Flux control ratio: Difference between revisions
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|description='''Flux control ratios''' (''FCR''), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%). ย | |description='''Flux control ratios''' (''FCR''), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%). ย | ||
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). ''FCR'' obtained from a single respirometric incubation (sequential protocol; [[SUIT|SUIT protocol]]) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. ''FCR'' obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]]. | For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). ''FCR'' obtained from a single respirometric incubation with sequential titrations (sequential protocol; [[SUIT|SUIT protocol]]) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. ''FCR'' obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]], [[Pesta 2012 Methods Mol Biol]], [[MiPNet19.18E O2 flux analysis]] | |info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger 2009 Int J Biochem Cell Biol]], [[Pesta 2012 Methods Mol Biol]], [[MiPNet19.18E O2 flux analysis]] | ||
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== DatLab == | == DatLab == | ||
:::: The entire plot of oxygen flux can be transformed to a FCR. Click on 'Slope' in the pull-down menu. - [[MiPNet19.18E O2 flux analysis]]. | :::: The entire plot of oxygen flux can be transformed to a ''FCR''. Click on 'Flux/Slope' in the pull-down menu. - [[MiPNet19.18E O2 flux analysis]]. |
Revision as of 12:35, 14 September 2016
Description
Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation with sequential titrations (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.
Abbreviation: FCR
Reference: Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Pesta 2012 Methods Mol Biol, MiPNet19.18E O2 flux analysis
MitoPedia concepts:
Respiratory control ratio,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab
Flux control factor: normalization of mitochondrial respiration
- ยป More details: Flux control factor
DatLab
- The entire plot of oxygen flux can be transformed to a FCR. Click on 'Flux/Slope' in the pull-down menu. - MiPNet19.18E O2 flux analysis.