From Bioblast
Description
Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent Ξ²-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
Abbreviation: Car
Application in HRR
- Car: L-carnitine: Sigma Aldrich; C0158; FW=161.2
- Preparation of 250 mM stock solution (dissolved in H2O):
- Weigh 100 mg of Carnitine.
- Add 2.48 mL H2O.
- Divide into 100 Β΅L portions.
- Store frozen at -20Β°C.
- O2k Manual titration
- Titration volume: 4 Β΅L 250 mM stock using a 10 Β΅L Hamilton syringe (2 mL O2k-Chamber).
- Final concentration: 0.5 mM.
- O2k Manual titration
Feeding carnitine
MitoPedia topics:
Substrate and metabolite