Talk:Azide: Difference between revisions
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::: [[Azide]] has been accessed more than ย | ::: [[Azide]] has been accessed more than ย | ||
::::*ย 5,000 times (2016-05-06) | ::::*ย 5,000 times (2016-05-06) | ||
== Application in [[HRR]] (updated instructions) == | |||
:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 gยทmol<sup>-1</sup>. | |||
:::: 2021-02: The preparation instructions were updated to take the volume of the solute (Gp) into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | |||
::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | |||
::::'''Preparation of 4 M storage and stock solution''' (1 mL, dissolved in H<sub>2</sub>O)ย for '''2-mL O2k-chamber''': | |||
::::# Weigh 260 mg of sodium azide. | |||
::::# Dissolve in 900 ยตL H<sub>2</sub>0. | |||
::::# Divide into 0.5 mL portions. | |||
::::# Store at -20 ยฐC. | |||
:::ยป'''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''2-mL O2k-chamber'''): โฅ50 ยตL using a 100 ยตL Hamilton syringe. | |||
::::* Final concentration: โฅ100 mM. | |||
::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)ย for '''0.5-mL O2k-chamber''': | |||
::::# Take 400 ยตL of the 4 M stock solution. | |||
::::# Add 400 ยตL H<sub>2</sub>O. | |||
:::ยป'''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): โฅ25 ยตL using a 100 ยตL Hamilton syringe. | |||
::::* Final concentration: โฅ100 mM. |
Revision as of 12:46, 15 February 2021
Popular Bioblast page
- Azide has been accessed more than
- 5,000 times (2016-05-06)
- Azide has been accessed more than
Application in HRR (updated instructions)
- Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; M = 65.01 gยทmol-1.
- 2021-02: The preparation instructions were updated to take the volume of the solute (Gp) into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
- Caution: Very toxic!
- Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; M = 65.01 gยทmol-1.
- Preparation of 4 M storage and stock solution (1 mL, dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 260 mg of sodium azide.
- Dissolve in 900 ยตL H20.
- Divide into 0.5 mL portions.
- Store at -20 ยฐC.
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): โฅ50 ยตL using a 100 ยตL Hamilton syringe.
- Final concentration: โฅ100 mM.
- Preparation of 2 M stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 400 ยตL of the 4 M stock solution.
- Add 400 ยตL H2O.
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): โฅ25 ยตL using a 100 ยตL Hamilton syringe.
- Final concentration: โฅ100 mM.