GM-pathway control state: Difference between revisions
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|description=[[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]]. | |description=[[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]]. | ||
'''MitoPathway control state:''' [[ | '''MitoPathway control state:''' [[NADH electron transfer-pathway state]] | ||
The '''GM-pathway control state''' (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase. | The '''GM-pathway control state''' (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase. |
Revision as of 11:20, 2 August 2023
Description
MitoPathway control state: NADH electron transfer-pathway state
The GM-pathway control state (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of oxaloacetate, comparable to the metabolism with acetyl-CoA and citrate synthase.
Abbreviation: GM
Reference: Gnaiger 2020 BEC MitoPathways
- More details
GML
GMP
GME
- GM pathway in the ET state can be evaluated in the following SUIT protocols:
- DL-Protocol for permeabilized cells (pce):
Discussion
- In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GMP) is identical or 10 % higher than with PM (PMP). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PMP being 16% to 25% higher than for GMP. In fibroblasts, GMP supports a higher respiratory flux than PMP.
MitoPedia concepts:
SUIT state