Difference between revisions of "MitoOx2"
From Bioblast
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::pH of MitoOx2: 7.0 | ::pH of MitoOx2: 7.0 | ||
{| class="wikitable" | |||
|- | |||
! Compound !! Final conc. !! FW !! Company, product code and storage | |||
|- | |||
| KCl || 120 mM || Example || Example | |||
|- | |||
| HEPES free acid || 20 mM || Example || Example | |||
|- | |||
| KH<sub>2</sub>PO<sub>4</sub> || 10 mM || Example || Example | |||
|- | |||
| MgCl<sub>2</sub>|| 2.86 mM || Example || Example | |||
|- | |||
| EGTA free acid || 380.35 || Example || Example | |||
|- | |||
| BSA || 0.025% || Example || Example | |||
|} | |||
Revision as of 12:32, 23 April 2012
- high-resolution terminology - matching measurements at high-resolution
MitoOx2
Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations which yields a higher optical sensitivity for Amplex red.
Abbreviation: MitoOx2
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
MitoOx2: Mitochondrial Respiration Medium
- Oxygen solubility factor of MitoOX2 at 30°C and 37°C = 0.95
- pH of MitoOx2: 7.0
| Compound | Final conc. | FW | Company, product code and storage |
|---|---|---|---|
| KCl | 120 mM | Example | Example |
| HEPES free acid | 20 mM | Example | Example |
| KH2PO4 | 10 mM | Example | Example |
| MgCl2 | 2.86 mM | Example | Example |
| EGTA free acid | 380.35 | Example | Example |
| BSA | 0.025% | Example | Example |
