Difference between revisions of "MiPNet23.02 IOC127 Szeged HU"
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== General information == Â | == General information == Â | ||
:::: This O2k-Workshop takes place satellite to the [[WISe3_2018_Szeged_HU| WISe3 Conference in Szeged, HU]]. Separate registration is required for this workshop. Â | :::: This O2k-Workshop takes place satellite to the [[WISe3_2018_Szeged_HU| WISe3 Conference in Szeged, HU]]. Separate registration is required for this workshop. Â | ||
::::'''[http://wiki.oroboros.at/images/7/7f/WISe3_Flyer.pdf Programme overview]''' | |||
::::'''[http://wiki.oroboros.at/images/8/8d/WISe3_Program.pdf Detailed programme]''' | |||
::::'''[http://wiki.oroboros.at/images/4/40/WISe3_abstract_book.pdf Abstract book]''' | |||
:::: Three topics will be covered during the one-day workshop: | :::: Three topics will be covered during the one-day workshop: | ||
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== Registration == | == Registration == | ||
:::: The registration is free of charge, but we ask you for informal registration by Mar 19 at latest. Â | :::: The registration is free of charge, but we ask you for informal registration by Mar 19 at latest. Â | ||
:::: The number of participants will be limited. Please let us know by email if you will attend: [email protected] | :::: The number of participants will be limited. Please let us know by email if you will attend: [mailto:[email protected]| [email protected]] | ||
  |   | ||
== Tutors and Lecturers == | == Tutors and Lecturers == | ||
<br/> | <br/> | ||
<gallery mode=default perrow=6 widths="140px" heights="150px"> | <gallery mode=default perrow=6 widths="140px" heights="150px"> | ||
File:Gnaiger Erich.jpg|[[Gnaiger Erich]], CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory | File:Gnaiger Erich.jpg|'''[[Gnaiger Erich]]''', CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory | ||
File:MeszarosA.JPG|'''[[Meszaros A|AndrĂĄs MĂ©szĂĄros]]''', MD., PhD., Chief Research Officer, Oroboros Instruments and Assistant Professor, University of Szeged, Institute of Surgical Research | File:MeszarosA.JPG|'''[[Meszaros A|AndrĂĄs MĂ©szĂĄros]]''', MD., PhD., Chief Research Officer, Oroboros Instruments and Assistant Professor, University of Szeged, Institute of Surgical Research | ||
File:KomlodiT.JPG|'''[[Komlodi T|Timea KomlĂČdi]]''', Scientific assistant, Oroboros Instruments | File:KomlodiT.JPG|'''[[Komlodi T|Timea KomlĂČdi]]''', Scientific assistant, Oroboros Instruments | ||
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| 09:00 | | 09:00 | ||
| | | | ||
|'''Morning session on | |'''Morning session on mitochondria''' | ||
| | |||
|- | |||
| 09:00 | |||
| [[Image:PecinaP.JPG|75px|Petr Pecina]] | |||
| [[Pecina P |Petr Pecina]] | |||
'''Changes in oxygen kinetics between COX4i1 and COX4i2-containing COX''' | |||
| | |||
|- | |||
| 09:30 | |||
| [[Image:Tretter_L.JPG|75px|Laszlo Tretter]] | |||
| [[Tretter L |Laszlo Tretter]] | |||
'''Bioenergetical approach of neurodegenerative diseases''' | |||
| | |||
|- | |||
| 10:00 | |||
| [[Image:Juhasz_L.jpg|75px|Laszlo Juhasz]] | |||
| [[Juhasz L |Laszlo Juhasz]] | |||
Ca<sup>(2+)</sup>N it be measured? High-resolution O2k-FluoRespirometric detection of extramitochondrial calcium movement | |||
| | |||
|- | |||
| 10:10 | |||
| | |||
| David Kurszan Jasz | |||
Mitochondrial effects of methane gas treatment on rat cardiomyocytes subjected to simulated ischemia/reperfusion | |||
| | |||
|- | |||
| 10:20 | |||
| | |||
| Eszter Tuboly | |||
Novel cardioprotective effects of methane in an ex vivo model | |||
| | |||
|- | |||
| | |||
| Â | | Â | ||
|- | |- | ||
| 10: | | 10:30 | ||
| | | | ||
| ''Coffee/tea break'' | | ''Coffee/tea break'' | ||
|- | |- | ||
| | | 10:45 | ||
| [[Image:MeszarosA.JPG|75px|Andras Meszaros]] Â | | [[Image:MeszarosA.JPG|75px|Andras Meszaros]] Â | ||
| [[Meszaros A |Andras Meszaros]] | | [[Meszaros A |Andras Meszaros]] | ||
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| Â | | Â | ||
|- | |- | ||
| 12: | | 12:15 | ||
| | | | ||
| ''Lunch break'' | | ''Lunch break'' | ||
|- | |||
| 13:00 | |||
| [[Image:Christos_Chinopoulos.jpg|75px|Chinopoulos Christos]] | |||
| [[Chinopoulos C|Chinopoulos Christos]] | |||
'''Interplay of respiratory components and mitochondrial diaphorases on redox state in anoxia''' | |||
|- | |- | ||
| 13:30 | | 13:30 | ||
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| [[Komlodi T |Timea Komlodi]] | | [[Komlodi T |Timea Komlodi]] | ||
'''Oxygen dependence of H<sub>2</sub>O<sub>2</sub> production:''' an application of | '''Oxygen dependence of H<sub>2</sub>O<sub>2</sub> production:''' an application of high-resolution respirometry with Amplex UltraRed(R) fluorescence. | ||
| [[Hydrogen peroxide]] | | [[Hydrogen peroxide]] | ||
|- | |- | ||
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| 16:30 | | 16:30 | ||
| | | | ||
| | |'''COST Action MitoEAGLE session''' | ||
| | |||
|- | |||
| 16:30 | |||
| | |||
| [[Ledo AM |Ledo Ana Margarida]] | |||
Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimerâs disease: implications on mitochondrial function | |||
| | |||
|- | |||
| 16:45 | |||
| [[Image:KrakoN.jpg|75px|Nina Krako]] | |||
| [[Krako Jakovljevic N |Krako JakovljeviÄ Nina]] | |||
Investigation of subcellular mechanisms in insulin resistance models in hepatocytes and myocytes | |||
| | |||
|- | |||
| 17:00 | |||
| [[Image:VelikaBeata.JPG|75px|Beata Cizmarova ]] | |||
| [[Cizmarova B |Beata Cizmarova ]] | |||
The effect of short-term exopsure to moderate altitude on respiration of peripheral-blood mononuclear cells | |||
| | |||
|- | |||
| 17:15 | |||
| | |||
| '''Poster session and discussions''' | |||
|- | |- | ||
| | |||
| | |||
| [[Evinova A |Andrea Evinova]] | |||
Magnesium protects from calcium induced collapse of mitochondrial trans-membrane potential. | |||
|- | |||
| | |||
| [[File:Kasja picture.JPG|75px|Kasja Pavlovic]] | |||
| [[Pavlovic K |Kasja Pavlovic]] | |||
C2C12 myoblasts as a cell model for studying the role of mitochondria in insulin resistance | |||
|- | |||
| | |||
| | |||
| [[Pavel I |Ioana Pavel]] | |||
The acute and chronic effects of a benzylamide derivative of maslinic acid in liver mitochondria isolated from mice with chemically induced skin carcinogenesis | |||
|} | |} | ||
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== List of participants== | == List of participants== | ||
 | {| class="wikitable" | ||
|- | |||
! | |||
! Participant | |||
! Institution | |||
|- | |||
| | |||
| Bori Zoltan | |||
| University of Physical Education, Budapest | |||
|- | |||
| | |||
| [[Budai A|Budai Andras]] | |||
| HU Budapest Szijjarto A - Semmelweis University | |||
|- | |||
| [[File:Christos_Chinopoulos.jpg|100px|Chinopoulos Christos]] | |||
| [[Chinopoulos C|Chinopoulos Christos]] | |||
| [[HU Budapest Chinopoulos C]] - Semmelweis University | |||
|- | |||
| [[File:KolonicsA.jpg|100px|Kolonics Attila]] | |||
| [[Kolonics A|Kolonics Attila]] | |||
| BioFirmware Ltd | |||
|- | |||
| | |||
| Koltai Erika | |||
| University of Physical Education, Budapest | |||
|- | |||
| | |||
| [[Pavel I|Pavel Ioana]] | |||
| [[RO Timisoara Muntean DM]] - Victor Babes University of Medicine and Pharmacy | |||
|- | |||
| [[File:PecinaP.JPG|100px|Pecina Petr]] | |||
| [[Pecina P|Pecina Petr]] | |||
| [[CZ Prague Houstek J]] - Academy of Sciences of the Czech Republic | |||
|- | |||
| | |||
| Talas David | |||
| University of Szeged | |||
|- | |||
| | |||
| [[Sergeeva T|Sergeeva Tatiana]] | |||
| [[RU Nizhny Novgorod Mukhina I]] - Nizhny Novgorod State Medical Academy  | |||
|- | |||
| [[File:Tretter_L.JPG|100px|Tretter Laszlo]] | |||
| [[Tretter L|Tretter Laszlo]] | |||
| [[HU Budapest Tretter L]] - Semmelweis University | |||
|- | |||
| | |||
| Windedal Tobias | |||
| | |||
|- | |||
|} | |||
 | |||
== WISe3 Conference Szeged == | == WISe3 Conference Szeged == | ||
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:::: Oroboros Instruments | :::: Oroboros Instruments | ||
:::: High- | :::: High-resolution respirometry | ||
:::: Schöpfstrasse 18 | :::: Schöpfstrasse 18 | ||
:::: A-6020 Innsbruck, Austria | :::: A-6020 Innsbruck, Austria | ||
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{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=ORO, IOC, O2k-Network, 2018 | |additional=ORO, IOC, O2k-Network, 2018 | ||
}} | }} | ||
Latest revision as of 00:00, 18 March 2019
MiPNet23.02 IOC127 Szeged HU
Szeged HU, 2018 Mar 27. Oroboros satellite O2k-Workshop on hypoxia IOC127. |
Oroboros (2018-03-27) Mitochondr Physiol Network
Abstract:
Oroboros O2k-Workshop on hypoxia in cooperation with HU Szeged Boros M. Szeged, Hungary, 2018 March 27.
âą O2k-Network Lab: AT_Innsbruck_OROBOROS, HU Szeged Boros M
General information
- This O2k-Workshop takes place satellite to the WISe3 Conference in Szeged, HU. Separate registration is required for this workshop.
- Three topics will be covered during the one-day workshop:
- (1) Andras Meszaros: Closed-chamber mitochondrial oxygen kinetics with demo experiment and introduction of the data analysis module O2kinetics - new development.
- (2) Erich Gnaiger: Assessment of mitochondrial and cellular oxygen kinetics by steady-state oxygen titrations, using the Titration-Injection microPump TIP2k,
- (3) Timea Komlodi: Measurement of H2O2 production rate with the O2k-FluoRespirometer and Amplex UltraRed(R) fluorescence. Dependence of ROS production on oxygen concentration.
- Three topics will be covered during the one-day workshop:
Venue
- Clinical Skills Centre, Faculty of Medicine
- University of Szeged
- 13 Dom square, HU-6720
- Szeged, Hungary
- HU Szeged Boros M
Local organizers
Prof. Boros Mihaly, HU Szeged Boros M
Univ-Doz. Dr. Andrea Szabo, HU Szeged Boros M
Registration
- The registration is free of charge, but we ask you for informal registration by Mar 19 at latest.
- The number of participants will be limited. Please let us know by email if you will attend: [email protected]
Tutors and Lecturers
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
AndrĂĄs MĂ©szĂĄros, MD., PhD., Chief Research Officer, Oroboros Instruments and Assistant Professor, University of Szeged, Institute of Surgical Research
Timea KomlĂČdi, Scientific assistant, Oroboros Instruments
Programme
Tuesday Mar 27
Time | Topic | Materials | |
---|---|---|---|
09:00 | Morning session on mitochondria | ||
09:00 | Petr Pecina
Changes in oxygen kinetics between COX4i1 and COX4i2-containing COX |
||
09:30 | Laszlo Tretter
Bioenergetical approach of neurodegenerative diseases |
||
10:00 | Laszlo Juhasz
Ca(2+)N it be measured? High-resolution O2k-FluoRespirometric detection of extramitochondrial calcium movement |
||
10:10 | David Kurszan Jasz
Mitochondrial effects of methane gas treatment on rat cardiomyocytes subjected to simulated ischemia/reperfusion |
||
10:20 | Eszter Tuboly
Novel cardioprotective effects of methane in an ex vivo model |
||
10:30 | Coffee/tea break | ||
10:45 | Andras Meszaros
Mitochondrial oxygen kinetics in closed-chamber respirometers - Introduction. |
Gnaiger 1995 J Bioenerg Biomembr | |
Mitochondrial oxygen kinetics in closed-chamber respirometers - O2k demo experiment. |
|||
Mitochondrial oxygen kinetics in closed-chamber respirometers - Data analysis. Introduction of the new DatLab analysis module O2kinetics |
|||
12:15 | Lunch break | ||
13:00 | Chinopoulos Christos
Interplay of respiratory components and mitochondrial diaphorases on redox state in anoxia | ||
13:30 | Timea Komlodi
Oxygen dependence of H2O2 production: an application of high-resolution respirometry with Amplex UltraRed(R) fluorescence. |
Hydrogen peroxide | |
Oxygen dependence of H2O2 production: Data analysis | Hydrogen peroxide | ||
14:30 | Coffee/tea break | ||
14:45 | Erich Gnaiger
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - A comparison of steady-state and closed-chamber mitochondrial oxygen kinetics. |
TIP2k-Module | |
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Programming the TIP2k. |
TIP2k-Module | ||
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Calculations and data analysis. Theoretical background. |
Harrison 2015 J Appl Physiol | ||
16:15 | Coffee/tea break | ||
16:30 | COST Action MitoEAGLE session | ||
16:30 | Ledo Ana Margarida
Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimerâs disease: implications on mitochondrial function |
||
16:45 | Krako JakovljeviÄ Nina
Investigation of subcellular mechanisms in insulin resistance models in hepatocytes and myocytes |
||
17:00 | Beata Cizmarova
The effect of short-term exopsure to moderate altitude on respiration of peripheral-blood mononuclear cells |
||
17:15 | Poster session and discussions | ||
Andrea Evinova
Magnesium protects from calcium induced collapse of mitochondrial trans-membrane potential. | |||
Kasja Pavlovic
C2C12 myoblasts as a cell model for studying the role of mitochondria in insulin resistance | |||
Ioana Pavel
The acute and chronic effects of a benzylamide derivative of maslinic acid in liver mitochondria isolated from mice with chemically induced skin carcinogenesis |
List of participants
Participant | Institution | |
---|---|---|
Bori Zoltan | University of Physical Education, Budapest | |
Budai Andras | HU Budapest Szijjarto A - Semmelweis University | |
Chinopoulos Christos | HU Budapest Chinopoulos C - Semmelweis University | |
Kolonics Attila | BioFirmware Ltd | |
Koltai Erika | University of Physical Education, Budapest | |
Pavel Ioana | RO Timisoara Muntean DM - Victor Babes University of Medicine and Pharmacy | |
Pecina Petr | CZ Prague Houstek J - Academy of Sciences of the Czech Republic | |
Talas David | University of Szeged | |
Sergeeva Tatiana | RU Nizhny Novgorod Mukhina I - Nizhny Novgorod State Medical Academy | |
Tretter Laszlo | HU Budapest Tretter L - Semmelweis University | |
Windedal Tobias |
WISe3 Conference Szeged
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
- Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«
- Isolated mitochondria and intact cells.
- Nanomolar resolution of oxygen concentration.
- Effects of cell density and activity.
- Closed versus open systems.
- p50 in vivo.
- Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«
- Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
- A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen
- Mitochondrial kinetics measured by high-resolution respirometry
- Calibrations and corrections for response time and instrumental background
- Steady-state injection respirometry
- Mitochondrial respiratory control at low oxygen
- Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
- Effect of ADP and oxygen limitation on ADP/O2 flux ratios
- The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
- Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
- Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«
- First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph.
- Standardized oxygen calibration (air and internal zero oxygen calibration).
- Automatic instrumental background correction of oxygen flux.
- Deconvolution of the oxygen signal for time resolution required in oxygen kinetics.
- Quality control of the limit of detection of oxygen flux at ±1 pmol O2·s-1·ml-1.
- Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2018
O2k-Workshops are listed as MitoGlobal Events.'