Difference between revisions of "Saponin"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Sap: Saponin''' (from quillaja bark); Sigma | '''Sap: Saponin''' (from quillaja bark); Sigma S7900, 25 g, store at RT. | ||
'''Comment:''' ''Saponin from Quillaja Bark has the product numbers | '''Comment:''' ''Saponin from Quillaja Bark has the product numbers S7900 and S2149, but S2149 is withdrawn from Sigma''. | ||
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | ||
Latest revision as of 15:42, 4 April 2024
- high-resolution terminology - matching measurements at high-resolution
Saponin
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia topics: Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S7900, 25 g, store at RT.
Comment: Saponin from Quillaja Bark has the product numbers S7900 and S2149, but S2149 is withdrawn from Sigma.
Caution: Prepare fresh everyday!
Preparation of 5 mg/mL stock solution (dissolved in BIOPS):
- 1) Weigh 5 mg of saponin.
- 2) Dissolve in 1 mL BIOPS.
→ Use 50 µg saponin/mL BIOPS: add 20 µL of 5 mg/mL saponin stock solution per 2 mL BIOPS.
