Permeabilized tissue: Difference between revisions
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== Experimental SOP == | |||
Addition of sample to the respirometer chamber: For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again. |
Revision as of 17:55, 18 March 2019
Description
Permeabilized tissue (pti, see also permeabilized muscle fibres, pfi) are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically (e.g., with saponin), for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Abbreviation: pti
Reference: MiPNet11.05; O2k-Publications: Permeabilized tissue
MitoPedia topics:
Sample preparation
Experimental SOP
Addition of sample to the respirometer chamber: For permeabilized tissue, remove the Stopper, add the permeabilized tissue into the O2k-Chamber, close the chamber again.