- high-resolution terminology - matching measurements at high-resolution
Additive effect of convergent electron flow
Description
[[Description::There is an additive effect on respiration by electron flow converging at the Q-junction from respiratory Complexes I and II (CI+II e-input), glycerophosphate dehydrogenase and electron-transferring flavoprotein. Convergent electron flow corresponds to the operation of the TCA cycle and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the Q-junction supports higher OXPHOS capacity and ETS capacity than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular TCA cycle function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with mt-preparations have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to additivity.]]
Abbreviation: A
Reference: [[Info::MiPNet12.12, Gnaiger_2009_IJBCB]]
MitoPedia methods:
Respirometry
Labels:
Preparation: Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate
Enzyme: Complex I, Complex II; Succinate Dehydrogenase
Regulation: Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity
HRR: Theory