Difference between revisions of "Flux control ratio"
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== Flux control factor: normalization of mitochondrial respiration == | == Flux control factor: normalization of mitochondrial respiration == | ||
* ''More details:'' [[Flux control factor]] | * ''More details:'' [[Flux control factor]] |
Revision as of 14:19, 8 February 2016
Description
Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.
Abbreviation: FCR
Reference: Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol, MiPNet19.18E O2 Flux Analysis
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab
Flux control factor: normalization of mitochondrial respiration
- More details: Flux control factor
DatLab
The entire plot of oxygen flux can be transformed to a FCR. Click on 'Slope' in the pull-down menu. - MiPNet19.18E O2 Flux Analysis.