Difference between revisions of "Sodium phosphate buffer"
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Revision as of 08:49, 24 January 2016
- high-resolution terminology - matching measurements at high-resolution
Sodium phosphate buffer
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast.
Abbreviation: Na-PB
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
- experimental temperature = 28°C
- oxygen solubility factor of Na-PB = 0.95
Na-PB (50 mM, pH 7.1)
Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, FW = 156.01 ) in 500 ml A. dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00) to 7.1.
