MitoOx2: Difference between revisions

Revision as of 12:49, 23 April 2012

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MitoOx2

Description

Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations which yields a higher optical sensitivity for Amplex red.

Abbreviation: MitoOx2

MitoPedia methods: Respirometry

MitoPedia topics: Media for respirometry

Application in HRR

MitoOx2: Mitochondrial Respiration Medium

Oxygen solubility factor of MitoOX2 at 30°C and 37°C = 0.95

pH of MitoOx2: 7.0

Chemicals for MitoOx2

Compound	Final conc.	FW	Company, product code and storage
KCl	120 mM	74.55	Sigma 31248, store at RT
HEPES free acid	20 mM	238.31	Sigma H-7523 store at RT
KH₂PO₄	10 mM	136.09	Sigma P-5655, store at RT
MgCl₂	2.86 mM	203.30	Scharlau MA0036, store at RT
EGTA free acid	380.35	380.35	Sigma E-4378, store at RT
BSA essentially fatty acid free	0.025%	-	Sigma A-6003, store at 2 - 8 °C

@@ Line 30: / Line 30: @@
 | KCl || 120 mM || 74.55 || align="right" |Sigma 31248, store at RT
 |-
-| HEPES free acid || 20 mM || 238.31 || Sigma H-7523 store at RT
+| HEPES free acid || 20 mM || 238.31 || align="right" | Sigma H-7523 store at RT
 |-
-| KH<sub>2</sub>PO<sub>4</sub> || 10 mM || 136.09 || Sigma P-5655, store at RT
+| KH<sub>2</sub>PO<sub>4</sub> || 10 mM || 136.09 || align="right" | Sigma P-5655, store at RT
 |-
-| MgCl<sub>2</sub>|| 2.86 mM || 203.30 || Scharlau MA0036, store at RT
+| MgCl<sub>2</sub>|| 2.86 mM || 203.30 || align="right" | Scharlau MA0036, store at RT
 |-
-| EGTA free acid || 380.35 || 380.35 || Sigma E-4378, store at RT
+| EGTA free acid || 380.35 || 380.35 || align="right" | Sigma E-4378, store at RT
 |-
-| BSA essentially fatty acid free || 0.025% || - || Sigma A-6003, store at 2 - 8 °C
+| BSA essentially fatty acid free || 0.025% || - || align="right" | Sigma A-6003, store at 2 - 8 °C
 |}