Palmitoylcarnitine: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Pal | |abbr=Pal | ||
|description=''' | |description='''Palmitoylcarnitine''' is an ester derivative of [[carnitine]] (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production. | ||
|info=[[Cecatto 2023 MitoFit]] | |||
|type=Substrate ETS | |type=Substrate ETS | ||
|methods_type=Substrate ETS | |||
|topics_type=Substrate ETS | |||
}} | }} | ||
__TOC__ | |||
'''Carnitine palmitoyltransferase I''' (CPT1) also known as '''carnitine acyltransferase I''' or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine. | |||
== Application in [[HRR]] == | |||
{{Chemical_description | |||
|abbr=Pal | |||
|trivial name=Palmitoylcarnitine | |||
|complete name=Palmitoyl-DL-carnitine-HCl | |||
|chem formula=C<sub>23</sub>H<sub>46</sub>NO<sub>4</sub> ยท Cl | |||
|molar mass=436.07 | |||
|vendor=Sigma-Aldrich | |||
|product number=P4509 | |||
|store at=-20 ยฐC | |||
|sensitivity= | |||
|cas=6865-14-1 | |||
|h statements= | |||
|h info= | |||
}}<!--:::'''Pal: Palmitoylcarnitine'''ย (Palmitoyl-DL-carnitine-HCl, C<sub>23</sub>H<sub>45</sub>N0<sub>4</sub>HCl); Sigma P 4509, 100 mg, store at -20 ยฐC; M = 436.1 gยทmol<sup>-1</sup>.--> | |||
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# Weigh 8.72 mg of palmitoylcarnitine. | |||
::::# Dissolve in 2 mL H<sub>2</sub>O. | |||
::::# Divide into 0.2 mL portions. | |||
::::# Store frozen at -20 ยฐC. | |||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 2 ยตL using a 10 ยตL syringe (2 mL O2k-chamber). | |||
::::* Final concentration: 10 ยตM. We suggest testing for the optimum concentration for your sample, as palmitoylcarnitine may inhibit respiration ([[Cecatto 2023 MitoFit]]). | |||
{{MitoPedia concepts}} | |||
{{MitoPedia methods | |||
|type=Substrate ETS | |||
}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | }} | ||
ย | {{Labeling | ||
= | |additional=MitoPedia:FAT4BRAIN | ||
ย | }} | ||
ย | |||
ย | |||
Latest revision as of 12:04, 10 April 2024
Description
Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.
Abbreviation: Pal
Reference: Cecatto 2023 MitoFit
Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.
Application in HRR
- Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl; C23H46NO4 ยท Cl), Sigma-Aldrich: P4509, store at -20 ยฐC, CAS: 6865-14-1, M = 436.07 gยทmol-1
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 8.72 mg of palmitoylcarnitine.
- Dissolve in 2 mL H2O.
- Divide into 0.2 mL portions.
- Store frozen at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 ยตL using a 10 ยตL syringe (2 mL O2k-chamber).
- Final concentration: 10 ยตM. We suggest testing for the optimum concentration for your sample, as palmitoylcarnitine may inhibit respiration (Cecatto 2023 MitoFit).
MitoPedia topics:
Substrate and metabolite
Labels:
MitoPedia:FAT4BRAIN