Difference between revisions of "Citrate synthase"
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::::# Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6. - [[Gnaiger 2019 MitoFit Preprint Arch |Ā»Bioblast linkĀ«]] | ::::# Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6. - [[Gnaiger 2019 MitoFit Preprint Arch |Ā»Bioblast linkĀ«]] | ||
::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1- | ::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - [[MiPNet17.04 CitrateSynthase |Ā»Bioblast linkĀ«]] | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Enzyme | |mitopedia topic=Enzyme | ||
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Revision as of 19:47, 19 April 2020
Description
Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.
Abbreviation: CS
Reference: MiPNet17.04 CitrateSynthase
Normalization of respiratory flux for CS as a mitochondrial marker
Communicated by Gnaiger Erich last update 2020-04-19
- Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, JO2/CS [pmolāsĀ-1āIUĀ-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
- Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmolās-Ā1āmLĀ-1āIUĀ-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2].
- Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6. - Ā»Bioblast linkĀ«
- Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - Ā»Bioblast linkĀ«
MitoPedia topics:
Enzyme