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Difference between revisions of "MiPNet23.01 IOC126 Innsbruck AT"

From Bioblast
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== General information ==
== General information ==
::::The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (TPP+/O2k-TPP+ ISE-Module) and amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) Furthermore, data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.  
::::The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (TPP+/O2k-TPP+ ISE-Module) and amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) Furthermore, data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.  
[[File:IOC126.png|center|500px|data analysis]]


== Venue ==
== Venue ==
Line 23: Line 25:


== Programme ==
== Programme ==
in progress
{| class='wikitable'
:::: The programme of the O2k-Workshop TPP/ISE and HRFR is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of mitochondrial membrane potential (TPP+, TPMP+) as well as with the O2k-FluoRespirometer.
|-
|style="width: 10em"|'''Wednesday 24th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Get-together: Introduction of participants and their research interests
|MiP''Art''
|-
|09:30
|O2k-MultiSensor overview, DatLab7, DatLab7 Protocols
|MiP''Art''
|-
|10:00
|''Coffee/Tea''
|
|-
|10:30
|Determination of mitochondrial (mt) membrane potential with potentiometric TPP+/O2k-TPP+ ISE-Module - Introduction
|MiP''Art''
|-
|11:15
|Hands-on: Assembly and maintenance of TPP and reference electrode O2k-MultiSensor-Manual MiPNet15.03
|MiP''Art''
|-
|12:30
|''Lunch break ''
|
|-
|14:00
|Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration O2k-MultiSensor-Manual MiPNet15.03
|MitoFit ORO Lab
|-
|12:30
|''Coffee/Tea''
|
|-
|16:30
|Hands-on continue TPP chemical background MiPNet14.05
|MiP''Art''
|-
|}
 
{| class='wikitable'
|-
|style="width: 10em"|'''Thursday 25th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Introduction in Planning and Performing a TPP experiment; Instrumental background oxygen flux in the presence of the TPP electrode O2k-Background MiPNet14.06 Instrumental O2 background
|MiP''Art''
|-
|09:30
|Hands-on: Calibration of TPP electrode
|MitoFit ORO Lab
|-
|10:30
|''Coffee/Tea''
|
|-
|11:00
|Hands-on continue TPP: experiment with mouse brain mitochondria
|MitoFit ORO Lab
|-
|12:30
|''Lunch break''
|
|-
|14:00
| From the TPP signal to mt-membrane potential - Guide through the Excel templates. MiPNet14.05 mathematics
|MiP''Art''
|-
|14:30
| Analysis of TPP experiment - discussion on Mitochondrial membrane potential, MiPNet14.05 mathematics
|MiP''Art''
|-
|16:00
|''Coffee/Tea''
|
|-
|16:30
|Hands-on: O2k setup for air calibration
|MitoFit ORO Lab
|-
|17:00
|Determination of mitochondrial (mt) membrane potential with amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) - introduction
|MitoFit ORO Lab
|-
|17:30
|Hands-on: Set up of the instruments with LED2-Module, LED2-Module MiPNet17.05,
Calibration of Safranin/TMRM, chemical background experiment
|MitoFit ORO Lab
|-
|}
 
{| class='wikitable'
|-
|style="width: 10em"|'''Friday 26th'''
|style="width: 60em"|'''Topic'''
|style="width: 15em"|'''Room'''
|-
|09:00
|Introduction in Planning and Performing an experiment with Safranin/TMRM
|MiP''Art''
|-
|09:30
|Hands-on: O2k setup for air calibration
|MitoFit ORO Lab
|-
|10:30
|''Coffee'/Tea''
|
|-
|11:00
|Hands-on: Calibration of Safranin/TMRM, experiment with HEK cells
|MitoFit ORO Lab
|-
|12:30
|''Lunch break''
|
|-
|14:00
|Analysis of TMRM/Safranin experiment - discussion
|MiP''Art''
|-
|15:00
|''Coffee/Tea''
|
|-
|16:30
|Round table: questions
|MiP''Art''
|-
|17:00
|Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications,  The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions
|MiP''Art''
|-
|18:00
|''Farewell activity''
|
|-
|}
 
 
 


== Registration ==
== Registration ==

Revision as of 16:37, 11 December 2017

                



MiPNet23.01 IOC126 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126.


Oroboros (2018-01-24) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.


O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


General information

The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (TPP+/O2k-TPP+ ISE-Module) and amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) Furthermore, data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
data analysis


Venue

Information on travel and venue:IOC Innsbruck.
» Hotel suggestion: Hotel Weisses Kreuz
Mention "Oroboros" when booking in order to receive a discount.

Programme

Wednesday 24th Topic Room
09:00 Get-together: Introduction of participants and their research interests MiPArt
09:30 O2k-MultiSensor overview, DatLab7, DatLab7 Protocols MiPArt
10:00 Coffee/Tea
10:30 Determination of mitochondrial (mt) membrane potential with potentiometric TPP+/O2k-TPP+ ISE-Module - Introduction MiPArt
11:15 Hands-on: Assembly and maintenance of TPP and reference electrode O2k-MultiSensor-Manual MiPNet15.03 MiPArt
12:30 Lunch break
14:00 Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration O2k-MultiSensor-Manual MiPNet15.03 MitoFit ORO Lab
12:30 Coffee/Tea
16:30 Hands-on continue TPP chemical background MiPNet14.05 MiPArt
Thursday 25th Topic Room
09:00 Introduction in Planning and Performing a TPP experiment; Instrumental background oxygen flux in the presence of the TPP electrode O2k-Background MiPNet14.06 Instrumental O2 background MiPArt
09:30 Hands-on: Calibration of TPP electrode MitoFit ORO Lab
10:30 Coffee/Tea
11:00 Hands-on continue TPP: experiment with mouse brain mitochondria MitoFit ORO Lab
12:30 Lunch break
14:00 From the TPP signal to mt-membrane potential - Guide through the Excel templates. MiPNet14.05 mathematics MiPArt
14:30 Analysis of TPP experiment - discussion on Mitochondrial membrane potential, MiPNet14.05 mathematics MiPArt
16:00 Coffee/Tea
16:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab
17:00 Determination of mitochondrial (mt) membrane potential with amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) - introduction MitoFit ORO Lab
17:30 Hands-on: Set up of the instruments with LED2-Module, LED2-Module MiPNet17.05,

Calibration of Safranin/TMRM, chemical background experiment

MitoFit ORO Lab
Friday 26th Topic Room
09:00 Introduction in Planning and Performing an experiment with Safranin/TMRM MiPArt
09:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab
10:30 Coffee'/Tea
11:00 Hands-on: Calibration of Safranin/TMRM, experiment with HEK cells MitoFit ORO Lab
12:30 Lunch break
14:00 Analysis of TMRM/Safranin experiment - discussion MiPArt
15:00 Coffee/Tea
16:30 Round table: questions MiPArt
17:00 Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications, The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions MiPArt
18:00 Farewell activity



Registration

Registration form

Tutors and Lecturers

Tutors

Lecturers and assistance

List of participants

Participant Institution
Kandel Sunil Mani US WI Milwaukee Dash RK - Medical College of Wisconsin
Smirnova Irina SE Stockholm Smirnova I - Stockholm University
Magdalena Labieniec-Watala Labieniec-Watala Magdalena PL Lodz Watala C - University of Lodz
Janssen Connie NL Leiden Lindeman JH - Leiden University Medical Center
de Kok Michéle NL Leiden Lindeman JH - Leiden University Medical Center
Steffen Anja NL Leiden Lindeman JH - Leiden University Medical Center
Siewiera Karolina Siewiera Karolina PL Lodz Watala C - University of Lodz
Michalak Slawomir PL Poznan Michalak S - Poznan University of Medical Sciences
Fernandez-Vizarra Erika UK Cambridge Zeviani M - University of Cambridge


Contact

[email protected]
Oroboros Instruments
High-Resolution FluoRespirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png


Labels:






ORO, IOC, MitoFit, Next, 2018 


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.