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MiP03

From Bioblast


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MiP03

Description

Mitochondrial Preservation Medium, MiP03, developed for preservation of isolated mitochondria.

Abbreviation: MiP03

Reference: MiPNet14.13 Medium-MiR06; Gnaiger 2000 Life in the Cold


MitoPedia methods: Respirometry 


MitoPedia topics: Media for respirometry 


  • For preparation of MiP03 see MiPNet14.13 Medium-MiR06.
  • (+)-ฮฑ-Tocopherol acid succinate (T3126, Sigma Aldrich): dissolve 1.0616 mg in 1 ml Ethanol (100%) and store aliquots at 4 ยฐC (dark glass vials)
  • Leupeptine (L9783, Sigma Aldrich): dissolve 0.463 mg in 1 ml H2O and store frozen aliquots at -20 ยฐC