MiPNet25.03 IOC144 Innsbruck AT: Difference between revisions
No edit summary |
No edit summary |
||
Line 28: | Line 28: | ||
== Registration == | == Registration == | ||
<br> | <br> | ||
:::: Download registration form, fill in the pdf and the print to sign: [https://wiki.oroboros.at/images/4/46/Registration_form_IOC143.pdf download pdf] | :::: Download registration form, fill in the pdf and the print to sign: [https://wiki.oroboros.at/images/4/46/Registration_form_IOC143.pdf download pdf] ย ย ย | ||
<br> | |||
== Tutors and lecturers == | == Tutors and lecturers == | ||
<br/> | <br/> | ||
<gallery mode=default perrow=5 widths="140px" heights="150px"> | <gallery mode=default perrow=5 widths="140px" heights="150px"> |
Revision as of 16:45, 28 October 2019
MiPNet25.03 IOC144 Innsbruck AT
Innsbruck AT, 2020 Jan 20-22. MitoFit Coaching Days - Basic IOC143. |
Oroboros (2020-01-20) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.
โข O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- MitoFit Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols adn will be shown at the Coaching Days.
Venue
- ยป Oroboros MitoFit Laboratory
- Schoepfstrasse 18, 6020 Innsbruck
- ยป Information on travel and venue: IOC Innsbruck.
- ยป Oroboros MitoFit Laboratory
Registration
- Download registration form, fill in the pdf and the print to sign: download pdf
Tutors and lecturers
Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Scientific Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Preliminary programme
Monday 20th
Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests ORO-MitoFit Lab 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab 09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab 10:10 Hands-on: DatLab 7 ORO-MitoFit Lab 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
ORO-MitoFit Lab 13:30 Instrumental quality control 2: Instrumental O2 background โ overview with videoclips ORO-MitoFit Lab 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 21st
Topic Room 09:00 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 09:15 Application of the O2k Series H ORO-MitoFit Lab 09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols โ fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:30 Introduction to DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
|}
Wednesday 22nd
Topic Room 09:00 MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 10:00 Coffee 10:30 MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:15 DatLab analysis ORO-MitoFit Lab 14:00 DatLab analysis summary ORO-MitoFit Lab 15:00 Coffee 15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt 16:15 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Participant | Institution | |
---|---|---|
Griffiths Kate | UK Glasgow Selman C - Glasgow University, Glasgow (UK) |
Contact
- Oroboros Instruments GmbH
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- E-Mail: [email protected]
- Mitochondria and Cell Research
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoGlobal, Next, 2020