Difference between revisions of "MiPNet23.01 IOC126 Innsbruck AT"
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== General information == | == General information == | ||
::::The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric ( | ::::The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic. | ||
[[File:IOC126.png|center|500px|data analysis]] | [[File:IOC126.png|center|500px|data analysis]] | ||
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|11:00 | |11:00 | ||
|Determination of mitochondrial membrane potential (mtMP) with potentiometric | |Determination of mitochondrial membrane potential (mtMP) with potentiometric O2k-TPP+ ISE-Module - an introduction. | ||
|MiP''Art'' | |MiP''Art'' | ||
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|13:15 | |13:15 | ||
|Hands-on: Assembly and maintenance of TPP and reference | |Hands-on: Assembly and maintenance of TPP+ and reference electrodes [[MiPNet15.03 O2k-MultiSensor-ISE]] | ||
| | |MitoFit ORO Lab | ||
|- | |- | ||
|14:30 | |14:30 | ||
|Hands-on: Set up of the instruments with TPP and reference electrodes + | |Hands-on: Set up of the instruments with TPP+ and reference electrodes, TPP+ calibration [[MiPNet15.03 O2k-MultiSensor-ISE]] | ||
|MitoFit ORO Lab | |MitoFit ORO Lab | ||
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|17:00 | |17:00 | ||
|Hands-on continued: TPP chemical background [[MiPNet14.05 TPP-mtMembranePotential]] | |Hands-on continued: TPP+ chemical background [[MiPNet14.05 TPP-mtMembranePotential]] | ||
| | |MitoFit ORO Lab | ||
|} | |} | ||
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|09:00 | |09:00 | ||
|Introduction in planning and performing a TPP experiment | |Introduction in planning and performing a TPP+ experiment. | ||
| | |MitoFit ORO Lab | ||
|- | |- | ||
|09:30 | |09:30 | ||
|Hands-on: Calibration of | |Hands-on: Calibration of TPP+ electrode. | ||
|MitoFit ORO Lab | |MitoFit ORO Lab | ||
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|11:00 | |11:00 | ||
|Hands-on continued TPP: experiment with mouse brain mitochondria | |Hands-on continued TPP+: experiment with mouse brain mitochondria. | ||
|MitoFit ORO Lab | |MitoFit ORO Lab | ||
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|14:00 | |14:00 | ||
| From the TPP signal to mt-membrane potential - Guide through the Excel templates. [[Media:MiPNet14.05_App_TPP-Mathematics-MembranePotential.pdf|TPP-mtMembranePotential]] mathematics | | From the TPP signal to mt-membrane potential - Guide through the Excel templates. [[Media:MiPNet14.05_App_TPP-Mathematics-MembranePotential.pdf|TPP-mtMembranePotential]] mathematics | ||
| | |MitoFit ORO Lab | ||
|- | |- | ||
|14:30 | |14:30 | ||
| Analysis of TPP experiment | | Analysis of TPP experiment and discussion on mitochondrial membrane potential. | ||
| | |MitoFit ORO Lab | ||
|- | |- | ||
|16:00 | |16:00 | ||
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|17:00 | |17:00 | ||
|Determination of mitochondrial membrane potential (mtMP) with | |Determination of mitochondrial membrane potential (mtMP) with fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module) - an introduction. | ||
|MitoFit ORO Lab | |MitoFit ORO Lab | ||
|- | |- | ||
|17:30 | |17:30 | ||
|Hands-on: Set up of the instruments with LED2-Module, [[MiPNet17.05 O2k-Fluo LED2-Module]], | |Hands-on: Set up of the instruments with LED2-Module, choosing Fluorescence Sensors [[MiPNet17.05 O2k-Fluo LED2-Module]], [http://wiki.oroboros.at/index.php/Fluorescence-Sensor] | ||
Calibration of Safranin/TMRM, chemical background experiment | Calibration of Safranin/TMRM, chemical background experiment | ||
|MitoFit ORO Lab | |MitoFit ORO Lab | ||
| Line 134: | Line 134: | ||
|09:00 | |09:00 | ||
|Introduction in planning and performing an experiment with Safranin/TMRM | |Introduction in planning and performing an experiment with Safranin/TMRM | ||
| | |MitoFit ORO Lab | ||
|- | |- | ||
| | |10:00 | ||
|Hands-on: O2k setup for air calibration | |Hands-on: O2k setup for air calibration | ||
|MitoFit ORO Lab | |MitoFit ORO Lab | ||
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|14:00 | |14:00 | ||
|Analysis of TMRM/Safranin experiment - discussion | |Analysis of TMRM/Safranin experiment - discussion | ||
| | |MitoFit ORO Lab | ||
|- | |- | ||
|15: | |15:30 | ||
|''Coffee/Tea'' | |''Coffee/Tea'' | ||
| | | | ||
|- | |- | ||
|16: | |16:00 | ||
| | |Comparison of TPP+ and fluorescence methods for evaluation of mitochondrial membrane potential. | ||
|MiP''Art'' | |MiP''Art'' | ||
|- | |- | ||
|17:00 | |17:00 | ||
|Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications | |Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications | ||
|the Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions | |||
|MiP''Art'' | |MiP''Art'' | ||
|- | |- | ||
Revision as of 14:17, 20 December 2017
MiPNet23.01 IOC126 Innsbruck AT
 Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126. |
Oroboros (2018-01-24) Mitochondr Physiol Network
Abstract:
Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
Venue
- Information on travel and venue:IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
- Mention "Oroboros" when booking in order to receive a discount.
Preliminary programme
Wednesday 24th Topic Room 09:00 Get-together: Introduction of participants and their research interests MiPArt 09:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. See: Abstract. MiPArt 10:00 O2k-MultiSensor overview, DatLab 7, DatLab 7 Protocols MiPArt 10:30 Coffee/Tea 11:00 Determination of mitochondrial membrane potential (mtMP) with potentiometric O2k-TPP+ ISE-Module - an introduction. MiPArt 11:45 Lunch break 13:15 Hands-on: Assembly and maintenance of TPP+ and reference electrodes MiPNet15.03 O2k-MultiSensor-ISE MitoFit ORO Lab 14:30 Hands-on: Set up of the instruments with TPP+ and reference electrodes, TPP+ calibration MiPNet15.03 O2k-MultiSensor-ISE MitoFit ORO Lab 16:30 Coffee/Tea 17:00 Hands-on continued: TPP+ chemical background MiPNet14.05 TPP-mtMembranePotential MitoFit ORO Lab
Thursday 25th Topic Room 09:00 Introduction in planning and performing a TPP+ experiment. MitoFit ORO Lab 09:30 Hands-on: Calibration of TPP+ electrode. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on continued TPP+: experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 From the TPP signal to mt-membrane potential - Guide through the Excel templates. TPP-mtMembranePotential mathematics MitoFit ORO Lab 14:30 Analysis of TPP experiment and discussion on mitochondrial membrane potential. MitoFit ORO Lab 16:00 Coffee/Tea 16:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab 17:00 Determination of mitochondrial membrane potential (mtMP) with fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module) - an introduction. MitoFit ORO Lab 17:30 Hands-on: Set up of the instruments with LED2-Module, choosing Fluorescence Sensors MiPNet17.05 O2k-Fluo LED2-Module, [1] Calibration of Safranin/TMRM, chemical background experiment
MitoFit ORO Lab
Friday 26th Topic Room 09:00 Introduction in planning and performing an experiment with Safranin/TMRM MitoFit ORO Lab 10:00 Hands-on: O2k setup for air calibration MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on: Calibration of Safranin/TMRM, experiment with mouse brain mitochondria/HEK cells MitoFit ORO Lab 12:30 Lunch break 14:00 Analysis of TMRM/Safranin experiment - discussion MitoFit ORO Lab 15:30 Coffee/Tea 16:00 Comparison of TPP+ and fluorescence methods for evaluation of mitochondrial membrane potential. MiPArt 17:00 Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications the Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions MiPArt 18:00 Farewell activity
Registration
Tutors and Lecturers
Tutors
Sumbalova Zuzana, PhD, Medical University of Innsbruck - Principal investigator
Chang Shao-Chiang MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRACT
Passrugger Manuela, BSc., Oroboros Instruments - Biomedical Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRANSMIT
Lecturers and assistance
Doerrier Carolina,PhD, Oroboros Instruments - Chief Scientific Officer
Komlodi Timea,Dr., Oroboros Instruments - Scientific Researcher
Meszaros Andras, MD, PhD, Oroboros Instruments - Postdoctoral researcher
List of participants
- Preliminary list
| Participant | Institution | |
|---|---|---|
| Kandel Sunil Mani | US WI Milwaukee Dash RK - Medical College of Wisconsin | |
| Smirnova Irina | SE Stockholm Smirnova I - Stockholm University | |
|
Labieniec-Watala Magdalena | PL Lodz Watala C - University of Lodz |
| Janssen Connie | NL Leiden Lindeman JH - Leiden University Medical Center | |
| de Kok Michéle | NL Leiden Lindeman JH - Leiden University Medical Center | |
| Steffen Anja | NL Leiden Lindeman JH - Leiden University Medical Center | |
|
Siewiera Karolina | PL Lodz Watala C - University of Lodz |
|
Michalak Slawomir | PL Poznan Michalak S - Poznan University of Medical Sciences |
| Fernandez-Vizarra Erika | UK Cambridge Zeviani M - University of Cambridge |
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Labels:
ORO, IOC, MitoFit, Next, 2018
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
