MitoOx2: Difference between revisions
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! Compound !! Final conc. !! FW !! Company, product code and storage | ! Compound !! Final conc. !! FW !! Company, product code and storage | ||
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| KCl || 120 mM || 74.55 || Sigma 31248, store at RT | | KCl || 120 mM || 74.55 || |align="right"|Sigma 31248, store at RT | ||
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| HEPES free acid || 20 mM || 238.31 || Sigma H-7523 store at RT | | HEPES free acid || 20 mM || 238.31 || Sigma H-7523 store at RT | ||
Revision as of 12:48, 23 April 2012
- high-resolution terminology - matching measurements at high-resolution
MitoOx2
Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations which yields a higher optical sensitivity for Amplex red.
Abbreviation: MitoOx2
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
MitoOx2: Mitochondrial Respiration Medium
- Oxygen solubility factor of MitoOX2 at 30°C and 37°C = 0.95
- pH of MitoOx2: 7.0
Chemicals for MitoOx2
| Compound | Final conc. | FW | Company, product code and storage |
|---|---|---|---|
| KCl | 120 mM | 74.55 | align="right"|Sigma 31248, store at RT |
| HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
| KH2PO4 | 10 mM | 136.09 | Sigma P-5655, store at RT |
| MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
| EGTA free acid | 380.35 | 380.35 | Sigma E-4378, store at RT |
| BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 °C |
