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|description=[[Image:ISS-Filter and Tubing.JPG|right|180px]]'''ISS-Filter and Tubing''', [[ISS-Integrated Suction System]]. {{MitoPedia O2k and high-resolution respirometry

...rial membrane potential with [[Safranin]] when delivered. The filter set [[Filter Set MgG / CaG]] for [[Magnesium green]]® / [[Calcium green]]® measurement {{MitoPedia O2k and high-resolution respirometry

...th different sensors, including photodiode, [[Filter-Cap]] equipped with [[Filter Set AmR]] for [[Amplex® UltraRed|Amplex UltraRed]] and [[TMRM]] measuremen {{MitoPedia O2k and high-resolution respirometry

...otential with [[Safranin|safranin]] and [[Rhodamine 123|rhodamine 123]]. [[Filter Set MgG / CaG]] for [[Magnesium green]] and [[Calcium green]] measurements {{MitoPedia O2k and high-resolution respirometry

...e filters should be used together with [[Fluorescence-Sensor Green]]. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular |info=[https://www.oroboros.at/index.php/product/filter-set-amr/ '''Product details and purchase information''']

|description=[[Image:TIP2k Filter Papers.JPG|right|180px]]'''TIP2k Filter Papers''' (package of 10) to be used for the stoppers during TIP2k titratio |info=[https://www.oroboros.at/index.php/product/tip2k-filter-papers/ '''Product details and purchase information''']

...ether with [[Fluorescence-Sensor Blue]] or [[Smart Fluo-Sensor Blue]]. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular |info=[https://www.oroboros.at/index.php/product/filter-set-saf/ '''Product details and purchase information''']

...D 525 nm (dominant wavelength), photodiode, [[Filter-Cap]] equipped with [[Filter Set AmR]] for [[Amplex® UltraRed|Amplex UltraRed]] measurements when deliv {{MitoPedia O2k and high-resolution respirometry

...ther with [[Fluorescence-Sensor Blue]] or [[Smart Fluo-Sensor Blue]]. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular |info=[https://www.oroboros.at/index.php/product/filter-set-mgg-cag/ '''Product details and purchase information''']

...nsor Green]] and [[Smart Fluo-Sensor Blue]]) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED t |info= [https://www.oroboros.at/index.php/product/filter-cap/ '''Product details and purchase information''']}}

...ousing, [[ISS-Waste Bottle|2 liter waste bottle]], [[ISS-Filter_and_Tubing|filter and tubing]]; for siphoning off excess medium from the O2k-Stopper and for {{MitoPedia O2k and high-resolution respirometry

...ndent information can be viewed for a single chamber or both chambers. The filter can be selected for viewing minimum information, intermittent by default, o {{MitoPedia O2k and high-resolution respirometry

|mitopedia method=Respirometry {{MitoPedia O2k and high-resolution respirometry

...espirometer| O2k-Respirometer]], adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass cha == Mounting a filter cap ==

...concentration [2,3]). Combining optical measurements with high-resolution respirometry further increased the range of analytical opportunities. In particular, flu The O2k-Fluorescence LED2-Module is a LED and filter based fluorometry add-on module to be used together with the O2k. Optical s

...[O2k-Core]] (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass cha == Mounting a filter cap ==

== Calcium Green in high-resolution respirometry ([[HRR]]) == ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc

:::: In this configuration the O2k can be used for high-resolution respirometry and fluorometry. It is not necessary to dismount the Fluorescence-Control U ...k-Guide: Setup - next step - [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''

Application of [[permeabilized muscle fibers]] and [[high-resolution respirometry]] offer a sensitive diagnostic test of mitochondrial dysfunction in small [ ...rcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers

...umental background oxygen flux is an essential standard in high-resolution respirometry. At the same time, an instrumental background experiment is an ultimate tes ...O₂ background test, taking care of the position of the [[TIP2k Filter Papers]]. Additionally, it is better to use the layout ''02-Calibration Bac

{{MitoPedia O2k and high-resolution respirometry}} ...Fluo-Sensor: [[Fluorescence-Sensor Green]] equipped with [[Filter_Set_AmR|Filter Set AmR]].

== Magnesium Green in high-resolution respirometry ([[HRR]]) == ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc

...on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control f ...nal (O₂ slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O₂ background test?'''<br>

|''' Human [[PBMC]] and [[Platelet|Platelets]] - samples for high-resolution respirometry obtained by minimally invasive procedures.'''<br> ...can you reduce the amount of biological sample needed for high-resolution respirometry? '''<br>

:::: A Savitzky-Golay [[smoothing]] filter is used in DatLab as a basis for calculating the time derivative (Flux / Sl |mitopedia method=Respirometry, Fluorometry

...for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods En :::# Fluo-Sensor: [[Smart Fluo-Sensor Blue]] equipped with [[Filter Set Saf]]

=== Background: High-Resolution Respirometry === ...l of bioenergetic protocols for biomedical applications of high-resolution respirometry. The scientific publications and numerous invited lectures at international

...]''' - ''Unlocking cell metabolism: Exploring the power of high-resolution respirometry with the NextGen-O2k'' ...Consensus Meeting 2024 Rome IT]]''' - ''Quality-controlled high-resolution respirometry: bacterial and clinical applications''

::::» [[MitoPedia: Media for respirometry]] ...rcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers

:::: In high-resolution respirometry [[HRR]] the Amplex® UltraRed method (Mohanty et al 1997) is used with the ::::» [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging filter set of the Smart Fluo-Sensors]

...s with a high duplication score are automatically labelled by a plagiarism filter as an alert to readers and authors alike (Citron, Ginsparg 2015). At the st ...2010 by Oroboros Instruments as a glossary and index for [[high-resolution respirometry]]. Bioblast was presented at the Mitochondrial Physiology Conference 2010 (

...rt-965108/ I attended the international Oroboros course on high-resolution respirometry in Innsbruck, Austria, in which I was exposed to new techniques in conducti ...now accepted by the French polar institute (IPEV) and we plan to install a respirometry system in Antarctica! I already worked on the Oroboros in my previous labor

...radley J, Peberdy MA, Ornato JP, Mangino MJ, Tang W (2021) High-resolution respirometry for evaluation of mitochondrial function on brain and heart homogenates in ...uction using the Amplex UltraRed assay in combination with high-resolution respirometry.'''<br>

...atty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Prep |'''Do I need to change the filter set of the O2k-Fluo Smart-Module?'''<br>

...lavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart mus ...ces were normalized to wet weight of manually isolated fibers blotted on a filter paper to remove excess of fluid[17,18]. A recent study showed a relatively

...can you reduce the amount of biological sample needed for high-resolution respirometry? '''<br> ...on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control f