Sodium phosphate buffer

high-resolution terminology - matching measurements at high-resolution

Sodium phosphate buffer

Description

Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast

Abbreviation: Na-PB

MitoPedia methods: Respirometry 

MitoPedia topics: Media for respirometry 

Application in HRR

• experimental temperature = 28°C

• Oxygen solubility factor of Na-PB = 0.95

Na-PB (50 mM, pH 7.1)

Dissolve 3.9 g NaH₂PO₄ x 2 H₂O (Sigma 71505, FW = 156.01 ) in 500 ml A. dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00)to 7.1.