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::::# [[MitoFit protocols]] ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015)

...are validated. The purpose of the proficiency test is also to help improve performance and to determine if the test methods in use are reliable and reproducible ( ...ototype test scenario and expanding the range of application-specific SUIT protocols in the MitoFit-data base. O2k-Core applications are extended by application

...t|100px|Luiza Cardoso]]Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Instrumental quality control and evaluation of mitochondrial respiration medium in high- ...n analytical procedure. The technical repeatability of subsamples reflects instrumental resolution and experimental precision, but is insufficiently reported in mi

...ils of high-resolution respirometry (HRR). Quality control of instrumental performance will be demonstrated by on-line calibration of the oxygen sensors, evaluati ...topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.

...ellular respiratory physiology, and new developments for high instrumental performance. ::::* Introduction to SUIT protocols for simultaneous measurement of O2 flux and H₂O₂ flux

...strumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity | '''Part 1.1: POS service and O2k instrumental setup'''

...sions and new, user-friendly features on [[Run DL-Protocol/Set O2 limit|DL-protocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, ...ds= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Protocols]]

...itochondria were studied by substrate-uncoupler-inhibitor titration (SUIT) protocols and the correlation between the two chambers was calculated by inverted reg ...with r²=0.99 in platelets; 0.96 and 0.95 in fibroblasts applying two SUIT protocols; and 0.99 in cardiac mitochondria. The general offset from identity was les

...rectory (''e.g.'' C:\DatLab_OLD). The standard '''Instrumental and SUIT DL-Protocols''' package is automatically implemented with the simple DatLab programme in ...b performance it is strongly recommended to operate your DatLab files, DL-Protocols and DatLab templates on the PC‘s local HDD.

...and cellular respiratory physiology; new challenges for high instrumental performance. ''' O2k and TIP2k: Real-time instrumental performance'''

...-Workshop includes a basic introduction to quality control of instrumental performance of the Oroboros O2k with integrated on-line analysis, introducing new featu ...velopments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement

:::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::» [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]

...ed fibers and homogenates, an elevated oxygen regime is applied, requiring instrumental background test in the same range of elevated oxygen. |info=[[MiPNet14.06 Instrumental O2 background]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_RespPhysiol]]

...tal sections. Standardized O2k quality controls eliminate interferences by instrumental artifacts. Long-term stability of the polarographic oxygen sensors ensures ...pared to conventional instruments). Multiple substrate/inhibitor titration protocols extend the information gained on various OXPHOS complexes in a single incub

...|Bioblast pdf]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols contents]] ...on-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per

|title=Gnaiger E (2010) Measuring the upper limit of mitochondrial performance - how and why? ...transfer-pathway (ET-pathway) capacity. Estimates of maximum mitochondrial performance (2) provide essential reference points for analysis of metabolic control me

...studies which involve O₂ kinetics, we (1) critically evaluated instrumental limitations of such measurements, (2) developed a new, up-to-date software ...ted anoxic transitions in each respiratory state in the course of [[SUIT]] protocols.

# Setting up the O2k and instrumental service. # Protocols for high-resolution respirometry: focus on combined measurement of respirat

...espirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]]. == Oxygen calibration in instrumental and experimental DatLab files ==

Different layouts are required depending on which instrumental quality control or measurement with biological sample is performed. Learn m |'''Cell culture medium and dithionite titrations – a bad combination for an instrumental O₂ background test?'''<br>

...''. This MiPNet QA-Workshop includes but is not restricted to instrumental performance, and presentations are invited to cover not only applications of the Orobor ...ilable components of QA''': methodological details, exchange of laboratory protocols with updates.

...k are set up in the general lab: Sensor service, sensor test, calibration, instrumental background. .... The first Sherpa volunteers come into the KTM lab. Another series of O2k performance tests.

...nstruments]] and [http://www.wgt.at WGT-Elektronik]. The O2k provides the instrumental basis for [[High-resolution respirometry] (HRR) and represents world-wide t ...he gold standard in high-resolution respirometry (HRR). The main focus in instrumental development is the combination of the Oroboros O2k with additional electroc

...by using different types of Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocols (SUIT1 and SUIT2) where we evaluated the possible deviations between differ ...unlimited titrations and, therefore, the possibility to apply various SUIT protocols designed specifically for a certain project.

...Such conditions must be carefully evaluated, may require modifications of protocols, and need data analysis beyond reporting changes of pH. ==== Instrumental background oxygen flux ====

[[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]] === A1: Diagnostic SUIT protocols of mitochondrial fitness ===

...ium will collaborate on the implementation of refined and novel diagnostic protocols as a milestone towards an internationally recognized center of excellence, ...nd particularly in Tyrol. Comprehensive mitochondrial and exercise testing protocols will be developed and validated in these studies. Case studies will transla

...blications and numerous invited lectures at international conferences were instrumental in introducing the new method to the scientific community (Appendix I). ...of the O2k-Fluorometer by the two companies, and development of diagnostic protocols for methodological evaluation of the instrument with muscle fibres, blood c

...working with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrume File:SC2016 (55).JPG|Trainer Stefan carefully watching the performance of Pedro and Ignazio.

...ps://www.ncbi.nlm.nih.gov/pubmed/17406588 Frezza ''et al.'' (2007), Nature Protocols] ...L|Leon Gallee]]: "Evaluation of mitochondrial respiration as a function of instrumental chamber volume"

...management network will be a strategic innovation to develop harmonization protocols towards generating a rigorously monitored data repository on mitochondrial ...y is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and r

Prepare your TIP2k for Instrumental O₂ background: <br> ....youtube.com/watch?v=VV9pRcK076k O2k-Videosupport: Working with the TIP2k (instrumental O₂ background)]'''«<br>

Different layouts are required depending on which instrumental quality control or measurement with biological sample is performed. Learn m |'''Cell culture medium and dithionite titrations – a bad combination for an instrumental O₂ background test?'''<br>

|'''Protocols that SUIT your research question'''<br> Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupl