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  • |description='''Biopsy preservation solution''', for preservation of tissue samples, preparation o
    334 bytes (36 words) - 19:25, 18 April 2016

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  • ...le of Bengt Saltin - Inge Holm and Rob Boushel.JPG|250 px |left|The Golden Biopsy Needle]] == The Golden Biopsy Needle ==
    2 KB (313 words) - 11:14, 15 February 2017
  • ...ion. We analyzed MRC, CS activity and MHC composition in duplicates (intra-biopsy muscle biopsies (inter-biopsy variation) obtained at different sites of the same muscle. Three
    2 KB (316 words) - 15:48, 5 March 2019
  • |description='''Biopsy preservation solution''', for preservation of tissue samples, preparation o
    334 bytes (36 words) - 19:25, 18 April 2016
  • ...mitochondrial respiratory chain can be diagnosed with blood test and skin biopsy, by combining structural (Blue native page) and functional information, wit ...hods to known methods today for diagnoses of mitochondrial disease (muscle biopsy).
    3 KB (412 words) - 10:48, 24 January 2019
  • '''Preparation of 5 mg/mL stock solution (dissolved in''' [[Biopsy preservation solution|BIOPS]]): → Use 50 µg saponin/mL [[Biopsy preservation solution|BIOPS]]: add 20 µL of 5 mg/mL saponin stock solution
    1 KB (152 words) - 15:42, 4 April 2024
  • ...ickey AJ (2013) Hepatic mitochondrial function analysis using needle liver biopsy samples. PLoS One 8:e79097. ...report a method to assess MF using <3 mg of tissue obtained by a Tru-cut® biopsy needle making it suitable for percutaneous application.
    2 KB (350 words) - 17:22, 9 November 2017
  • ...T, Porter C, Saraf MK, Cesani F, Sidossis LS (2015) A percutaneous needle biopsy technique for sampling the supraclavicular brown adipose tissue depot of hu ...he two minor complications noted, which fully resolved. Thus, the proposed biopsy technique can be considered safe with only minimal risk of adverse events.
    2 KB (258 words) - 22:15, 1 August 2018
  • ...me-point of collection, size of fiber bundles, and time that elapsed after biopsy had minor or no effect.
    3 KB (369 words) - 17:13, 7 March 2020
  • ...etal muscle: Adopting high-resolution respirometry to homogenate of needle biopsy tissue samples. Mitochondrion 21:106-12. ...tion respirometry, Human skeletal muscle, Homogenate, Mitochondria, Needle biopsy
    1 KB (200 words) - 14:37, 26 March 2018
  • ...t of tissue to be used is 20mg and that different technicians handling the biopsy do not affect MRC.
    2 KB (227 words) - 16:12, 2 December 2021
  • ...ly, pyruvate (5 mM)-supported respiration was not different between either biopsy technique ±BLEB, whereas BLEB increased succinate-supported respiration in |keywords=Blebbistatin, Mitochondria, Muscle biopsy, Permeabilized fiber, Respiration
    3 KB (390 words) - 12:13, 4 February 2019
  • ...pirometry. Secondary objectives included the feasibility and safety of the biopsy procedure. ...psies taken in one session. The affected muscle was biopsied followed by a biopsy from the ''m. vastus lateralis'' to be used as a control. Measurements of o
    3 KB (439 words) - 15:50, 7 June 2022
  • ...allows for quantification of mitochondrial function ''ex vivo'' from small biopsy samples. muscle from the same biopsy sample. The purpose of this study is to test the functional capacity of mit
    2 KB (285 words) - 09:40, 8 November 2016
  • Patients and methods: We enrolled 16 patients submitted to muscle biopsy in the process of routine diagnostic investigation: four with mitochondrial ...l-CoA-driven respiration; electron transport chain; frozen skeletal muscle biopsy; high-resolution respirometry; mitochondrial diseases; oxygen consumption r
    3 KB (380 words) - 14:41, 21 September 2021
  • ...ng a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive
    886 bytes (124 words) - 05:54, 19 July 2022
  • ...ined in the fasted state and again, with the addition of an adipose tissue biopsy, two hours post-prandial. The effect of RSV on mitochondrial respiration wa
    2 KB (332 words) - 09:13, 27 January 2020
  • ...to use a semi-automatic tool for preparation of a homogenate of the muscle biopsy. We investigated if the PBI-shredder is useful in preparing a muscle homoge
    2 KB (332 words) - 13:07, 27 December 2021
  • Basal cellular oxygen consumption in human AT biopsy samples from the SCV region, known to be [(18)F]fluorodeoxyglucose positron |keywords=Adipose tissue, Biopsy
    2 KB (366 words) - 16:27, 13 November 2017
  • ...tion which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle homogenization at 8000 rotations per minut ...(ET-pathway), FCCP, High resolution oxymetry, MIR05 buffer, Muscle needle biopsy, Respiratory chain complex, Saponin-permeabilized fibers, Ultradispersion
    2 KB (298 words) - 16:27, 13 November 2017
  • ...in veterans with GWI (VGWI). We recruited 42 participants, half VGWI, with biopsy material successfully secured in 36. Impaired MRCF indexed by complex I and
    2 KB (320 words) - 17:30, 17 July 2023
  • ...sue, are now feasible with very small amounts of sample obtained by needle biopsy due to recent advances in the field of high-resolution respirometry. We ill
    3 KB (368 words) - 09:09, 8 November 2016
  • |title=Hoppel CL (2012) Mitochondrial functional testing – muscle biopsy, isolated mitochondria and others. Mitochondr Physiol Network 17.12. Integrated mitochondrial function testing in biopsy material provides one additional approach to the diagnosis of mitochondrial
    4 KB (545 words) - 09:27, 24 November 2015
  • ...lated with the RTCA score. Both RTCA and ''P''-''L'' control efficiency in biopsy samples taken during cold storage have predictive capacity towards the outc
    3 KB (388 words) - 10:05, 23 February 2024
  • ...termination of the mitochondrial respiratory parameters in small (5-10 mg) biopsy samples from human heart.
    1 KB (174 words) - 11:49, 27 February 2013
  • ...with maximal respiration (''r'' = 0.595; ''P'' = 0.003) measured by muscle biopsy respirometry and maximal ATP production (''r'' = 0.643; ''P'' = 0.004) meas ...elet bioenergetics provides a potential supplement or surrogate for muscle biopsy measurement and may be a valuable tool to study mitochondrial involvement i
    3 KB (423 words) - 12:21, 16 September 2021
  • ...olated skeletal muscle mitochondria and permeabilized fibers from the same biopsy to evaluate OXPHOS capacity. ...m a quantitative and qualitative comparison of those two methods in muscle biopsy samples (''vastus lateralis''). The muscle sample from 40 patients was use
    3 KB (452 words) - 18:44, 10 January 2022
  • ...via at 17 200 feet. Subjects come in groups of two, have an acute exposure biopsy then another one after 16 days. The elapsed time from start of first subjec ...right|From biopsy to high-resoltution respirometry. Hans Dreyer taking the biopsy (forground), Catherine Le and Erich Gnaiger preparing for high-resolution r
    6 KB (939 words) - 16:30, 2 January 2024
  • ...o provide a diagnostic step, before or as an alternative to an open muscle biopsy to examine systemic mitochondrial disorders [1]. ...ation and phosphorylation have been identified and possibly avert a muscle biopsy.
    3 KB (480 words) - 15:32, 23 January 2019
  • ...irometry, but these are limited either by need of a large amount of tissue biopsy (isolated mitochondria) or lengthy, time-consuming process of sample prepar ...ons were assessed immediately and in different time intervals after muscle biopsy to test durability of samples. Lastly, we focused on a range of variability
    6 KB (806 words) - 04:58, 18 March 2019
  • PATIENTS AND METHODS: we enrolled 16 patients submitted to muscle biopsy in the process of routine diagnostic investigation: four with mitochondrial |keywords=frozen skeletal muscle biopsy, acetyl-CoA driven respiration (ACoAR), oxygen consumption rate, high-resol
    4 KB (558 words) - 08:43, 8 January 2023
  • ...res, repeated measurements for fibres obtained as subsamples from the same biopsy or muscle tissue are not designed to study intraindividual variability, but
    2 KB (239 words) - 06:01, 19 July 2022
  • ::::## [[Mitochondrial preparation protocols]] including biopsy sampling, tissue storage, cell preparation protocols, etc.
    2 KB (222 words) - 06:12, 19 July 2022
  • ...rom the mouse, we are currently investigating human heart muscle and liver biopsy, but we do not have any published data.'' - '''[[Klaus S| Susanne Klaus]]''
    2 KB (228 words) - 10:51, 9 July 2018
  • :::: To test the hypotheses described above, a portion of the muscle biopsy obtained from the vastus lateralis muscle will be used for assessment of mi ...crucial part of the training. A longitudinal 1-1.5 mm bundle of the muscle biopsy will be removed and mechanically separated into bundles of 5-10 muscle fibr
    6 KB (854 words) - 11:43, 19 June 2019
  • ...odes of postinfectious lactic acidosis. Diagnostic workup including muscle biopsy and postmortem analysis, oxymetric analysis, spectrophotometric enzyme anal
    2 KB (220 words) - 16:21, 28 November 2022
  • ...omogenization and isolation procedure and that large amounts of the tissue biopsy or cultured cells are required for the mitochondrial isolation.
    2 KB (255 words) - 17:53, 22 November 2017
  • Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols
    2 KB (235 words) - 04:16, 23 November 2021
  • ...zed in the intermembrane space, both in isolated cells and tissue samples (biopsy material). Experiments were performed on isolated rat mitochondria or perme ...xpression of AK2 is higher than AK1 in these malignant cells. The study of biopsy material showed that instead of mitochondrial creatine kinase the AK networ
    4 KB (621 words) - 23:52, 17 March 2019
  • ...us to apply a [[high-resolution respirometry]] (HRR) measurement on muscle biopsy to prove a mitochondrial dysfunction as a cause of the disease. Also this a
    2 KB (290 words) - 22:38, 16 March 2019
  • ::::* Biopsy preservation solution with protection of mitochondrial function.
    2 KB (270 words) - 16:11, 23 January 2019
  • File:Greenland3.jpg|Søren in good spirit during preparation for the arm biopsy. File:Greenland4.jpg|The needle biopsy, of which only 2 to 4 mg are required per run in high-resolution respiromet
    8 KB (1,191 words) - 15:21, 1 December 2019
  • ...-resolution respirometry to acquire the integrated respiratory rate of the biopsy.
    2 KB (291 words) - 16:33, 26 March 2018
  • ...itochondrial disease and avoid issues associated with open skeletal muscle biopsy.
    2 KB (278 words) - 15:50, 4 June 2022
  • ...vestigation of oxidative phosphorylation. As shown by the investigation of biopsy samples of two patients with undefined myopathies these fibers are a suitab
    2 KB (279 words) - 11:02, 27 March 2018
  • |keywords=Biopsy, Brown adipose tissue, Mitochondria, Oroboros, Respiration
    2 KB (292 words) - 11:16, 7 March 2020
  • ...he isolation of mitochondria in bioenergetic studies of cultured cells and biopsy samples of muscle. Depending on cell type, 5 to 50 million cells are requir
    2 KB (250 words) - 04:26, 23 November 2021
  • ...lled SK-N-SH, from which it was subcloned, was isolated from a bone marrow biopsy taken from a four year-old female with neuroblastoma. Other human neuroblas
    2 KB (318 words) - 09:07, 23 May 2018
  • ...decreased to congruent with 6.6. Mitochondria were prepared from a needle biopsy sample taken from m. vastus lateralis immediately after the last exercise b
    2 KB (303 words) - 23:29, 8 December 2019
  • The muscle biopsy was obtained using the Bergstrom technique. Immediately after the muscle wa
    2 KB (329 words) - 15:42, 5 March 2019
  • ...obtained from an organ, cells suspended from a cell culture dish, a tissue biopsy, an organ, an individual organism, or a group of organisms (''e.g.'', a num :::: A blood '''sample''' or a biopsy taken from an individual organism. The '''[[sample size]]''', ''N'', corres
    6 KB (956 words) - 16:48, 21 February 2024
  • ...I(N)) and seven healthy controls. All of them underwent a vastus lateralis biopsy in which muscle structure, in vitro mitochondrial respiratory function, unc
    2 KB (302 words) - 15:45, 6 May 2016
  • ...ctions. High-resolution respirometry was performed on permeabilized muscle biopsy preparations. Using four separate and specific substrate titration protocol
    2 KB (326 words) - 12:05, 28 March 2018
  • ...ub>50mito</sub>) in relation to biological sex in human. A skeletal muscle biopsy was donated by nine active women, and ten men matched for maximal oxygen co
    2 KB (330 words) - 17:07, 7 March 2020
  • ...(HVT; 2/d) and 2 wk of reduced-volume training (RVT; 5 sessions). Resting biopsy samples (vastus lateralis) were obtained at baseline and after each phase.
    2 KB (298 words) - 22:12, 1 June 2022
  • ...ion leads to an exchange of the highly conserved Leu196 to Ileu196. Muscle biopsy showed in single fibers decreased COX activity and lowered binding of COX a
    2 KB (310 words) - 15:55, 24 March 2015
  • ...on rate of saponin-skinned muscle fibers isolated from the skeletal muscle biopsy with the specific inhibitors of mitochondrial oxidative phosphorylation com
    2 KB (327 words) - 17:24, 16 January 2021
  • ...as no evidence of the mutation or respiratory complex I defect in a muscle biopsy from the patient's mother. Transmitochondrial cytoplasmic hybrids (cybrids)
    2 KB (300 words) - 16:58, 9 November 2017
  • ...ripheral blood. This method is less-invasively compared to skeletal muscle biopsy. Patients with nephropathies and in combination with non-communicable disea
    2 KB (312 words) - 12:19, 16 September 2021
  • ...thout obesity, but this was independent of NAFLD severity. In repeat liver biopsy sampling in 21 patients with obesity 12 months after bariatric surgery we f
    2 KB (332 words) - 18:14, 23 November 2022
  • ...T) controls (67±1 yrs).V˙ O2 max was measured on a bicycle ergometer and a biopsy obtained from vastus lateralis muscle was permeabilized and prepared for hi
    2 KB (310 words) - 12:22, 8 November 2016
  • ...rate as flux control ratios (FCR) using the Oroboros Oxygraph-2k analyzer. Biopsy samples were permeabilized with saponin and subjected to a protocol adapted
    2 KB (315 words) - 14:01, 30 May 2018
  • ...NMP (OrganOx Metra) for up to 24 h, of which 47 livers were transplanted. Biopsy samples were collected at the end of SCS, at 1 h, 6 h and end of NMP.
    6 KB (786 words) - 08:40, 28 July 2022
  • ...is will significantly expand the scope for screening and exchange of human biopsy samples between research centers, thus providing a new basis for functional
    3 KB (332 words) - 10:53, 23 January 2019
  • ...equencing. A bioenergetics study in fibroblasts was conducted using a skin biopsy taken from a patient with an Arg468His mutation.
    2 KB (359 words) - 12:37, 16 February 2018
  • ...Therefore measuring mitochondrial function may be clinically useful but, a biopsy of organ/tissue is required to measure mitochondrial function and that is i
    2 KB (353 words) - 12:19, 23 January 2019
  • ...or an elective Caesarean section at term with matching controls. A uterine biopsy and fasting blood samples were taken on the day of delivery.
    2 KB (330 words) - 12:36, 27 June 2019
  • ...rounding block of SNP marker homozygosity identified by microarray. Muscle biopsy showed a paucity of mitochondria with ultrastructural abnormalities, mitoch
    2 KB (329 words) - 16:48, 9 November 2017
  • ...alyses, clinical practice is to perform invasive procedures such as muscle biopsy for biochemical and histochemical analyses. Blood cell respirometry is rapi
    2 KB (326 words) - 23:42, 29 February 2020
  • :::: Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an inv
    3 KB (390 words) - 16:47, 12 January 2018
  • ...equencing. A bioenergetics study in fibroblasts was conducted using a skin biopsy taken from a patient with an Arg468His mutation.
    3 KB (376 words) - 12:37, 16 February 2018
  • ...els or lymphocyte analysis rather than invasive muscle and skin fibroblast biopsy, may help predict disease progression in Leigh syndrome patients.
    3 KB (368 words) - 14:39, 5 July 2023
  • ...on. ''Ex vivo'' muscle mitochondrial content and respiration assessed from biopsy samples demonstrated expected differences in obese individuals with (N=18)
    3 KB (346 words) - 14:56, 2 January 2019
  • ...data were compared with those on sham-operated animals (n = 6 each). Liver biopsy samples were subjected to high-resolution respirometry; whole-blood superox
    3 KB (352 words) - 10:21, 8 November 2016
  • ...ion during both progressive arm-cranking and leg-cycling exercises. Muscle biopsy samples were obtained from musculus deltoideus (m. deltoideus) and m. vastu
    3 KB (387 words) - 16:33, 7 March 2020
  • ...ndrial content were measured by quantitative electron microscopy of muscle biopsy samples.
    3 KB (361 words) - 12:13, 28 March 2018
  • ...me). Blood collection for transaminase dosage was carried out, and hepatic biopsy specimens were taken for mitochondrial respiration and histological evaluat
    3 KB (361 words) - 15:06, 25 February 2019
  • (SI). In this secondary analysis, muscle biopsy specimens
    3 KB (361 words) - 14:05, 14 March 2016
  • ...nt. Lipid metabolism proteins, DGAT1, PLIN5, and PNPLA2 were quantified in biopsy samples by western blot.
    2 KB (330 words) - 23:36, 25 December 2015
  • ...ed by isolating and culturing satellite cells from vastus lateralis muscle biopsy samples from patients with ICU-acquired weakness (n = 6) and age-matched he
    3 KB (383 words) - 08:21, 28 March 2018
  • ...Measurement of skeletal muscle mitochondrial respiration requires invasive biopsy to obtain a muscle sample. Peripheral blood mononuclear cell (PBMC) mitocho
    3 KB (392 words) - 14:37, 5 July 2023
  • ...tary electrically stimulated isometric quadriceps-muscle contractions, and biopsy of vastus lateralis muscle.
    3 KB (368 words) - 13:13, 16 August 2018
  • .... Nineteen young men (mean age: 24 ± 4 years) were recruited, and a muscle biopsy was collected to determine mitochondrial respiration from permeabilized mus
    3 KB (379 words) - 16:34, 27 June 2023
  • ...ngolipid, and diacylglycerol content were measured in ''vastus lateralis'' biopsy specimens. We found that EX provided additional improvements in S<sub>I</su
    3 KB (355 words) - 14:28, 8 December 2019
  • ::''' Amount''' Alternatively, enter the sample amount (e.g. biopsy W<sub>m</sub>) if a known amount of sample is added into the chamber. The c
    3 KB (481 words) - 13:35, 8 May 2024
  • ...vity is the citric acid cycle enzyme aconitase. In this study, we examined biopsy specimens of vastus lateralis and triceps brachii in healthy volunteers, to
    3 KB (366 words) - 16:42, 7 March 2020
  • ...hy (<sup>18</sup>F-FDG PET-CT) imaging, and after euthanasia, fresh tumour biopsy samples were taken for oxygen consumption rate measurements (Oroboros Oxygr
    3 KB (373 words) - 11:47, 4 November 2019
  • ...ery. Before the intervention and 72 h after last training session a muscle biopsy was obtained and mitochondrial respiratory capacity, citrate synthase activ
    3 KB (376 words) - 11:37, 3 March 2020
  • ...n Huh-7.5 cells harboring cell culture-derived infectious HCV and in liver biopsy specimens from patients with chronic hepatitis C. The HCV-related HIF-1α s
    3 KB (370 words) - 14:40, 21 July 2020
  • ...ption and the mitochondrial respiratory parameters were realized in tissue biopsy of the Gastrocnemius muscle and Soleus muscle by the technique the High Res
    3 KB (454 words) - 15:50, 11 April 2019
  • ...tic cardiomyopathy, Permeabilized muscle fiber from human atrial appendage biopsy, Permanent oxidative stresss, ROS production, HNE (hydroxynonenal)- and 3-
    3 KB (416 words) - 16:40, 14 October 2019
  • ...ormed at five time points (8AM, 1PM, 6PM, 11PM, 4AM), followed by a muscle biopsy. Mitochondrial oxidative capacity was measured in permeabilized muscle fibe
    3 KB (371 words) - 22:43, 29 October 2020
  • ...lution respirometry]] in permeabilized vastus lateralis fibers obtained by biopsy. Quadriceps femoris muscle cross-sectional area, volume (determined by magn
    3 KB (404 words) - 13:59, 27 March 2018
  • ...e of 13 patients (average age = 5.8 years). In all of them, an open muscle biopsy was performed for diagnosis of their neuromuscular disorder. Mitochondrial
    3 KB (413 words) - 04:29, 23 November 2021
  • ...heart surgery by standard tissue preparation or ''ex vivo'' endomyocardial biopsy (r = 0.9988, p < 0.0001, n = 8), and the mitochondrial oxidativ
    3 KB (387 words) - 13:21, 7 March 2020
  • ...ected before, immediately after and 3 h post HIIE, with the fourth resting biopsy and blood sample taken after four weeks of supervised HIIT (3 training sess
    3 KB (411 words) - 15:52, 29 January 2018
  • |keywords=Horse, Endurance, Biopsy, Skeletal muscle, Mitochondria
    3 KB (416 words) - 16:29, 13 November 2017
  • ...zed in the intermembrane space, both in isolated cells and tissue samples (biopsy material). Experiments were performed on isolated rat mitochondria or perme
    3 KB (406 words) - 18:42, 16 January 2021
  • ...tified ROIs and lactates were measured using the EDGE analyzer. A surgical biopsy of each intestinal ROI was sent for mitochondrial respiratory rate assessme
    3 KB (371 words) - 20:27, 7 March 2020
  • ...n response to long-term physical activity and chronic heart failure (CHF). Biopsy samples of vastus lateralis muscle were obtained from 18 healthy subjects w
    3 KB (465 words) - 21:36, 9 February 2020
  • ...HIIT; 3 sessions per week for 3 weeks) and another blood sample and muscle biopsy were collected. There was no effect of acute exercise or MitoQ on systemic
    3 KB (432 words) - 14:18, 17 January 2023

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