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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
WG1 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Australia | Queensland | Fisher Joshua J |
Australia | Queensland | Holland Olivia J |
Australia | Nedlands | Perks Kara L |
Australia | Melobourne | Botella Javier |
Australia | Melbourne | Stepto Nigel K |
Australia | Southport | Vidimce Josif |
Australia | Newtown, Sydney | Stocker Roland |
Australia | Geelong, Victoria | McKenzie Matthew |
Australia | Melbourne | Whitfield Jamie |
Australia | Burwood | Trewin Adam J |
Australia | Queensland | Dong Lan-Feng |
Australia | Melbourne | Ferri Alessandra |
Australia | Melbourne, VIC | Muccini Anna Maria |
Australia | Victoria | Van Bergen Nicole J |
Australia | Parkville | Hardee Justin P |
Australia | Syndey | Ton Riccardo |
Australia | Melbourne | Kuang Jujiao |
Australia | Melbourne | Hewakapuge Sudinna |
Australia | Victoria | Winwood-Smith Hugh |
Australia | Melbourne | Lionett S |
Australia | Nedlands | Filipovska Aleksandra |
Australia | Clayton Victoria | Alton Lesley |
Australia | Melbourne | Cassar Samantha |
Australia | Melbourne | Saner Nicholas |
Australia | Melbourne | Coughlan Melinda T |
Australia | Sydney | Hoehn Kyle L |
Australia | Queensland | Radenkovic Filip |
Australia | Melbourne | Genders Amanda J |
Australia | Sydney | Krycer James R |
Australia | Clayton | Wolff Jonci Nikolai |
Austria | Innsbruck | Cecatto Cristiane |
Austria | Innsbruck | Keller Markus A |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Thapa Maheshwor |
Austria | Innsbruck | Plangger Mario |
Austria | Innsbruck | Schartner Melanie |
Austria | Innsbruck | Laner Verena |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Di Marcello Marco |
Austria | Innsbruck | Bergmeister Lisa |
Austria | Kapferer Werner | |
Austria | Innsbruck | Antunes Diana |
Austria | Innsbruck | Passrugger Manuela |
Austria | Salzburg | Kofler B |
Austria | Vienna | Kozlov Andrey V |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Perez Valencia Juan Alberto |
Austria | Innsbruck | Gallee Leon |
Austria | Innsbruck | Schwarzer Christoph |
Austria | Aldrans | Haider Markus |
Austria | Klosterneuburg | Sazanov Leonid A |
Austria | Innsbruck | Burtscher Martin |
Austria | Innsbruck | Goebel G |
Austria | Innsbruck | Nindl Veronika |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Klocker Helmut |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Sobotka Ondrej |
Austria | Innsbruck | Schmitt Sabine |
Belgium | Leuven, Flanders | Baekelandt V |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Arnould Thierry |
Belgium | Brussels | Sonveaux P |
Belguim | Sart Tilman (Liège) | Kruse C |
Brasil | Porto Alegre | Haas Clarissa |
Brasilia | Limeira | Ropelle Eduardo R |
Brazil | São Paulo | Kowaltowski Alicia J |
Brazil | São Paulo | Crispim Marcell |
Brazil | Jaboticabal | Oliveira Marcos Tulio |
Brazil | Rio de Janeiro | Vieyra Adalberto |
Brazil | Rio de Janeiro | Oliveira Marcus F |
Brazil | Santa Maria - RS | Goncalves Debora Farina |
Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
Brazil | Sao Paulo | Vercesi Anibal E |
Brazil | Sao Paulo | Silber Ariel Mariano |
Brazil | Rio de Janeiro | Galina Antonio |
Canada | Rimouski | Munro Daniel |
Canada | Edmonton | Lucchinetti Eliana |
Canada | Hamilton | Tarnopolsky Mark A |
Canada | Edmonton | Han Woo Hyun |
Canada | Vancouver, BC | Tausan Daniel |
Canada | Montréal | Breton Sophie |
Canada | Guelph | Holloway Graham P |
Canada | Winnipeg | Chowdhury Subir Roy |
Canada | Quebec | Joseph Vincent |
Canada | Hamilton | Scott Graham R |
Canada | Ottawa | Darveau Charles-A |
Canada | Charlottetown | Adiele Reginald C |
Canada | Ottawa | Harper Mary-Ellen |
Canada | Montreal, Quebec | Bergdahl Andreas |
Canada | Rimouski | Blier Pierre U |
Canada | Antigonish | Weir M |
Canada | Saint John | Pulinilkunnil Thomas |
Canada | Vancouver | Bovard Josh |
Canada | Moncton, NB | Pichaud Nicolas |
Canada | Edmonton (Alberta) | Lemieux Helene |
Canada | Edmonton | Zaugg Michael |
Chile | Coyhaique | Jana Prado Fabian |
China | Xi`an | Liu Jiankang |
China | Pukou, Nanjing, | Gan Zhenji |
China | Tianjin | Zhang Yong |
China | Fuzhou, Fujian Province | Chen Qi |
Croatia | Zagreb | Rubelj Ivica |
Croatia | Split | Ljubkovic M |
Croatia | Zagreb | Saric A |
Croatia | Zagreb | Curik I |
Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
Czech Republic | Pardubice | Majtnerova Pavlina |
Czech Republic | Hradec Kralove | Stankova Pavla |
Czech Republic | Ceske Budejovice | Zikova Alena |
Czech Republic | Hradec Kralove | Cervinkova Zuzana |
Czech Republic | Pardubice | Handl Jiri |
Czech Republic | Praha 2 | Danhelovska Tereza |
Czech Republic | Pardubice | Rousar Tomas |
Czech Republic | Prague | Drahota Zdenek |
Czech Republic | Vestec | Ezrova Zuzana |
Czech Republic | Prague | Krizova J |
Czech Republic | Praha 2 | Zdrazilova Lucie |
Czech Republic | Prague | Houstek Josef |
Czech Republic | Hradec Kralove | Endlicher Rene |
Czech Republic | Vestec | Davidova Eliska |
Czech Republic | Prague 10 | Urban Tomas |
Czech Republic | Hradec Kralove | Kucera Otto |
Czech Republic | Vestec | Rohlena Jakub |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Copenhagen N | Scheibye-Knudsen Morten |
Denmark | Roskilde | Dalgaard Louise T |
Denmark | Copenhagen | Gonzalez-Franquesa Alba |
Denmark | Copenhagen | Kraunsoee R |
Denmark | Copenhagen | Dela Flemming |
Denmark | Copenhagen | Nielsen B |
Denmark | Aarhus N | Boetker Hans Erik |
Denmark | Copenhagen N | Maise Chroeis K |
Denmark | Copenhagen | Larsen Steen |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Soendergaard SD |
Denmark | Aarhus N | Jespersen Nichlas Riise |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tallinn | Kaambre Tuuli |
Estonia | Tallinn | Vendelin Marko |
Estonia | Tallinn | Puurand Marju |
Estonia | Tallinn | Tepp Kersti |
Estonia | Tallinn | Laasmaa Martin |
Estonia | Tallinn | Karro N |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Klepinin Aleksandr |
Estonia | Tartu | Paju Kalju |
Estonia | Tallinn | Shevchuk Igor |
Finland | Helsinki | Suomalainen Wartiovaara Anu |
Finland | Jyvaeskylae | Kainulainen Heikki |
Finland | Turku | Stier Antoine |
Finland | Helsinki | Jackson Christopher Benjamin |
France | Paris | Bouillaud Frederic |
France | Gueguen Naig | |
France | Bordeaux | Sarlak Saharnaz |
France | Angers | Spinazzi Marco |
France | Montpellier | Wrutniak-Cabello Chantal |
France | Angers | Procaccio Vincent |
France | Paris | Armand Anne-Sophie |
France | Bordeaux cedex | Mourier A |
France | Maisons-Alfort Cedex | Prola Alexandre |
France | Bordeaux cedex | Rossignol Rodrigue |
France | Nouzilly | Collin-Chenot Anne |
France | Lille | Montaigne David |
France | Martinique | Neviere Remi |
France | Martinique | Cano Sanchez Maria Consolacion |
France | Plouzané | Salin Karine |
France | Bordeaux | Amoedo Nivea Dias |
France | Tours | Dumas Jean-Francois |
Germany | Cologne | Trifunovic Aleksandra |
Germany | Marburg | Ramzan Rabia |
Germany | Duesseldorf | Jelenik T |
Germany | Freiburg | Schuele R |
Germany | Cologne | Ho Dieu Hien |
Germany | Rostock | Sokolova Inna |
Germany | Giessen | Schulz Rainer |
Germany | Duesseldorf | Haendeler Judith |
Germany | Cologne | Pesta Dominik |
Germany | Ulm | Schaefer PM |
Germany | Bremerhaven | Mark Felix Christopher |
Germany | Cologne | Maciej Sarah |
Germany | Frankfurt | Osiewacz Heinz D |
Germany | Hannover | Das Anibh Martin |
Germany | Jena | Szibor Marten |
Germany | Duesseldorf | Piel Sarah |
Germany | Neuherberg | Einer C |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Biberach an der Riß | Nold V |
Germany | Tuebingen | Kappler Lisa |
Germany | Gießen | Grewal Rekha |
Germany | Frankfurt | Warnsmann Verena |
Germany | Frankfurt | Hamann Andrea |
Germany | Wickert Anika | |
Germany | Wilhelmshaven | Salmon Pablo |
Germany | Gießen | Silaidos Carmina |
Germany | Mainz | Methner Axel |
Germany | Düsseldorf | Goy C |
Germany | Duesseldorf | Roden Michael |
Germany | Doermann Niklas | |
Germany | Frankfurt am Main | Viel Christian |
Germany | Munich | Zischka Hans |
Germany | Mainz | Maull Felicia |
Germany | Magdeburg | Schoenfeld Peter |
Germany | Marburg | Vogt Sebastian |
Germany | Giessen | Sommer Natascha |
Germany | Cologne | Wiesner Rudolf J |
Greece | Heraklion | Tavernarakis Nektarios |
Greece | 15771 | Andreadou Ioanna |
Greece | Thessaloniki | Lazou Antigone |
Greece | Athens | Trougakos Ioannis P |
Greece | Athens | Gumeni Sentiljana |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Chinopoulos Christos |
Hungary | Szeged | Boros Mihaly |
Hungary | Szeged | Ferdinandy Peter |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Horvath G |
Hungary | Budapest | Komlodi Timea |
India | Lucknow | Gayen Jiaur |
India | Kollam | Suravajhala Prashanth |
India | Haryana | Chakrabarti Sasanka |
India | New Delhi | Thakkar Himani |
India | Hyderabad | Jha Rajan Kumar |
India | New Delhi | Vincent Vinnyfred |
India | Varanasi | Sonkar Vijay K |
Iran | Tehran | Shirazi Reza |
Iran | Tehran | Safaei Zahra |
Ireland | Dublin | O'Gorman Donal |
Ireland | Dublin | Porter Richard K |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Zrifin | Sova Marina |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Beer Sheva | Mishmar Dan |
Italia | Udine | Salvadego Desy |
Italien | Padova PD | Szabo Ildiko |
Italy | St. Lorenzen | Harrison David K |
Italy | Bologna | Gasparre G |
Italy | Udine | Grassi B |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Bari | Attimonelli M |
Italy | Ancona | Battino Maurizio |
Italy | Milan | Clementi Emilio |
Italy | Bologna | Genova Maria Luisa |
Italy | Padova | Viscomi Carlo |
Italy | Campobasso | Pallotta Maria Luigia |
Italy | Rome | Scatena Roberto |
Italy | Catania | Messina Angela |
Italy | Bologna | Paterlini S |
Italy | Bolzano | Zanon Alessandra |
Italy | Milano | Bottoni P |
Italy | Padova | Bernardi Paolo |
Italy | Bologna | Porcelli AM |
Italy | Catania | De Pinto Vito |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Monserrato | Isola Raffaella |
Italy | Catania | Magri Andrea |
Japan | Itabashi-ku Tokyo | Tanaka Masashi |
Japan | Sapporo | Matsumoto J |
Kitzbühel | Innsbruck | Fischer Michael J |
Korea | Seoul | Pak Youngmi Kim |
Korea | Incheon | Kwak Hyo Bum |
Korea (South) | Seoul | Lee Hong Kyu |
Latvia | Riga | Volska Kristine |
Latvia | Riga | Vilks Karlis |
Latvia | Riga | Svalbe Baiba |
Latvia | Riga | Zvejniece Liga |
Latvia | Riga | Stelfa Gundega |
Latvia | Riga | Liepins Edgars |
Latvia | Riga | Inashkina I |
Latvia | Riga | Jansone Baiba |
Latvia | Riga | Dambrova Maija |
Luxembourg | Strassen | Devaux Yvan |
Malaysia | Kuala Lumpur | Hassan Hazirah |
Malta | Msida | Vella Joanna |
Mexico | Tlalpan | Rodriguez-Enriquez Sara |
Mexico | Mexico City | Aparicio Trejo OE |
México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
Netherlands | Maastricht | Grevendonk L |
Netherlands | GA Wageningen | Grefte Sander |
New Zealand | Wellington | Berridge Michael V |
New Zealand | Auckland | Ward Marie Louise |
New Zealand | Auckland | Kaur Sarbjot |
New Zealand | Auckland | Hickey Anthony J |
Norway | Bergen | Tronstad Karl Johan |
Norway | Trondheim | Lerfall Joergen |
Norway | Bergen | Roesland Gro Vatne |
Norway | Bergen | Dyrstad Sissel E |
Norway | Bergen | Hoel Fredrik |
Norway | Trondheim | Grill V |
Norway | Bergen | Berge Rolf K |
Norway | Oslo | Eide L |
Norway | Tromsoe | Boardman Neoma T |
Norway | Bergen | Haavik Jan |
Norway | Trondheim | Smenes Benedikte Therese |
Norway | Trondheim | Rognmo O |
Norway | Bergen | Pettersen Nitschke Ina Katrine |
Norway | Bergen | Blindheim Dan Filip |
Poland | Warsaw | Szewczyk Adam |
Poland | Poznan | Jarmuszkiewicz Wieslawa |
Poland | Gdańsk | Kaczor Jan Jacek |
Poland | Warsaw | Bednarczyk Piotr |
Poland | Krakow | Dembinska-Kiec Aldona |
Poland | Warsaw | Sek Aleksandra |
Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
Poland | Warsaw | Wasilewski M |
Poland | Warsaw | Zablocki Krzysztof |
Poland | Warsaw | Wieckowski Mariusz R |
Poland | Lodz | Watala Cezary |
Poland | Warsaw | Dymkowska Dorota |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warszawa | Jaskiewicz Anna |
Poland | Lodz | Labieniec-Watala Magdalena |
Portugal | Porto | Moreira Bruno P |
Portugal | Coimbra | Santos Diana |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Silva Ana Maria |
Portugal | Cantanhede | Amorim Ricardo |
Portugal | Coimbra | Dias Candida |
Portugal | Porto | Beleza Jorge |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Duarte Filipe Valente |
Portugal | Coimbra | Rolo Anabela Pinto |
Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
Portugal | Coimbra | Oliveira Paulo J |
Portugal | Coimbra | Grilo Luis |
Portugal | Coimbra | Minuzzi Luciele M |
Portugal | Lisboa | Macedo Maria Paula |
Portugal | Aveiro | Ferreira Rita Maria P |
Portugal | Porto | Oliveira Jorge |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Cantanhede | Teodoro Joao Soeiro |
Portugal | Cantanhede | Rodrigues Ana Sofia |
Qatar | Doha | Guarch Meritxell Espino |
Republic of Ghana | Cape Coast | Tei BN |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Busan | Kim Hyoung Kyu |
Republic of Korea | Busan | Han Jin |
Republic of Korea | Daejeon | Park SH |
Republic of Serbia | Belgrade | Lalic Nebojsa M |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Republic of Serbia | Novi Sad | Andric Silvana |
Russia | Yoshkar-Ola | Dubinin M |
Russia | Moscow | Vinogradov Andrey D |
Russia | Moscow | Pavlova Nadia |
Serbia | Belgrade | Savkovic U |
Serbia | Belgrade | Misirkic Marjanovic Maja |
Serbia | Belgrade | Mandic M |
Serbia | Belgrade | Djordjevic M |
Singapore | Singapore | Hausenloy Derek J |
Singapore | Singapore | Singh Brijesh Kumar |
Slovakia | Bratislava 45 | Ferko Miroslav |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Sumbalova Zuzana |
Spain | Barcelona | Bravo-Sagua Roberto |
Spain | Barcelona | Gil J |
Spain | Barcelona | Gama Perez Pau |
Spain | Barcelona | Zorzano Antonio |
Spain | Granada | Quiles JL |
Spain | Madrid | Moran M |
Spain | Barcelona | Garcia-Roves Pablo Miguel |
Spain | A Coruña | Blanco FJ |
Spain | Granada | Hidalgo-Gutierrez A |
Spain | Sevilla | Bustos Matilde |
Spain | Madrid | Hernansanz-Agustin Pablo |
Spain | A Coruña | Mayan MD |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | De la Torre Lara J |
Spain | Salamanca | Almeida Angeles |
Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
Spain | Madrid | Rial Eduardo |
Spain | Madrid | Aragones Lopez J |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Perales Jose Carles |
Spain | Barcelona | Goncalo Teixeira da Silva Rui |
Sweden | Stockholm | Nedergaard J |
Sweden | Stockholm | Shabalina Irina G |
Sweden | Stockholm | Cardinale Daniele A |
Sweden | Gothenburg | Bhuvanachandran NS |
Sweden | Stockholm | Larsen Filip J |
Sweden | Lund | Ehinger Johannes K |
Switzerland | Lausanne | Gebara E |
Switzerland | Bern | Wyss RK |
Switzerland | Lausanne | Lagarrigue Sylviane |
Switzerland | Lausanne | Sandi Carmen |
Switzerland | Basel | Fornaro Mara |
Switzerland | Bern | Nuoffer Jean-Marc |
Switzerland | Lausanne | Place Nicolas |
Switzerland | Lausanne | Donnelly Chris |
Switzerland | Lausanne | Morato Fornaguera L |
Switzerland | Zürich | Zaugg Kathrin |
Switzerland | Lausanne | Burtscher Johannes |
Switzerland | Lausanne | Weger M |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Zurich | Gorr Thomas A |
Switzerland | Lausanne | Amati Francesca |
Switzerland | Lausanne | Zalachoras I |
Switzerland | Lausanne | Canto Alvarez Carles |
Switzerland | Basel | Bouitbir Jamal |
Taiwan | Changhua City | Wei Yau-Huei |
The Netherlands | Groningen | Bakker Barbara M |
The Netherlands | BT Amsterdam | Wuest Rob CI |
The Netherlands | Maastricht | Schrauwen Patrick |
The Netherlands | Maastricht | Nabben Miranda |
The Netherlands | Delft | McMillan Duncan GG |
UK | Canterbury, Kent | Gourlay Campbell W |
UK | London | Lane Nick |
UK | London | Rodriguez Enrique |
UK | London | Bettinazzi Stefano |
UK | Cambridge | Brown Guy C |
USA | Fort Collins | Li Puma Lance C |
USA | Milwaukee | Kandel Sunil Mani |
USA | Wakefield | Varricchio Frederick |
USA | Fort Collins | Chicco Adam J |
USA | New Haven | Cavalcanti-de-Albuquerque Joao Paulo |
USA | Winston-Salem | Tomar Dhanendra |
USA | Denton | Dzialowski Edward M |
USA | Torrance | Rossiter Harry B |
USA | East Lansing | Bazil Jason N |
USA | Orlando | Coen Paul M |
USA | Cleveland | Hoppel Charles L |
USA | Orlando | Goodpaster Bret H |
USA | Orlando | Sparks Lauren M |
USA | Denver | Van Hove Johan |
USA | Rochester | Nair K Sreekumaran |
USA | Chicago | Dayanidhi Sudarshan |
USA | Kansas City | Thyfault John P |
USA | El Paso | Bajpeyi Sudip |
USA | Winston-Salem | Gonzalez-Armenta Jenny L |
USA | Gainesville | Leeuwenburgh Christiaan |
USA | Corvallis | Batterson Philip M |
USA | Aurora | Sparagna Genevieve C |
USA | Fairbanks | Coker Robert H |
USA | Seattle | Marcinek DJ |
USA | Iowa City | Chaurasia Bhagirath |
USA | Fairbanks | O'Brien Kristin |
USA | Philadelphia | Towheed Atif |
USA | Norfolk | Lai Nicola |
USA | San Diego | Patel Hemal H |
USA | Little Rock | Borsheim Elisabet |
USA | Little Rock | MacMillan-Crow Lee Ann |
USA | Baltimore | Gonzalez-Freire Marta |
USA | Orlando | Pino Maria F |
USA | Rochester | Lanza Ian R |
USA | Colorado Springs | Jacobs RA |
USA | Aurora | Moreau Kerrie |
USA | Iowa City | Wagner Brett A |
USA | Kansas City | Swerdlow Russell H |
USA | La Jolla | Schilling Jan M |
USA | Akron | Cohen Bruce H |
USA | Jupiter | Trivigno Catherine |
USA | East Lansing | Zhang Yizhu |
USA | Davis | Hellgren Kim T |
USA | Philadelphia | Ganetzky Rebecca |
USA | Pittsburgh | Jurczak Michael J |
USA | Fayetteville | Iyer Shilpa |
USA | Provo | Hancock Chad R |
USA | Little Rock | Parajuli Nirmala |
USA | Baton Rouge | Irving Brian A |
USA | Los Angeles | Kwast Kurt E |
USA | Ann Arbor | Tyrrell Daniel J |
USA | Corvallis | Newsom Sean A |
USA | Columbus | Sims Carrie A |
USA | Pittsburgh | Prochownik Edward V |
USA | New York | Galkin Alexander |
USA | Fayetteville | Rao RR |
USA | Galveston | Durham William J |
USA | Baton Rouge | Hand Steven C |
USA | Indianapolis | Brozinick Joseph T |
USA | Aurora | Ford Ellen |
USA | Nashville | Wasserman David H |
USA | Omaha | Kim Julian KS |
USA | Milwaukee | Dash Ranjan K |
USA | Philadelphia | Falk Marni J |
USA | Atlanta | Jang Young Charles |
USA | Winston-Salem | Ahn Bumsoo |
USA | Puskarich Michael A | |
USA | Bethesda | Sharma Pushpa |
USA | Cleveland | Tandler Bernard |
USA | St. Louis | Abumrad Nada A |
USA | Portland | Garlid Keith D |
USA | Greenville | Neufer P Darrell |
USA | Birmingham | Moellering Douglas R |
USA | Berkeley | Williams Caroline M |
USA | Louisville | Menze Michael A |
USA | Stillwater | Davis Michael S |
USA | San Diego; La Jolla | Molina Anthony JA |
USA | Stanford | Stary Creed |
USA | Durham | Li Pingan Andy |
USA | Warminster | Orynbayeva Zulfiya |
USA | La Jolla | Valentine Joseph Marco |
USA | Davis | Adams Sean H |
USA | Corvallis | Robinson Matthew M |
USA | St. Louis | Pietka Terri A |
... further results |
WG1 Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new data sets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished (move to WG5)
- Analysis is completed and joint publication submitted (with WG5)
- Publication of paper on concepts and terminology (with WG5)
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available (with WG5)
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published (with WG5)
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
- The MITOEAGLE data management system
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system (with WG5)
- An education programme for the use of MITOEAGLE database and management system (move to WG5).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005