Rasmussen 2001 Am J Physiol Endocrinol Metab

Has title::Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280:E301-7.

» [[Has info::PMID: 11158934]]

Was written by::Rasmussen UF, Was written by::Rasmussen HN, Was written by::Krustrup P, Was written by::Quistorff B, Was written by::Saltin B, Was written by::Bangsbo J (Was published in year::2001) Was published in journal::Am J Physiol Endocrinol Metab

Abstract: [[has abstract::The aim of the present study was to examine whether parameters of isolated mitochondria could account for the in vivo maximum oxygen uptake (V_O2max) of human skeletal muscle. V_O2max and work performance of the quadriceps muscle of six volunteers were measured in the knee extensor model (range 10-18 mmol O2 x min^-1 x kg^-1 at work rates of 22-32 W/kg). Mitochondria were isolated from the same muscle at rest. Strong correlations were obtained between V_O2max and a number of mitochondrial parameters (mitochondrial protein, cytochrome aa₃, citrate synthase, and respiratory activities). The activities of citrate synthase, succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated from V_O2max. The respiratory chain activity also appeared sufficient. Fully coupled in vitro respiration, which is limited by the rate of ATP synthesis, could account for, at most, 60% of the V_O2max. This might be due to systematic errors or to loose coupling of the mitochondrial respiration under intense exercise.]]

• O2k-Network Lab: Was published by MiPNetLab::DK Copenhagen Quistorff B

MitoEAGLE V_O2max/BME data base

• Human vastus lateralis
• 18 males
• 24 years
• Habitually physically active, no training for competition
• H = 1.8 m
• M = 75 kg
• BME = 0.10
• BMI = 23.1 kg·m^-2
• V_O2max/BM = 54.2 mL·min^-1·kg^-1
• Isolated mitochondria; 25 °C; GS_P; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 123.4 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• 10.5 µM mt-protein/mg m_w
• See also Rasmussen 2000 Mol Cell Biochem, Rasmussen 2001 Pflugers Arch, Rasmussen 2004 Comp Biochem Physiol

---

References: BME and VO2max

» VO2max

{{#ask: Additional label::BME Additional label::VO2max |?Has title=Reference |format=broadtable |limit=5000 |offset=0 |order=ascending }}

Labels: MiParea: MiP area::Respiration, MiP area::mt-Biogenesis;mt-density, MiP area::Exercise physiology;nutrition;life style 

Organism: Organism::Human  Tissue;cell: tissue and cell::Skeletal muscle  Preparation: Preparation::Isolated mitochondria 

Regulation: Topic::Flux control  Coupling state: Coupling states::LEAK, Coupling states::OXPHOS, Coupling states::ET  Pathway: Pathways::F, Pathways::N, Pathways::S, Pathways::Gp, Pathways::NS 

additional label::MitoEAGLE BME, additional label::BMI, additional label::VO2max, additional label::BME