Smolkova 2010 J Bioenerg Biomembr

From Bioblast
Revision as of 13:57, 12 June 2022 by Gnaiger Erich (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision β†’ (diff)
Publications in the MiPMap
SmolkovΓ‘ K, Bellance N, Scandurra F, GΓ©not E, Gnaiger E, PlecitΓ‘-HlavatΓ‘ L, JeΕΎek P, Rossignol R (2010) Mitochondrial bioenergetic adaptations of breast cancer cells to aglycemia and hypoxia.

Β» J Bioenerg Biomembr 42:55-67. PMID: 20084539Β»O2k-brief

Smolkova K, Bellance N, Scandurra FM, Genot E, Gnaiger Erich, Plecita-Hlavata L, Jezek P, Rossignol R (2010) J Bioenerg Biomembr

Abstract: O2k-Publications: Topics

Breast cancer cells can survive and proliferate under harsh conditions of nutrient deprivation, including limited oxygen and glucose availability. We hypothesized that such environments trigger metabolic adaptations of mitochondria, which promote tumor progression. Here, we mimicked aglycemia and hypoxia in vitro and compared the mitochondrial and cellular bioenergetic adaptations of human breast cancer (HTB-126) and non-cancer (HTB-125) cells that originate from breast tissue. Using high-resolution respirometry and western blot analyses, we demonstrated that 4 days of glucose deprivation elevated oxidative phosphorylation five-fold, increased the spread of the mitochondrial network without changing its shape, and decreased the apparent affinity of oxygen in cancer cells (increase in c50), whereas it remained unchanged in control cells. The substrate control ratios also remained constant following adaptation. We also observed the Crabtree effect, specifically in HTB-126 cells. Likewise, sustained hypoxia (1% oxygen during 6 days) improved cell respiration in non-cancer cells grown in glucose or glucose-deprived medium (+ 32% and +38%, respectively). Conversely, under these conditions of limited oxygen or a combination of oxygen and glucose deprivation for 6 days, routine respiration was strongly reduced in cancer cells (-36% in glucose medium, -24% in glucose-deprived medium). The data demonstrate that cancer cells behave differently than normal cells when adapting their bioenergetics to microenvironmental conditions. The differences in hypoxia and aglycemia tolerance between breast cancer cells and non-cancer cells may be important when optimizing strategies for the treatment of breast cancer. β€’ Keywords: Mitochondria, Oxidative phosphorylation, Breast cancer, Tumor bioenergetics, Hypoxia, Respirometry

β€’ O2k-Network Lab: AT Innsbruck Gnaiger E, CZ Prague Jezek P, FR Bordeaux Rossignol R



Β» List of O2k-Publications presented as O2k-brief


Cited by

Labels: MiParea: Respiration, mt-Biogenesis;mt-density  Pathology: Cancer 

Organism: Human  Tissue;cell: Fibroblast  Preparation: Intact cells 

Regulation: Oxygen kinetics  Coupling state: ROUTINE, OXPHOS, ET  Pathway: N, NS  HRR: Oxygraph-2k 


Cookies help us deliver our services. By using our services, you agree to our use of cookies.