Difference between revisions of "IOC61"
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[[File:O2k-TIP2k.jpg|right|300px|caption]] | |||
'''O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced''' | |||
Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011. | |||
Hotel Körbersee | |||
For some pictures, see [[IOC60_Photos|IOC60]] | |||
More information on IOC61: http://www.oroboros.at/index.php?nextcourse | |||
'''Contact''': [[Has mailaddress::{{{mailaddress|[email protected]}}}]] | |||
==Course structure== | |||
* Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages. | |||
* Hot Topics in Mitochondrial Physiology: 10+5 min | |||
==Abstracts== | |||
*[[:IOC61_Abstracts|Look at Abstracts >>]] | |||
*[http://www.bioblast.at/index.php/Special:AbstractsExtension?event=IOC61 Add your Abstract >>] | |||
For the abstract format, see [[Author_IOC61]]. | |||
== Combined introductory and advanced O2k-Workshop with parallel sessions == | |||
::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:''' | |||
'''A1. General [[HRR]] topics''' | |||
* O2k-assembly, | |||
* [[Polarographic oxygen sensor]] (OroboPOS) service, | |||
* POS calibration, | |||
* [[DatLab 4.3|DatLab]] software: principles and operation, | |||
* [[Titration-Injection-microPump]] - TIP2k. | |||
'''A2. Protocols in HRR''' | |||
* Introduction to fundamental elements of mitochondrial respiratory physiology, | |||
* Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria. | |||
'''A3. Hands-on experiments with the O2k''' | |||
* Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control, | |||
* Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]], | |||
* Preparation of [[permeabilized muscle fibre]]s, | |||
* HRR with permeabilized muscle fibres. | |||
'''A4. Presentation and interpretation of [[oxygen flux]]''' | |||
* Normalization of respiration per tissue or per [[mitochondrial marker]], | |||
* Coupling control ratios, | |||
* Substrate control ratios, | |||
* Generalized flux control ratios. | |||
'''A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:''' | |||
* Mitochondrial membrane potential (TPP+ electrode), | |||
* Acidification (pH electrode), | |||
* Nitric oxide (amperometric), | |||
* Spectrophotometry (cytochrome spectra), | |||
* Spectrofluorimetry (Amplex red, safranin, etc.) | |||
::'''B: Advanced users will join in small working teams in parallel sessions, with specific topics: | |||
''' | |||
'''B1. O2k-MultiSensor and TIP2k''' | |||
* Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]] | |||
* Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis, | |||
* ADP kinetics: experimental examples with TIP2-steady state control and data analysis, | |||
* pH-stat feedback control, | |||
'''B2. Theoretical perspectives''' | |||
* Advanced experimental protocols, | |||
* Quality control - towards [[quality assurance]]; | |||
* ''Add your topic'': | |||
==Participants and areas of interest== | |||
* [[Alexeyev M|'''Alexeyev Mikhail, USA''']]: Mouse modeling of mitochondrial disorders caused by mtDNA mutations. | |||
* [[Angelin A|'''Angelin Alessia, USA''']]: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy). | |||
* [[Balboa Castillo E|'''Balboa Castillo Elisa, Chile''']]: Increase in mitochondrial cholesterol and dysfunction in NPC. | |||
* [[Castelein N|'''Castelein Natascha, Belgium''']]: The role of mitochondria in dietary restriction induced lifespan extension in ''C. elegan''s. | |||
* [[Gabrielova E|'''Gabrielova Eva, Czech Republic''']]: [[Gabrielova IOC61|Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.]] | |||
* [[Hals I|'''Hals Ingrid, Norway''']]: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions. | |||
* [[Hancock S|'''Hancock Saege, USA''']] | |||
* [[Kasal D|'''Kasal Daniel, Brazil''']]: Modulation of cardiomyocyte respiration by bone marrow stem cells. | |||
* [[Labieniec M|'''Labieniec Magdalena, Poland''']]: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS | |||
* [[Le C|'''Le Catherine, USA''']]: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential. | |||
* [[Magnifico MC|'''Magnifico Maria Chiara, Italy''']]: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells. | |||
* [[Mandl M|'''Mandl Markus, Vienna''']] | |||
* [[Manjeri G|'''Manjeri Ganesh, The Netherlands''']]: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts. | |||
* [[Marelsson S|'''Marelsson Sigurdur, Sweden''']]: The role of mitochondrial dysfunction in encephalopathy. | |||
* [[Morein T|'''Morein Torbjörn, Sweden''']] | |||
* [[Nuskova H|'''Nuskova Hana, Czech Republic''']]: [[Nuskova IOC61|Mitochondrial Energetics in Health and Disease]]. | |||
* [[Sanz A|'''Sanz Alberto, Finland''']]: Mechanism causing aging and the role of mitochondria in this process. | |||
* [[Scherer Alves L|'''Scherer Alves Leonardo, USA''']]: Mitochondrial Physiology as a force of human adaptation and evolution to different geographic niches and the relation between the environment and mitochondrial genes in human common diseases. | |||
* [[Watala C|'''Watala Cezary, Poland''']]: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS | |||
* [[Yokota T|'''Yokota Takashi, Denmark''']]: The role of mitochondria in chronic heart failure, diabetes, or obesity. | |||
* [[Zurmanova J|'''Zurmanova Jitka, Czech Republic''']]: Mechanisms of regulation of OXPHOS by local ADP in muscle cells. | |||
== Tutors == | |||
* [[Gnaiger E|'''Erich Gnaiger''']] (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT) | |||
* [[Fasching M|'''Mario Fasching''']] (OROBOROS INSTRUMENTS, AT) | |||
* [[Pesta D|'''Dominik Pesta''']] (Innsbruck Medical University, AT) | |||
* [[Sumbalova Z|'''Zuzana Sumbalova''']] (OROBOROS INSTRUMENTS, AT) | |||
==Discussion== | |||
[[Talk:IOC61|Continue the Discussion >>]] | |||
[[Category:IOC61]] | |||
[[Category:OroboroPedia]] | |||
[[Category:Events]] |
Revision as of 12:33, 5 April 2011
O2k-Workshop on High-Resolution Respirometry - Introductory and Advanced
Schröcken, Vorarlberg, Austria 26 April - 1 May, 2011.
Hotel Körbersee
For some pictures, see IOC60
More information on IOC61: http://www.oroboros.at/index.php?nextcourse
Contact: [email protected]
Course structure
- Introductory and advanced groups will be working in parallel session, to adjust the programme optimally to the specific needs of the participants in different workpackages.
- Hot Topics in Mitochondrial Physiology: 10+5 min
Abstracts
For the abstract format, see Author_IOC61.
Combined introductory and advanced O2k-Workshop with parallel sessions
- A: Introductory lectures and hands-on experiments for basic HRR in small working teams:
A1. General HRR topics
- O2k-assembly,
- Polarographic oxygen sensor (OroboPOS) service,
- POS calibration,
- DatLab software: principles and operation,
- Titration-Injection-microPump - TIP2k.
A2. Protocols in HRR
- Introduction to fundamental elements of mitochondrial respiratory physiology,
- Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
A3. Hands-on experiments with the O2k
- Measurement of instrumental background oxygen flux, manual and TIP2k-feedback control,
- Intact cells: Respirometric reference assay with yeast, phosphorylation control protocol,
- Preparation of permeabilized muscle fibres,
- HRR with permeabilized muscle fibres.
A4. Presentation and interpretation of oxygen flux
- Normalization of respiration per tissue or per mitochondrial marker,
- Coupling control ratios,
- Substrate control ratios,
- Generalized flux control ratios.
A5. Overview on O2k-MultiSensor applications - Simultaneous measurement of respiration and:
- Mitochondrial membrane potential (TPP+ electrode),
- Acidification (pH electrode),
- Nitric oxide (amperometric),
- Spectrophotometry (cytochrome spectra),
- Spectrofluorimetry (Amplex red, safranin, etc.)
- B: Advanced users will join in small working teams in parallel sessions, with specific topics:
B1. O2k-MultiSensor and TIP2k
- Hands-on MultiSensor TPP+ applications, see TPP special interest group during an International Oxygraph Course
- Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
- ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
- pH-stat feedback control,
B2. Theoretical perspectives
- Advanced experimental protocols,
- Quality control - towards quality assurance;
- Add your topic:
Participants and areas of interest
- Alexeyev Mikhail, USA: Mouse modeling of mitochondrial disorders caused by mtDNA mutations.
- Angelin Alessia, USA: Function in muscle tissue; in particular human muscle disorder (muscular dystrophy).
- Balboa Castillo Elisa, Chile: Increase in mitochondrial cholesterol and dysfunction in NPC.
- Castelein Natascha, Belgium: The role of mitochondria in dietary restriction induced lifespan extension in C. elegans.
- Gabrielova Eva, Czech Republic: Evaluation of oxygen consumption in rat heart mitochondria treatment by polyphenol compounds.
- Hals Ingrid, Norway: Effects of hypoxia in pancreatic beta-cells on mitochondrial functions.
- Hancock Saege, USA
- Kasal Daniel, Brazil: Modulation of cardiomyocyte respiration by bone marrow stem cells.
- Labieniec Magdalena, Poland: Mitochondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
- Le Catherine, USA: Implications of mitochondrial dysfunction in heart disease; cardiomypathy; ROS; mitochondrial function, respiration, membrane potential.
- Magnifico Maria Chiara, Italy: Measurement of oxygen consumption, respiratory flux and membrane potential in HaCaT cells.
- Mandl Markus, Vienna
- Manjeri Ganesh, The Netherlands: Understanding oxygen flux in mitochondrial disorders working with skin fibroblasts.
- Marelsson Sigurdur, Sweden: The role of mitochondrial dysfunction in encephalopathy.
- Morein Torbjörn, Sweden
- Nuskova Hana, Czech Republic: Mitochondrial Energetics in Health and Disease.
- Sanz Alberto, Finland: Mechanism causing aging and the role of mitochondria in this process.
- Scherer Alves Leonardo, USA: Mitochondrial Physiology as a force of human adaptation and evolution to different geographic niches and the relation between the environment and mitochondrial genes in human common diseases.
- Watala Cezary, Poland: Mitocondrial respiratory capacity; mt membrane potential; calcium homeostasis; mitochondria in health and pathology (diabetes, neurodegenerative diseases, cancer); mt function; ROS
- Yokota Takashi, Denmark: The role of mitochondria in chronic heart failure, diabetes, or obesity.
- Zurmanova Jitka, Czech Republic: Mechanisms of regulation of OXPHOS by local ADP in muscle cells.
Tutors
- Erich Gnaiger (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
- Mario Fasching (OROBOROS INSTRUMENTS, AT)
- Dominik Pesta (Innsbruck Medical University, AT)
- Zuzana Sumbalova (OROBOROS INSTRUMENTS, AT)