Citrate synthase: Difference between revisions
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:::: Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, ''e.g.'' [pmol∙s<sup>-1</sup>∙mL<sup>-1</sup>∙IU<sup>-1</sup>], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2]. | :::: Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, ''e.g.'' [pmol∙s<sup>-1</sup>∙mL<sup>-1</sup>∙IU<sup>-1</sup>], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2]. | ||
::::# Gnaiger | ::::# Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - [[BEC2020.1 doi10.26124bec2020-0001.v1 |»Bioblast link«]] | ||
::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - [[MiPNet17.04 CitrateSynthase |»Bioblast link«]] | ::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - [[MiPNet17.04 CitrateSynthase |»Bioblast link«]] | ||
Revision as of 08:48, 23 May 2020
Description
Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.
Abbreviation: CS
Reference: MiPNet17.04 CitrateSynthase
Normalization of respiratory flux for CS as a mitochondrial marker
Communicated by Gnaiger Erich last update 2020-04-19
- Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, JO2/CS [pmol∙s-1∙IU-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
- Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmol∙s-1∙mL-1∙IU-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2].
- Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - »Bioblast link«
- Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - »Bioblast link«
MitoPedia topics:
Enzyme