Difference between revisions of "MitoOx2"
From Bioblast
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=MitoOx2 | |abbr=MitoOx2 | ||
|description='''Mitochondrial respiration medium, MitoOx2''', developed for oxygraph incubations of [[mitochondrial preparations]] to measure the H<sub>2</sub>O<sub>2</sub> production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H<sub>2</sub>O<sub>2</sub> present) for Amplex UltraRed(R) than e.g. [[MiR05|MiR05]]. Β | |description='''Mitochondrial respiration medium, MitoOx2''', developed for oxygraph incubations of [[mitochondrial preparations]] to measure the H<sub>2</sub>O<sub>2</sub> production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H<sub>2</sub>O<sub>2</sub> present) for Amplex UltraRed(R) than e.g. [[MiR05|MiR05]]. | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia concepts|type=Chemicals | ||
| | }} | ||
|type=Chemicals | {{MitoPedia methods|type=Chemicals | ||
}} | |||
{{MitoPedia O2k and high-resolution respirometry|type=Chemicals | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
Line 12: | Line 14: | ||
|type=Chemicals | |type=Chemicals | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 15:19, 9 February 2016
Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations to measure the H2O2 production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H2O2 present) for Amplex UltraRed(R) than e.g. MiR05.
Abbreviation: MitoOx2
MitoPedia topics: Media for respirometry
Application in HRR
MitoOx2: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx2 at 30Β°C and 37Β°C = 0.95
Chemicals for MitoOx2
Compound | Final conc. | FW | Company, product code and storage |
---|---|---|---|
KCl | 120 mM | 74.55 | Sigma 31248, store at RT |
HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
KH2PO4 | 10 mM | 136.09 | Sigma P-5655, store at RT |
MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 Β°C |
adjust pH to 7.0 (room temperature) with KOH