O2k and HRR
- OROBOROS Oxygraph-2k - unique for high-resolution respirometry (HRR)
- O2k - tested and trusted
- OROBOROS Oxygraph-2k - unique for high-resolution respirometry (HRR)
The O2k for high-resolution respirometry
The OROBOROS Oxygraph-2k provides a unique high-resolution approach for the monitoring of cellular and mitochondrial respiratory function: high-resolution respirometry (HRR). Considering mitochondria as the powerhouses of the cell, HRR introduced the concept of cell ergometry. Are your cells MitoFit?
HRR is applied world-wide in areas ranging from fundamental bioenergetics to the analysis of mitochondrial and metabolic diseases, advancing the rapidly growing field of preventive mitochondrial medicine. Mitochondrial fitness needs to be monitored in a variety of age-related health risks, including cardiovascular and degenerative diseases, such as type 2 diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer.
HRR by the OROBOROS O2k is based on a radically different systems approach to polarographic measurement of oxygen dynamics. HRR rests on several tightly connected instrumental features and concepts integrated by the DatLab software, providing real-time display of oxygen concentration and respiration (oxygen flux). The mechanics, critical selection of materials, and state-of-the-art electronics provide the basis of signal stability of an optimized polarographic oxygen sensor, in an electronically controlled thermal environment with high temperature stability. Calibrations and instrumental controls follow standard operating procedures, with highly automatic real-time analysis by the DatLab software. Scientific support is complementary to the approach of HRR.
From the O2k to the Power-O2k
Multiple O2k-units are offered as a ‘Power-O2k System’ for HRR combined with high-throughput - at high output.
- More details? Consult Power-O2k
The O2k concept
- » OROBOROS Oxygraph-2k: the modular system for high-resolution respirometry and mitochondrial physiology
Open Access in the spirit of Gentle Science.
OROBOROS O2k-Catalogue - a guided tour to the O2k
- O2k is the acronym for the Oxygraph-2k and O2k-Fluorometer for high-resolution respirometry.
O2k-Manual
- Instructions to application and service of the O2k-Core, TIP2k and O2k-MultiSensor Modules.
O2k-Protocols
- O2k-Protocols are complementary to the O2k-Manual and O2k-Publications. Users are encouraged to get involved, contribute and share their expertise.
O2k-Publications
- »4695 O2k-Publications are part of the category Publications, providing a portal for sharing, disseminating and commenting relevant literature in mitochondrial physiology. Bioblast allows the “evolution” of a publication with space for open discussions and extensions of an otherwise static ‘paper’. This applies specifically to Methods and Materials, Discussion, and References sections, which may be limited by space in original publications.
O2k-Network
- O2k-Network Labs from all over the world join to form the unique OROBOROS O2k-Network for high-resolution respirometry.
O2k-Workshops
- Join us in the fast lane to high-resolution respirometry. While the OROBOROS O2k provides the instrumental basis for HRR, successful operation at high accuracy up to the limit of detection depends on a professional application by the technician, scientist or student. Participation in our hands-on introductory OROBOROS O2k-Workshops is recommended to save time and gain from the experience obtained over many years of application in various settings.
The world as a laboratory
- Science and adventure brings the OROBOROS-Team and the O2k to many and sometimes extreme parts of the world in international collaborations - The world as a laboratory.
Mitochondr Physiol Network
- Mitochondrial Physiology Network - the OROBOROS on-line journal.
