Difference between revisions of "Sodium phosphate buffer"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Na-PB | |abbr=Na-PB | ||
|description= Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast. | |description=Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast. | ||
|type=Chemicals | |type=Chemicals | ||
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{{MitoPedia methods | {{MitoPedia concepts|type=Chemicals | ||
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|type=Chemicals | {{MitoPedia methods|type=Chemicals | ||
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{{MitoPedia O2k and high-resolution respirometry|type=Chemicals | |||
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{{MitoPedia topics | {{MitoPedia topics | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 16:36, 8 February 2016
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast.
Abbreviation: Na-PB
MitoPedia topics: Media for respirometry
Application in HRR
- experimental temperature = 28°C
- oxygen solubility factor of Na-PB = 0.95
Na-PB (50 mM, pH 7.1)
Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, FW = 156.01 ) in 500 ml A. dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00) to 7.1.