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O2k-Barometric Pressure Transducer + ('''O2k-Barometric Pressure Transducer''', … '''O2k-Barometric Pressure Transducer''', A/D converter and digital output to DatLab for continuous recording of [[barometric pressure]] [kPa or mmHg], integrated into the air calibration of the POS ([[MiPNet06.03 POS-calibration-SOP]]). Integral component of the [[O2k-Main Unit]]. The warranty on the accuracy of the signal obtained from the O2k-Barometric Pressure Transducer expires within three years.ure Transducer expires within three years.)
O2k-Electromagnetic Stirrer Twin-Control + ('''O2k-Electromagnetic Stirrer Twin-Contro … '''O2k-Electromagnetic Stirrer Twin-Control''' for smooth rotation of the [[Stirrer-Bar\white PVDF\15x6 mm|stirrer bars]] in the two [[O2k-chamber]]s; with slow-start function to prevent decoupling of the stirrer bar; regulated stirrer speed in the range of 100 to 800 rpm (decoupling may occur at higher stirrer speeds), independent for the two O2k-Chambers; automatic events sent to DatLab when the stirrer is switched on/off or when the rotation seed is changed by the experimenter. Integral component of the [[O2k-Main Unit]].[[O2k-Main Unit]].)
O2k-Main Power Cable + ('''O2k-Main Power Cable''', for connecting the main unit to the power supply.)
O2k-Peltier Temperature Control + ('''O2k-Peltier Temperature Control''': Bui … '''O2k-Peltier Temperature Control''': Built-in electronic thermostat controlling temperature for two [[O2k-chamber]]s in the range of 4 to 47 °C; ±0.002 °C (at room temperature). Continuous recording of the O2k-Copper Block temperature with DatLab. Temperature change from 20 to 30 °C within 15 min; cooling from 30 to 20 °C within 20 min. Integral component of the [[O2k-Main Unit]]. The electronic temperature control of the O2k replaced the conventional water jacket.2k replaced the conventional water jacket.)
Obesity + ('''Obesity''' is a disease resulting from … '''Obesity''' is a disease resulting from excessive accumulation of body fat. In common obesity (non-syndromic obesity) excessive body fat is due to an obesogenic lifestyle with lack of physical exercise ('couch') and caloric surplus of food consumption ('potato'), causing several comorbidities which are characterized as preventable non-communicable diseases. Persistent [[body fat excess]] associated with deficits of physical activity induces a weight-lifting effect on increasing muscle mass with decreasing mitochondrial capacity. Body fat excess, therefore, correlates with [[body mass excess]] up to a critical stage of obesogenic lifestyle-induced [[sarcopenia]], when loss of muscle mass results in further deterioration of physical performance particularly at older age.cal performance particularly at older age.)
OctGM + ('''OctGM''': [[Octanoylcarnitine]] & [[Glutamate]] & [[Malate]]. '''MitoPathway control state:''' [[FN]] '''SUIT protocols:''' [[SUIT-015]], [[SUIT-016]], [[SUIT-017]])
OctGMS + ('''OctGMS''': [[Octanoylcarnitine]] &[[Glutamate]] & [[Malate]]& [[Succinate]]. '''MitoPathway control state:''' [[FNS]] '''SUIT protocols:''' [[SUIT-016]], [[SUIT-017]])
OctM pathway control state + ('''OctM''': [[Octanoylcarnitine]] … '''OctM''': [[Octanoylcarnitine]] & [[Malate]].'''MitoPathway control state:''' F'''SUIT protocols:''' [[SUIT-002]], [[SUIT-015]], [[SUIT-016]], [[SUIT-017]]Respiratory stimulation of the [[Fatty acid oxidation pathway control state| FAO-pathway]], F, by [[fatty acid]] FA in the presence of [[malate]] M. Malate is a [[NADH Electron transfer-pathway state |type N substrate]] (N), required for the F-pathway. In the presence of [[Malate-anaplerotic pathway control state|anaplerotic pathways]] (''e.g.'', [[Malic enzyme|mitochondrial malic enzyme, mtME]]) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see [[SUIT-002]]). The FA concentration has to be optimized to saturate the [[Fatty acid oxidation pathway control state| FAO-pathway]], without inhibiting or uncoupling respiration. Low concentration of [[malate]], typically 0.1 mM, does not saturate the [[N-pathway]]; but saturates the [[Fatty acid oxidation pathway control state |F-pathway]]. High concentration of [[malate]], typically 2 mM, saturates the [[N-pathway]].[[N-pathway]].)
OctPGM pathway control state + ('''OctPGM''': [[Octanoylcarnitine]] … '''OctPGM''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Glutamate]] & [[Malate]].'''MitoPathway control state:''' [[FN]]'''SUIT protocols:''' [[SUIT-002]]:This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<1 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[malate-anaplerotic pathway control state]], M alone), and pyruvate and glutamate is added to compare FAO as the background state with FN as the reference state.O as the background state with FN as the reference state.)
OctPGMS pathway control state + ('''OctPGMS''': [[Octanoylcarnitine]] … '''OctPGMS''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Glutamate]] & [[Malate]] & [[Succinate]].'''MitoPathway control state:''' [[FNS]]'''SUIT protocol:''' [[SUIT-001]], [[SUIT-002]], [[SUIT-015]]This substrate combination supports convergent electron flow to the [[Q-junction]].[[Q-junction]].)

('''OctPGMSGp''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Glutamate]] &)

OctPGMSGp pathway control state + ('''OctPGMSGp''': [[Octanoylcarnitine]] … '''OctPGMSGp''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Glutamate]] & [[Malate]] & [[Succinate]] & [[Glycerophosphate]].'''MitoPathway control state:''' FNSGp'''SUIT protocol:''' [[SUIT-002]]This substrate combination supports convergent electron flow to the [[Q-junction]].[[Q-junction]].)
OctPM pathway control state + ('''OctPM''': [[Octanoylcarnitine]] … '''OctPM''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Malate]].'''MitoPathway control state:''' [[FN]]'''SUIT protocol:''' [[SUIT-002]], [[SUIT-005]]This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[malate-anaplerotic pathway control state]], M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state. the background state with FN as the reference state.)
OctPMS + ('''OctPMS''': [[Octanoylcarnitine]] & [[Pyruvate]] & [[Malate]] & [[Succinate]]. '''MitoPathway control state:''' [[FNS]] '''SUIT protocol:''' [[SUIT-005]])
Octanoate + ('''Octanoate''' (octanoic acid). C8H16O2 Common name: Caprylic acid.)
Octanoylcarnitine + ('''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]].)
Oligomycin + ('''Oligomycin''' (Omy) is an inhibitor of … '''Oligomycin''' (Omy) is an inhibitor of [[ATP synthase]] by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production). The inhibition of ATP synthesis also inhibits respiration. In OXPHOS analysis, Omy is used to induce a [[LEAK respiration]] state of respiration (abbreviated as ''L''(Omy) to differentiate from ''L''(n), LEAK state in the absence of ADP).L''(n), LEAK state in the absence of ADP).)
Optics + ('''Optics''' are the components that are u … '''Optics''' are the components that are used to relay and focus light through a [[spectrofluorometer]] or [[spectrophotometer]]. These would normally consist of lenses and/or concave mirrors. The number of such components should be kept to a minimum due to the losses of light (5-10%) that occur at each surface. light (5-10%) that occur at each surface.)
Ouabain + ('''Ouabain''' (synonym: G-strophantin octa … '''Ouabain''' (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. Low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.ions of ouabain stimulate the Na-K-ATPase.)
Overfitting + ('''Overfitting''' in statistics is the act … '''Overfitting''' in statistics is the act of mistaking noise for a signal. Overfitting makes a model look ‘’better’’ on paper but perform ‘’worse’’ in the real world. This may make it easier to get the model published in an academic journal or to sell to a client, crowding out more honest models from the marketplace. But if the model is fitting noise, it has the potential to hurt the science (quoted from [[Silver 2012 Penguin Press]]).nguin Press]]).)
Overlay of plots - DatLab + ('''Overlay of plots''' is defined in DatLa … '''Overlay of plots''' is defined in DatLab as selection of graph layouts showing identical plots from the two O2k-chambers in each graph. Overlay of plots is selected in [[Graph layout - DatLab |Graph layout]]. Superimposed traces of flux/flow from chambers A and B are then shown in Graph 1, and of concentration in chambers A and B in Graph 2.There are basically two ways to superimpose traces recorded in different experiments: Export of the graphics via windows metafile or export of the data to e.g. a spreadsheet program.If you export via wmf you also can manipulate the graphics but then usually the lines are broken up in different segments. This can be done in various programs like MS Word, Open Office Draw and even in MSPower Point, though this maybe is the worst program to do this. It is better to manipulate them in a proper program like OO Draw, convert it to an unchangeable picture and then import it to a presentation graphics. Anyway, when you import directly to Power point (or other programs), make sure not to import it as a "picture" but as a metafile. Also in some programs you might afterwards have to "break" it up, or accept a "conversion to a MS Draw object" or other similar linguistic inventions of the software gurus. For this option we suggest to do as much as possible directly in DatLab (setting colors, line widths, ..) using the options in "Plots"/"select plots" and "graph"/"options". The “hardcore“ option is to export the data and import it into e.g. a spreadsheet program (MS Excel , OOCalc). It takes longer to have a simple overlay but gives you far less problems later and its easier to make changes later. To do this you can export your dataset "Export"/"Data to Textfile" and then go from there."Data to Textfile" and then go from there.)