 % at oxygen concentrations of 550 to 650 µM. Light-enhanced dark respiration reached a (negative) maximum within 30 to 60 s after light-dark transitions and was 3.5- to 4-fold higher than steady-state dark respiration independent of O2 concentration in the range of 200 to 650 µM.

Keywords: high-resolution respirometry, photosynthesis, dark respiration, Chlamydomonas reinhardtii Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Oroboros

Data availability and further experimental details

Original files are available Open Access at Zenodo repository: https://zenodo.org/record/4729616#.Ys6clYRBy60
» Went N (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. Batchelor thesis:26 pp. - »Bioblast link«


link:https://youtu.be/YdlSqOEwi5Y • Podcast

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References

LinkReferenceYearView
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_32018PMID: 29850993 »O2k-brief
O2k-Protocols
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-00022020Open Access pdf published online 2020-12-30

Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v12020Bioenerg Commun 2020.1. Open Access pdf published online 2020-05-20

Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.00012020MitoFit Preprint Arch EA20.1.
MitoFit pdf
High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations
Shimakawa G, Kohara A, Miyake C (2020) Characterization of light-enhanced respiration in cyanobacteria. Int J Mol Sci 22:342. https://doi.org/10.3390/ijms220103422020PMID: 33396191 Open Access
Xue X, Gauthier DA, Turpin DH, Weger HG (1996) Interactions between photosynthesis and respiration in the green alga Chlamydomonas reinhardtii. Characterization of light-enhanced dark respiration. Plant Physiol 112:1005–14. https://doi.org/10.1104/pp.112.3.10051996Open Access

Event

» Presented at ISAP2021

Support

Template NextGen-O2k.jpg
Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.

O2k-Publications

» O2k-Publications: Chlamydomonas


Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP 


Organism: Algae 

Preparation: Intact cells 

Regulation: Oxygen kinetics  Coupling state: ROUTINE 

HRR: Oxygraph-2k, NextGen-O2k, O2k-Protocol 

Bioblast 2022, Chlamydomonas, LEDR, Photosynthesis, O2k-Demo, Plant physiology