- high-resolution terminology - matching measurements at high-resolution
Oxygen flux
Description
Oxygen flux, JO2, is a specific quantity. Oxygen flux is oxygen flow, IO2 [mol·s-1 per system] (an extensive quantity), divided by system size. Flux may be volume-specific (flow per volume [pmol·s-1·mL-1]), mass-specific (flow per mass [pmol·s-1·mg-1]), or marker-specific (flow per mtEU). Oxygen flux (e.g., per body mass, or per cell mass) is distinguished from oxygen flow (per object, or per cell).
Abbreviation: JO2
Reference: Gnaiger 2019 MitoFit Preprint Arch, Gnaiger 2020 MitoPathways, Gnaiger 1993 Pure Appl Chem, Renner 2003 Biochim Biophys Acta
Which SI units should be used?
- Convert various units of volume-specific or mass-specific oxygen flux, JO2, to SI units [nmol·s-1·L-1] = [pmol·s-1·mL-1]
- 1 µmol O2∙min-1∙L-1 ∙ [1000 nmol∙(1 µmol)-1] ∙ [1 min∙(60 s)-1] = 16.67 nmol O2∙s-1∙L-1
- 1 nmol O2∙min-1∙L-1 ∙ [1000 pmol∙(1 nmol)-1] ∙ [1 min∙(60 s)-1] = 16.67 pmol O2∙s-1∙L-1
- 1 nmol O2∙h-1∙L-1 ∙ [1000 pmol∙(1 nmol)-1] ∙ [1 min∙(60 s)-1] ∙ [1 h∙(60 min)-1] = 0.2778 pmol O2∙s-1∙L-1
- 1 natom O∙s-1∙L-1 ∙ [1 nmol O2∙(2 natm O)-1] = 0.5 nmol O2∙s-1∙L-1
- 1 natom O∙min-1∙L-1 ∙ [1000 pmol O2∙(2 natom O)-1] ∙ [1 min∙(60 s)-1] = 8.33 pmol O2∙s-1∙L-1
- 1 mL O2∙min-1∙kg-1 (at STPD) ∙ [1000 µmol∙(22.392 mL)-1] ∙ [1 min∙(60 s)-1] = 0.744 µmol O2∙s-1∙kg-1
- There is a difference between oxygen flux per volume and oxygen concentration change per time
- The oxygen concentration change per time (= rate of concentration change) is expressed in units [µmol O2∙L-1∙s-1].
- By definition the rate of concentration change is zero in an open system at steady-state, when the concentration in the system does not change at any respiratory flux by the sample enclosed in the open system.
- The oxygen concentration change per time (= rate of concentration change) is expressed in units [µmol O2∙L-1∙s-1].
- Oxygen flux per volume (= volume-specific oxygen flux) is expressed in units [µmol O2∙s-1∙L-1] or [nmol O2∙s-1∙L-1]
- By definition the volume-specific oxygen flux is the advancement of reaction per volume of the reaction chamber.
- Oxygen flux per volume (= volume-specific oxygen flux) is expressed in units [µmol O2∙s-1∙L-1] or [nmol O2∙s-1∙L-1]
- Oxygen flux per mass (= mass-specific oxygen flux) is expressed in units [µmol O2∙s-1∙kg-1] = [pmol O2∙s-1∙mg-1]
- By definition the mass-specific oxygen flux is the advancement of reaction per mass of the sample enclosed in the reaction chamber.
- Oxygen flux per mass (= mass-specific oxygen flux) is expressed in units [µmol O2∙s-1∙kg-1] = [pmol O2∙s-1∙mg-1]
MitoPedia concepts: MiP concept, Ergodynamics
MitoPedia methods:
Respirometry
