From Bioblast
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
- high-resolution terminology - matching measurements at high-resolution
MitoOx2
Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations to measure the H2O2 production. MitoOx2 yields a higher optical sensitivity and lower "drift" (oxidation of the fluorophore precurcor without H2O2 present) for Amplex UltraRed(R) than e.g. MiR05.
Abbreviation: MitoOx2
MitoPedia topics: Media for respirometry
Application in HRR
MitoOx2: Mitochondrial Respiration Medium for measuring the H2O2 production
- Oxygen solubility factor of MitoOx2 at 30°C and 37°C = 0.95
Chemicals for MitoOx2
| Compound | Final conc. | M [g·mol-1] | Company, product code and storage |
|---|---|---|---|
| KCl | 120 mM | 74.55 | Sigma 31248, store at RT |
| HEPES free acid | 20 mM | 238.31 | Sigma H-7523 store at RT |
| KH2PO4 | 10 mM | 136.09 | Sigma P-5655, store at RT |
| MgCl2 | 2.86 mM | 203.30 | Scharlau MA0036, store at RT |
| EGTA free acid | 0.2 mM | 380.35 | Sigma E-4378, store at RT |
| BSA essentially fatty acid free | 0.025% | - | Sigma A-6003, store at 2 - 8 °C |
adjust pH to 7.0 (room temperature) with KOH
