MiPNet23.01 IOC126 Innsbruck AT
 Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126. |
Oroboros (2018-01-24) Mitochondr Physiol Network
Abstract:
Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (TPP+/O2k-TPP+ ISE-Module) and amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) Furthermore, data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
Venue
- Information on travel and venue:IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
- Mention "Oroboros" when booking in order to receive a discount.
Programme
Wednesday 24th Topic Room 09:00 Get-together: Introduction of participants and their research interests MiPArt 09:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. See: Abstract. MiPArt 10:00 O2k-MultiSensor overview, DatLab7, DatLab7 Protocols MiPArt 10:30 Coffee/Tea 11:00 Determination of mitochondrial (mt) membrane potential with potentiometric TPP+/O2k-TPP+ ISE-Module - Introduction MiPArt 11:45 Lunch break 13:15 Hands-on: Assembly and maintenance of TPP and reference electrode O2k-MultiSensor-Manual MiPNet15.03 MiPArt 14:30 Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration O2k-MultiSensor-Manual MiPNet15.03 MitoFit ORO Lab 16:30 Coffee/Tea 17:00 Hands-on continued: TPP chemical background MiPNet14.05 MiPArt
Thursday 25th Topic Room 09:00 Introduction in Planning and Performing a TPP experiment; Instrumental background oxygen flux in the presence of the TPP electrode O2k-Background MiPNet14.06 Instrumental O2 background MiPArt 09:30 Hands-on: Calibration of TPP electrode MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on continue TPP: experiment with mouse brain mitochondria MitoFit ORO Lab 12:30 Lunch break 14:00 From the TPP signal to mt-membrane potential - Guide through the Excel templates. MiPNet14.05 mathematics MiPArt 14:30 Analysis of TPP experiment - discussion on Mitochondrial membrane potential, MiPNet14.05 mathematics MiPArt 16:00 Coffee/Tea 16:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab 17:00 Determination of mitochondrial (mt) membrane potential with amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) - introduction MitoFit ORO Lab 17:30 Hands-on: Set up of the instruments with LED2-Module, LED2-Module MiPNet17.05, Calibration of Safranin/TMRM, chemical background experiment
MitoFit ORO Lab
Friday 26th Topic Room 09:00 Introduction in planning and performing an experiment with Safranin/TMRM MiPArt 09:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on: Calibration of Safranin/TMRM, experiment with mouse brain mitochondria/HEK cells MitoFit ORO Lab 12:30 Lunch break 14:00 Analysis of TMRM/Safranin experiment - discussion MiPArt 15:00 Coffee/Tea 16:30 Round table: questions MiPArt 17:00 Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications, The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions MiPArt 18:00 Farewell activity
Registration
Tutors and Lecturers
Tutors
Sumbalova Zuzana, PhD, Medical University of Innsbruck - Principal investigator
Chang Shao-Chiang MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRACT
Passrugger Manuela, BSc., Oroboros Instruments - Biomedical Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRANSMIT
Lecturers and assistance
Doerrier Carolina,PhD, Oroboros Instruments - Chief Scientific Officer
Komlodi Timea,Dr., Oroboros Instruments - Scientific Researcher
Meszaros Andras, MD, PhD, Oroboros Instruments - Postdoctoral researcher
List of participants
- Preliminary list
| Participant | Institution | |
|---|---|---|
| Kandel Sunil Mani | US WI Milwaukee Dash RK - Medical College of Wisconsin | |
| Smirnova Irina | SE Stockholm Smirnova I - Stockholm University | |
|
Labieniec-Watala Magdalena | PL Lodz Watala C - University of Lodz |
| Janssen Connie | NL Leiden Lindeman JH - Leiden University Medical Center | |
| de Kok Michéle | NL Leiden Lindeman JH - Leiden University Medical Center | |
| Steffen Anja | NL Leiden Lindeman JH - Leiden University Medical Center | |
|
Siewiera Karolina | PL Lodz Watala C - University of Lodz |
|
Michalak Slawomir | PL Poznan Michalak S - Poznan University of Medical Sciences |
| Fernandez-Vizarra Erika | UK Cambridge Zeviani M - University of Cambridge |
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Labels:
ORO, IOC, MitoFit, Next, 2018
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
